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Journal of Natural Medicines

, Volume 72, Issue 2, pp 563–569 | Cite as

Methylquercetins stimulate melanin biosynthesis in a three-dimensional skin model

  • Kosei YamauchiEmail author
  • Tohru Mitsunaga
Note

Abstract

In a previous study, we found that both synthetic 3-O-methylquercetin (3MQ) and 3,4′,7-O-trimethylquercetin (34′7TMQ) increased extracellular melanin content. 34′7TMQ increased the activity of melanogenic enzymes by stimulating the p38 pathway and the expression of microphthalmia-associated transcription factor (MITF). In contrast, 3MQ increased the activity of melanogenic enzymes without the involvement of MITF, which suggests that 3MQ inhibits the degradation of melanogenic enzymes. In the present study, we investigated the effects of 3MQ and 34′7TMQ on melanogenesis in normal human melanocytes and using a commercial three-dimensional (3D) skin model system. Both 3MQ and 34′7TMQ elongated the dendrites of normal human melanocytes from a Caucasian donor, but did not stimulate melanogenesis in the melanocytes. In the 3D skin model, which included melanocytes from an Asian donor, 3MQ and 34′7TMQ increased and elongated the melanocytes and showed a tendency to stimulate melanogenesis. These results suggest that 3MQ and 34′7TMQ could be put to practical use in skin care products and agents aimed at preventing hair graying.

Keywords

B16 melanoma cells Three-dimensional skin model Normal human melanocyte Methylquercetin 

Notes

Acknowledgements

This work was supported by a Research Fellowship from the Japan Society for the Promotion of Science (JSPS).

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Applied Biological SciencesGifu UniversityGifuJapan

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