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Melanogenesis inhibitory activity of components from Salam leaf (Syzygium polyanthum) extract

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Abstract

In order to identify a novel whitening agent, the methanol extract of S. polyanthum leaf was focused on by the screening test using nine Indonesian medicinal plants for the inhibition of melanogenesis and tyrosinase activity in B16 melanoma cells. Three novel compounds [(1) 1-(2,3,5-trihydroxy-4-methylphenyl)hexane-1-one, (2) 1-(2,3,5-trihydroxy methylphenyl)octane-1-one, and (3) (4E)-1-(2,3,5-trihydroxy-4-methylphenyl)decan-1-one and one known compound [(4) 1-(2,3,5-trihydroxy-4-methylphenyl)decan-1-one were isolated from the methanol extract. Our study demonstrated that S. polyanthum leaf methanol extract at 25–200 μg/mL decreased extracellular melanin formation ca. 20–80%, with high cell viability. Compounds 1–4 were found to be active in melanogenesis and tyrosinase inhibition. Compound 3 was the most active against tyrosinase activity (83.98 μM), particularly when l-tyrosine was the substrate. Compounds 1–4 significantly diminished extracellular melanin formation in B16 melanoma cells (> 80%), with high cell viability. Thus, our study suggested that compounds 1–4 isolated from the methanol extract of S. polyanthum leaf play important roles in decreasing extracellular melanogenesis and inhibiting tyrosinase.

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Authors and Affiliations

  1. The United Graduate School of Agriculture Science, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Andriyana Setyawati, Kae Hirabayashi, Kosei Yamauchi, Hiroyuki Hattori & Tohru Mitsunaga

  2. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tropical Biopharmaca Research Center, Bogor Agriculture University, Bogor, 16128, Indonesia

    Irmanida Batubara & Rudi Heryanto

  3. R&D Biological Science Research, KAO Corporation, Tochigi, 321-3497, Japan

    Hiroshi Hashimoto & Mitsuyuki Hotta

Authors
  1. Andriyana Setyawati
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  2. Kae Hirabayashi
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  3. Kosei Yamauchi
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  4. Hiroyuki Hattori
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  5. Tohru Mitsunaga
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  6. Irmanida Batubara
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  7. Rudi Heryanto
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  8. Hiroshi Hashimoto
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  9. Mitsuyuki Hotta
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Correspondence to Tohru Mitsunaga.

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Setyawati, A., Hirabayashi, K., Yamauchi, K. et al. Melanogenesis inhibitory activity of components from Salam leaf (Syzygium polyanthum) extract. J Nat Med 72, 474–480 (2018). https://doi.org/10.1007/s11418-018-1171-4

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  • DOI: https://doi.org/10.1007/s11418-018-1171-4

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