Use of polysaccharide-based chiral columns: enantiomeric separation of seven pairs of abused drugs by high-performance liquid chromatography–mass spectrometry
The International Federation of Horse Racing Authorities has published a list of prohibited substances , which includes corticosteroids [2, 3], anabolic steroids [4, 5, 6, 7], diuretics [8, 9], central nervous system stimulants , β-adrenergic receptor blocking agents , narcotics , antiestrogens, polypeptide hormones , etc. The physiochemical and biochemical properties of racemic mixtures and individual isomers differ significantly, prompting us to study their chiral separation and determination. Chiral substances possess a unique feature: despite sharing identical molecular formulae, atom-to-atom linkages, and bonding distances, they cannot be superimposed . It is obvious that in living systems, specific structure-to-activity relationships are required for effective actions on targets (e.g., receptors, enzymes, transporters, DNA, etc.). The physiochemical and biochemical properties of racemic mixtures can differ significantly from those of the...
The authors are thankful to Dr. Ali Ridha, Director General, (Central Veterinary Research Laboratory) for the valuable suggestions and support. We acknowledge the support and assistance of the Equine Forensic Unit management and staff in this project.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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