Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent
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Oxidative stress due to the overproduction of reactive oxygen species plays an important role in the pathogenesis of various diseases. In the present study, we comprehensively evaluated the antioxidant activities of 147 oral formulations of Japanese traditional herbal medicines (Kampo medicines), representing the entire panel of oral Kampo medicines listed in the Japanese National Health Insurance Drug List, using in vitro radical scavenging assays, including the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay, the superoxide anion scavenging activity assay, and the oxygen radical absorption capacity assay. Three of the formulations tested, namely, Tsudosan, Daisaikoto, and Masiningan, showed the most potent in vitro antioxidant activities and were selected for further investigation of their intracellular and in vivo antioxidant effects. The results of the 2′,7′-dichlorodihydrofluorescin diacetate assay demonstrated that all three Kampo medicines significantly inhibited hydrogen peroxide-induced oxidative stress in human hepatocellular liver carcinoma HepG2 cells. In addition, Tsudosan significantly increased the serum biological antioxidant potential values when orally administrated to mice, indicating that it also had in vivo antioxidant activity. The potent antioxidant activity of Tsudosan may be one of the mechanisms closely correlated to its clinical usage against blood stasis.
KeywordsKampo Antioxidant Daisaikoto Tsudosan Masiningan
This investigation was supported by JSPS KAKENHI 15K08003, and the Joint Research grants of Toho University Faculty of Pharmaceutical Sciences.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- 10.Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R (2003) Assays for hydrophilic and lipophilic antioxidant capacity [oxygen radical absorbance capacity (ORACFL)] of plasma and other biological and food samples. J Agric Food Chem 51(11):3273–3279CrossRefGoogle Scholar
- 18.Zhong XF, Huang GD, Luo T, Deng ZY, Hu JN (2012) Protective effect of rhein against oxidative stress-related endothelial cell injury. Mol Med Rep 5(5):1261–1266Google Scholar
- 21.Iizuka A, Iijima OT, Yoshie F, Makino B, Amagaya S, Komatsu Y, Kondo K, Matsumoto A, Itakura H (1998) Inhibitory effects of Dai-saiko-to (Da-Chai-Hu-Tang) on the progression of atherosclerotic lesions in Kurosawa and Kusanagi-hypercholesterolemic rabbits. J Ethnopharmacol 63(3):209–218CrossRefGoogle Scholar
- 24.Brkljača Bottegaro N, Gotić J, Šuran J, Brozić D, Klobučar K, Bojanić K, Vrbanac Z (2018) Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Vet Res 14(1):216. https://doi.org/10.1186/s12917-018-1540-y CrossRefGoogle Scholar
- 25.Ishikawa S, Kubo T, Sunagawa M, Tawaratsumita Y, Sato T, Ishino S, Hisamitsu T (2011) Influence of Chinese herbal medicine on reactive oxygen and blood fluidity in rats. J Jpn Soc Orient Med 62(3):337–346Google Scholar