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Forensic Toxicology

, Volume 37, Issue 1, pp 231–237 | Cite as

Immunoassay-based detection of fentanyl analogs in forensic toxicology

  • Madison Schackmuth
  • Sarah Kerrigan
Short Communication

Abstract

Purpose

The abuse of fentanyl and its analogs (fentalogs) is of growing concern globally. Forensic toxicology laboratories must detect these emerging drugs in biological evidence, and immunoassay is the most widely used screening technique. In this study, the cross-reactivity of 13 fentalogs were investigated using five commercially available kits.

Methods

Dose–response curves were generated using six N-acyl-substituted fentalogs (4-ANPP, acetylfentanyl, butyrylfentanyl, furanylfentanyl, isobutyrylfentanyl, valerylfentanyl), one desphenethyl fentalog (norfentanyl), two piperidine-modified [(+)-cis-3-methylfentanyl, carfentanil], and four phenethyl and piperidine substituted fentalogs (alfentanil, norcarfentanil, remifentanil, sufentanil). Cross-reactivities were estimated for each assay to determine its overall effectiveness for fentalog screening in toxicological samples.

Results and conclusions

Several commercial assays were able to detect either N-acyl or piperidine-modified fentalogs, but none was capable of detecting both. Although this is an inherent disadvantage of the immunoassay approach, it arises from the diverse structural nature of the fentanyl analogs themselves.

Keywords

Fentanyl Fentalogs ELISA Immunoassay Urine 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Japanese Association of Forensic Toxicology and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Forensic ScienceSam Houston State UniversityHuntsvilleUSA

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