Medicinal uses, thin-layer chromatography and high-performance liquid chromatography profiles of plant species from Abomey-Calavi and Dantokpa Market in the Republic of Benin

  • Godfried Dougnon
  • Michiho ItoEmail author
Natural Resource Letter


This study provides a list of popular medicinal plants found in southern Benin (West Africa) with their mode of use, diseases treated, and thin-layer and high-performance liquid chromatography profiles. The list includes 10 of the most widely used plant species from Dantokpa Market (biggest market located in Cotonou) and Abomey-Calavi in the Republic of Benin. Species were identified by the Laboratory of Botany and Applied Ecology, University of Abomey-Calavi. Voucher specimens were deposited in the herbarium of the Experimental Station for Medicinal Plants, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan, and in the National Herbarium of Benin, University of Abomey-Calavi. The list was as follows—Azadirachta indica (Meliaceae), Caesalpinia bonduc (Caesalpiniaceae), Catharanthus roseus (Apocynaceae), Garcinia kola (Clusiaceae), Khaya senegalensis (Meliaceae), Monodora myristica (Annonaceae), Moringa oleifera (Moringaceae), Talinum fruticosum (Talinaceae), Tridax procumbens (Asteraceae), and Xylopia aethiopica (Annonaceae).


Dantokpa Market Abomey-Calavi Traditional healer Market herbalist TLC HPLC 




Compliance with ethical standrads

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11418_2019_1344_MOESM1_ESM.pdf (5.7 mb)
Supplementary material 1 (PDF 5858 kb)


