BBB-permeable aporphine-type alkaloids in Nelumbo nucifera flowers with accelerative effects on neurite outgrowth in PC-12 cells
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Blood–brain barrier (BBB)-permeable components in the methanolic extract of Nelumbo nucifera flowers showed accelerative effects on neurite outgrowth in PC-12 cells. Among the constituents isolated from N. nucifera flowers in our previous study, aporphine-type alkaloids, lirinidine, asimilobine, N-methylasimilobine, and pronuciferine, showed accelerative effects. Lirinidine, N-methylasimilobine, and an alkaloid-rich diethyl ether fraction at low concentrations increased the expression of mRNAs coding for TrkA, Vav3, and Rac1. In addition, good permeability of asimilobine and N-methylasimilobine was confirmed using an in vitro BBB model. Asimilobine and N-methylasimilobine are considered to be suitable as seed compounds of drugs for Alzheimer’s disease, because of their activity and BBB permeability.
KeywordsNelumbo nucifera Alkaloid PC-12 Neurite outgrowth BBB
Compliance with ethical standards
Conflict of interest
S. Nakashima and H. Matsuda had received research grants from N.T.H Co., Ltd. All authors have read and approved the final manuscript.
We declare that our research included neither human subjects nor animal experiments.
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