Development of a simple and rapid method for the detection of isomiroestrol in Pueraria candollei by an immunochromatographic strip test

  • Jiranan Chaingam
  • Tharita Kitisripanya
  • Supaluk Krittanai
  • Seiichi Sakamoto
  • Hiroyuki Tanaka
  • Waraporn PutalunEmail author
Original Paper


Pueraria candollei (P. candollei) is a traditional Thai herb widely used for estrogen replacement therapy because it contains many unique chromenes that possess potent estrogenic activity, one of which is known as isomiroestrol. Since isomiroestrol is a promising compound that is solely present in P. candollei, it can be used as an identifying marker for standardization of P. candollei. Here, we developed a lateral-flow immunochromatographic strip (ICS) test using a colloidal gold nanoparticle-conjugated anti-isomiroestrol monoclonal antibody (12C1-mAb) for the detection of isomiroestrol in plant samples and products of P. candollei. The advantages of the developed ICS over an enzyme-linked immunosorbent assay are its simplicity and rapidity, as the ICS test can be completed 15 min after dipping the strip into the analyte solution. The detectable concentration of isomiroestrol was 7.0 µg/mL. Considering the demand for the standardization of P. candollei due to concerns regarding its quality, our ICS test using isomiroestrol as an identifying marker would be effective and useful to assess the presence of isomiroestrol.


Isomiroestrol Pueraria candollei Immunochromatographic strip test White Kwao Krua Standardization 



This work was supported by The Thailand Research Fund (IRN61W0005) and Faculty of Pharmaceutical Sciences, Khon Kaen University, Thailand. The authors thank Dr. Orawan Monthakantirat, Faculty of Pharmaceutical Sciences, Khon Kaen University for providing the authentic standard of isomiroestrol.


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Copyright information

© The Japanese Society of Pharmacognosy 2019

Authors and Affiliations

  1. 1.Faculty of Pharmaceutical SciencesKhon Kaen UniversityKhon KaenThailand
  2. 2.Research Group for Pharmaceutical Activities of Natural Products Using Pharmaceutical Biotechnology (PANPB)National Research University-Khon Kaen UniversityKhon KaenThailand
  3. 3.Department of Pharmacognosy, Faculty of PharmacyMahidol UniversityBangkokThailand
  4. 4.Graduate School of Pharmaceutical SciencesKyushu UniversityFukuokaJapan

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