  1. 1.
    Codjia JTC, Assogbadjo AE, Ekué MRM (2003) Diversity and local valorization of Benin’s food forest plant resources. Cah Agric 12:321–331Google Scholar
  2. 2.
    Koudokpon H, Dougnon TV, Bankolé HS et al (2017) Ethnobotanical survey of plants used in the treatment of infections in southern Benin. Health Sci Dis 18:55–71Google Scholar
  3. 3.
    Dougnon GT (2013) Ethnobotanical and ethnopharmacological studies of plants used in the traditional treatment of high blood pressure in central Benin. Thesis for the Grade of Doctor in Pharmacy, University of Abomey-Calavi, Faculty of Health Sciences.
  4. 4.
    Tongco MDC (2007) Purposive sampling as a tool for informant selection. Ethnobot Res Appl 5:147–158CrossRefGoogle Scholar
  5. 5.
    European Directorate for the Quality of Medicines and Healthcare (2017) European Pharmacopoeia 9.0. Council of Europe, Strasbourg Codex, France ( Accessed 22 Feb 2019
  6. 6.
    de Souza S (1988) Flora of Benin: Names of plants in Benin national languages. National University of Benin, Abomey-CalaviGoogle Scholar
  7. 7.
    Alzohairy MA (2016) Therapeutics role of Azadirachta indica (neem) and their active constituents in diseases prevention and treatment. J Evid Based Complement Alternat Med 2016:1–11. CrossRefGoogle Scholar
  8. 8.
    Vani MM, Rao PSS, Varma GN et al (2016) Identification and chemical characterization of Azadirachta indica leaf extracts through thin layer chromatography. Int J Res Eng Technol 5:117–122Google Scholar
  9. 9.
    Schmelzer GH, Gurib-Fakim A (2008) Plant resources of tropical Africa (PROTA) Volume 11. Medicinal plants 1. PROTA Foundation, WageningenGoogle Scholar
  10. 10.
    Parveen A, Zahra Z, Farooqi MQ et al (2017) Phytochemical screening and content determination of different species of genus Caesalpinia belonging to different origin with antidiabetic activity. Pharmacogn J 9:743–749. CrossRefGoogle Scholar
  11. 11.
    Kabesh K, Senthilkumar P, Ragunathan R, Kumar RR (2015) Phytochemical analysis of Catharanthus roseus plant extract and its antimicrobial activity. Int J Pure Appl Biosci 3:162–172Google Scholar
  12. 12.
    Tikhomiroff C, Jolicoeur M (2002) Screening of Catharanthus roseus secondary metabolites by high-performance liquid chromatography. J Chromatogr 955:87–93. CrossRefGoogle Scholar
  13. 13.
    Seanego CT, Ndip RN (2012) Identification and antibacterial evaluation of bioactive compounds from Garcinia kola (Heckel) seeds. Molecules 17:6569–6584. CrossRefGoogle Scholar
  14. 14.
    Tshibangu PT, Kapepula PM, Kapinga MJK et al (2016) Fingerprinting and validation of a LC-DAD method for the analysis of biflavanones in Garcinia kola-based antimalarial improved traditional medicines. J Pharm Biomed Anal 128:382–390. CrossRefGoogle Scholar
  15. 15.
    Sani AA, Alemika TE, Zakama S et al (2013) Phytochemical screening and thin layer chromatography of the leaves of Khaya senegalensis (dry zone mahogany) Meliaceae. J Pharm Bioresources 9:20–23. Google Scholar
  16. 16.
    Zhang H (2008) Characterization of bioactive phytochemicals from the stem bark of African Mahogany Khaya senegalensis (Meliaceae). Dissertation for the Grade of Doctor of Philosophy in Food Technology, Clemson University, South Carolina, USA ( Accessed 12 Mar 2019
  17. 17.
    Moukette BM, Pieme CA, Njimou JR et al (2015) In vitro antioxidant properties, free radicals scavenging activities of extracts and polyphenol composition of a non-timber forest product used as spice: Monodora myristica. Biol Res 48:1–17. CrossRefGoogle Scholar
  18. 18.
    Bueno PR, Alvarez MR, Cruz RO et al (2016) Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) profiling and phytochemical analysis of Euphorbia hirta, Gliricidia sepium and Moringa oleifera methanol extracts. Der Pharma Chem 8:456–461Google Scholar
  19. 19.
    Leone A, Fiorillo G, Criscuoli F et al (2015) Nutritional characterization and phenolic profiling of Moringa oleifera leaves grown in Chad, Sahrawi refugee camps, and Haiti. Int J Mol Sci 16:18923–18937. CrossRefGoogle Scholar
  20. 20.
    Kiranmayee BR, Sirisha GVD, Rachel VK, Jha A (2016) Structural characterization and evaluation of antioxidant activity of isolated phenolic compounds from Talinum fruticosum leaves. Int J Pharm Sci Rev Res 38:75–82Google Scholar
  21. 21.
    Nisha MH (2010) Phytochemical and biological investigation of Tridax procumbens leaves. Thesis for degree of B. Pham, East West University, Aftabnagar, Dhaka of Bangladesh ( Accessed 12 Mar 2019
  22. 22.
    Ekong DEU, Ogan AU (1968) Chemistry of the constituents of Xylopia aethiopica. The structure of xylopic acid, a new diterpene acid. J Chem Soc 69:311–312. Google Scholar
  23. 23.
    Buhian WPC, Rubio RO, Martin-Puzon JJ (2017) Chromatographic fingerprinting and free-radical scavenging activity of ethanol extracts of Muntingia calabura L. leaves and stems. Asian Pac J Trop Biomed 7:139–143. CrossRefGoogle Scholar
  24. 24.
    Fah L, Klotoé JR, Dougnon V et al (2013) Ethnobotanical study of plants used in the treatment of diabetes in pregnant women in Cotonou and Abomey-Calavi (Benin). J Anim Plant Sci 18:2647–2658Google Scholar
