Surface adsorption of cannabinoids in LC–MS/MS applications
Adsorption of cannabinoids to different materials has been documented in various different conditions [1, 2, 3, 4, 5, 6, 7, 8, 9, 10]. It is an aspect that can be easily overlooked during the validation of a cannabinoid analysis procedure. During the validation of such a procedure at the Georgia Bureau of Investigation, it was determined that the final reconstitution step before instrumental analysis by liquid chromatography—tandem mass spectrometry (LC–MS/MS) was sensitive to this effect and needed further examination. A study was conducted to measure the effects of a solution and adsorption to glass test tubes during this final step of the procedure.
Up to now most papers on this topic have focused on the materials used during storage of samples such as plastic and glass containers or materials used in a cannabinoid testing procedure such as pipette tips and glass test tubes [1, 2, 3, 4, 5, 6, 7, 8, 9, 10]. The typical approach seems to be combating surface adsorption by...
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 2.Roth KDW, Siegel NA, Johnson RW Jr, Litauszki L, Salvati L Jr, Harrington CA, Wray LK (1996) Investigation of the effects of solution composition and container material type on the loss of 11-nor ∆ 9-THC-9-carboxylic acid. J Anal Toxicol 20:291–300. https://doi.org/10.1093/jat/20.5.291 CrossRefPubMedGoogle Scholar
- 6.Dextraze P, Griffiths WC, Camara P, Audette L, Rosner M (1989) Comparison of fluorescence polarization immunoassay, enzyme immunoassay, and thin-layer chromatography for urine cannabinoid screening. Effects of analyte adsorption and vigorous mixing of specimen on detectability. Ann Clin Lab Sci 19:133–138 (PMID: 2546480) PubMedGoogle Scholar
- 14.United Chemical Technologies (2009) Delta 9-THC (parent), delta 9-hydroxy THC, carboxy-delta 9-THC in whole blood for GC/MS confirmation using: 200 mg clean screen® extraction column. United Chemicals Technologies, BristolGoogle Scholar