Clerodane diterpenoids from Casearia kurzii and their cytotoxic activities

  • Yuan Shuo
  • Chenyue Zhang
  • Xueyuan Yang
  • Feng Liu
  • Qi Zhang
  • Annan Li
  • Jun Ma
  • Dongho Lee
  • Yasushi Ohizumi
  • Yuanqiang GuoEmail author


A search for bioactive natural products as anticancer lead compounds resulted in the isolation of one previously undescribed and three known clerodane diterpenoids (14) from Casearia kurzii. The structures of these compounds were established by analysis of their NMR, MS, and electronic circular dichroism data. The cytotoxic activities of four compounds against three human cancer cell lines were evaluated. Compound 2 was found to be the most active with an IC50 value of 4.1 μM against HeLa cells, and was selected to investigate the possible cytotoxic mechanism.

Graphic abstract


Casearia kurzii Clerodane diterpenoids Cytotoxic activities Apoptosis Cell cycle 



This work was supported by the National University Student Innovation Program of Nankai University, the National Natural Science Foundation of China (No. U1703107), the Natural Science Foundation of Tianjin, China (No. 16JCYBJC27700), and Hundred Young Academic Leaders Program of Nankai University.

Compliance with ethical standards

Conflict of interest

The authors have declared no competing interests.

Supplementary material

11418_2019_1324_MOESM1_ESM.doc (231 kb)
Appendix A: Supplementary data. 1D and 2D NMR spectra of 1 are available as supplementary data. Supplementary data related to this article can be found online. 1 (DOC 231 kb)


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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  • Yuan Shuo
    • 1
  • Chenyue Zhang
    • 1
  • Xueyuan Yang
    • 1
  • Feng Liu
    • 1
  • Qi Zhang
    • 1
  • Annan Li
    • 1
  • Jun Ma
    • 1
  • Dongho Lee
    • 2
  • Yasushi Ohizumi
    • 3
  • Yuanqiang Guo
    • 1
    Email author
  1. 1.State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Molecular Drug Research, College of PharmacyNankai UniversityTianjinPeople’s Republic of China
  2. 2.Department of Biosystems and Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  3. 3.Kansei Fukushi Research InstituteTohoku Fukushi UniversitySendaiJapan

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