  25. 25.
    Adepoju AO, Ogunkunle ATJ, Femi-Adepoju AG (2014) Antifungal activities of seed oil of neem (Azadirachta indica A. Juss). Glob J Biol Agric Heath Sci 3:106–109Google Scholar
  26. 26.
    Ospina Salazar DI, Hoyos Sánchez RA, Orozco Sánchez F et al (2015) Antifungal activity of neem (Azadirachta indica: Meliaceae) extracts against dermatophytes. Acta Biol Colomb 20:201–207. CrossRefGoogle Scholar
  27. 27.
    Bharat P, Sagar R, Sulav R, Ankit P (2015) Investigations of antioxidant and antibacterial activity of leaf extracts of Azadirachta indica. Afr J Biotechnol 14:3159–3163. CrossRefGoogle Scholar
  28. 28.
    Farahna M, Bedri S, Khalid S et al (2010) Anti-plasmodial effects of Azadirachta indica in experimental cerebral malaria: apoptosis of cerebellar Purkinje cells of mice as a marker. N Am J Med Sci. Google Scholar
  29. 29.
    Noorani AA, Gupta K, Bhadada K, Kale MK (2011) Protective effect of methanolic leaf extract of Caesalpinia bonduc (L.) on gentamicin-induced hepatotoxicity and nephrotoxicity in rats. Iran J Pharm Ther 10:21–25Google Scholar
  30. 30.
    Gupta M, Muzumder U, Kumar R (2004) Antitumor activity and antioxidant status of Caesalpinia bonducella against ehrlich ascites carcinoma in swiss albino mice. J Pharmacol Sci 94:177–184CrossRefGoogle Scholar
  31. 31.
    Kumar RS, Kumar KA, Murthy NV (2010) Hepatoprotective and antioxidant effects of Caesalpinia bonducella on carbon tetrachloride-induced liver injury in rats. Int J Plant Sci 1:062–068Google Scholar
  32. 32.
    Tiong S, Looi C, Hazni H et al (2013) Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules 18:9770–9784. CrossRefGoogle Scholar
  33. 33.
    Nayak BS, Pinto PLM (2006) Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complement Altern Med 6:1–6CrossRefGoogle Scholar
  34. 34.
    Akoachere TK, Ndip RN, Chenwi EB et al (2002) Antibacterial effect of Zingiber officinale and Garcinia kola on respiratory tract pathogens. East Afr Med J 79:588–592CrossRefGoogle Scholar
  35. 35.
    Paul CA, Fabian OC, Okechukwu PCU (2014) Antimicrobial effects of bitter kola (Garcinia kola) nut on Staphylococcus aureus, Escherichia coli and Candida albicans. IOSR J Dent Med Sci 13:29–32. CrossRefGoogle Scholar
  36. 36.
    Zhang H, Wang X, Chen F et al (2007) Anticancer activity of limonoid from Khaya senegalensis. Phytother Res 21:731–734CrossRefGoogle Scholar
  37. 37.
    Tatsadjieu LN, Essia-Ngang JJ, Ngassoum MB, Etoa FX (2003) Antibacterial and antifungal activity of Xylopia aethiopica, Monodora myristica, Zanthoxylum xanthoxyloı̈des and Zanthoxylum leprieurii from Cameroon. Fitoterapia 74:469–472. CrossRefGoogle Scholar
  38. 38.
    Gupta R, Mathur M, Bajaj VK et al (2012) Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes. J Diabetes 4:164–171CrossRefGoogle Scholar
  39. 39.
    Elgamily H, Moussa A, Elboraey A et al (2016) Microbiological assessment of Moringa oleifera extracts and its incorporation in novel dental remedies against some oral pathogens. Open Access Maced J Med Sci 4:585–590CrossRefGoogle Scholar
  40. 40.
    Toppo R, Roy BK, Gora RH et al (2015) Hepatoprotective activity of Moringa oleifera against cadmium toxicity in rats. Vet World 8:537–540CrossRefGoogle Scholar
  41. 41.
    Nikkon F, Saud ZA, Rahman MH, Haque ME (2003) In vitro antimicrobial activity of the compound isolated from chloroform extract of Moringa oleifera Lam. Pak J Biol Sci 6:1888–1890CrossRefGoogle Scholar
  42. 42.
    Liao DY, Chai YC, Wang SH et al (2015) Antioxidant activities and contents of flavonoids and phenolic acids of Talinum triangulare extracts and their immunomodulatory effects. J Food Drug Anal 23:294–302. CrossRefGoogle Scholar
  43. 43.
    Olorunnisola OS, Adetutu A, Afolayan AJ, Owoade AO (2016) Effect of methanolic leaf extract of Talinum triangulare (Jacq). Willd. on biochemical parameters in diet induced dyslipidemia Wistar rats. Pharmacogn Mag 12:333–339. Google Scholar
  44. 44.
    Babu Ravindra (2012) Hypoglycemic activity of methanolic extract of Talinum triangulare leaves in normal and streptozotocin induced diabetic rats. J App Pharm Sci 2:197–201. Google Scholar
  45. 45.
    Ravikumar V, Kanchi SS, Devaki T (2005) Effect of Tridax procumbens on liver antioxidant defense system during lipopolysaccharide-induced hepatitis in D-galactosamine sensitised rats. Mol Cell Biochem 269:131–136CrossRefGoogle Scholar
  46. 46.
    Ravikumar V, Shivashangari KS, Devaki T (2005) Hepatoprotective activity of Tridax procumbens against D-galactosamine/lipopolysaccharide-induced hepatitis in rats. J Ethnopharmacol 101:55–60CrossRefGoogle Scholar
  47. 47.
    Asekun OT, Adeniyi BA (2004) Antimicrobial and cytotoxic activities of the fruit essential oil of Xylopia aethiopica from Nigeria. Fitoterapia 75:368–370. CrossRefGoogle Scholar

Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  1. 1.Department of Pharmacognosy, Graduate School of Pharmaceutical SciencesKyoto UniversityKyotoJapan

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