Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test
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Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.
KeywordsIsomiroestrol Pueraria candollei Immunochromatographic strip test White Kwao Krua Standardization
This work was supported by The Thailand Research Fund (IRN61W0005) and Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand. The authors thank Dr. Orawan Monthakantirat, Faculty of Pharmaceutical Sciences, Khon Kaen University for providing the authentic standard of isomiroestrol.
- 4.Anusarnsoondhorn S (1931) The remedy pamphlet of Kwao Krua tuber of Luang Anusornsuntarakromkarnpiset. Upatipongsa Press, Chiang MaiGoogle Scholar
- 9.Lee JH, Kim JY, Cho SH, Jeong JH, Cho S, Park HJ, Baek SY (2017) Determination of miroestrol and isomiroestrol from Pueraria mirifica (White Kwao Krua) in dietary supplements by LC-MS-MS and LC-Q-Orbitrap/MS. J Chromatgr Sci 55(3):214–221Google Scholar
- 11.Kitisripanya T, Krittanai S, Udomsin O, Jutathis K, Komaikul J, Juengwatanatrakul T, Sakamoto S, Tanaka H, Morimoto S, Putalun W (2017) Development of an Enzyme-linked immunosorbent assay for determination of miroestrol using an anti-miroestrol monoclonal antibody. Planta Med 83(10):855–861CrossRefGoogle Scholar
- 13.Kitisripanya T, Inyai C, Komaikul J, Krittanai S, Juengwatanatrakul T, Sakamoto S, Tanaka H, Morimoto S, Putalun W (2017) A lateral flow colloidal gold-based immunoassay for rapid detection of miroestrol in samples of White Kwao Krua, a phytoestrogen-rich plant. J Nat Med 71(4):659–664CrossRefGoogle Scholar