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Journal of Natural Medicines

, Volume 72, Issue 3, pp 779–783 | Cite as

Cladosporamide A, a new protein tyrosine phosphatase 1B inhibitor, produced by an Indonesian marine sponge-derived Cladosporium sp.

  • Henki Rotinsulu
  • Hiroyuki YamazakiEmail author
  • Shino Sugai
  • Natsuki Iwakura
  • Defny S. Wewengkang
  • Deiske A. Sumilat
  • Michio Namikoshi
Note

Abstract

Cladosporamide A (1), a new protein tyrosine phosphatase (PTP) 1B inhibitor, was isolated together with a known prenylated flavanone derivative (2) from the culture broth of an Indonesian marine sponge-derived Cladosporium sp. TPU1507 by solvent extraction, ODS column chromatography, and preparative HPLC (ODS). The structure of 1 was elucidated based on 1D and 2D NMR data. Compound 1 modestly inhibited PTP1B and T-cell PTP (TCPTP) activities with IC50 values of 48 and 54 μM, respectively. The inhibitory activity of 2 against PTP1B (IC50 = 11 μM) was approximately 2-fold stronger than that against TCPTP (IC50 = 27 μM).

Keywords

Cladosporamide A Marine sponge-derived fungus Cladosporium sp. Protein tyrosine phosphatase 1B Inhibitor 

Notes

Acknowledgements

This work was supported in part by Grants-in-Aid for Scientific Research (16K21310) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan to H. Y., the Takeda Science Foundation to H. Y., the Kanae Foundation for the Promotion of Medical Science to H. Y., and a Grant for Basic Science Research Projects from the Sumitomo Foundation to H. Y. We express our thanks to Mr. T. Matsuki and S. Sato of Tohoku Medical and Pharmaceutical University for measuring mass spectra and to Mr. T. Miura, Y. Bunya, and T. Togashi of Tohoku Medical and Pharmaceutical University for their technical assistance.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Henki Rotinsulu
    • 1
    • 2
  • Hiroyuki Yamazaki
    • 1
    Email author
  • Shino Sugai
    • 1
  • Natsuki Iwakura
    • 1
  • Defny S. Wewengkang
    • 1
    • 2
  • Deiske A. Sumilat
    • 1
    • 3
  • Michio Namikoshi
    • 1
  1. 1.Faculty of Pharmaceutical SciencesTohoku Medical and Pharmaceutical UniversitySendaiJapan
  2. 2.Faculty of Mathematic and Natural SciencesSam Ratulangi UniversityManadoIndonesia
  3. 3.Faculty of Fisheries and Marine ScienceSam Ratulangi UniversityManadoIndonesia

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