Cladosporamide A, a new protein tyrosine phosphatase 1B inhibitor, produced by an Indonesian marine sponge-derived Cladosporium sp.
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Cladosporamide A (1), a new protein tyrosine phosphatase (PTP) 1B inhibitor, was isolated together with a known prenylated flavanone derivative (2) from the culture broth of an Indonesian marine sponge-derived Cladosporium sp. TPU1507 by solvent extraction, ODS column chromatography, and preparative HPLC (ODS). The structure of 1 was elucidated based on 1D and 2D NMR data. Compound 1 modestly inhibited PTP1B and T-cell PTP (TCPTP) activities with IC50 values of 48 and 54 μM, respectively. The inhibitory activity of 2 against PTP1B (IC50 = 11 μM) was approximately 2-fold stronger than that against TCPTP (IC50 = 27 μM).
KeywordsCladosporamide A Marine sponge-derived fungus Cladosporium sp. Protein tyrosine phosphatase 1B Inhibitor
This work was supported in part by Grants-in-Aid for Scientific Research (16K21310) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan to H. Y., the Takeda Science Foundation to H. Y., the Kanae Foundation for the Promotion of Medical Science to H. Y., and a Grant for Basic Science Research Projects from the Sumitomo Foundation to H. Y. We express our thanks to Mr. T. Matsuki and S. Sato of Tohoku Medical and Pharmaceutical University for measuring mass spectra and to Mr. T. Miura, Y. Bunya, and T. Togashi of Tohoku Medical and Pharmaceutical University for their technical assistance.
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