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Journal of Natural Medicines

, Volume 72, Issue 2, pp 409–423 | Cite as

Inner morphological and chemical differentiation of Boehmeria species

  • Kazi-Marjahan Akter
  • Hye-Jin Kim
  • Atif Ali Khan Khalil
  • Woo Sung Park
  • Mi Kyeong Lee
  • Jong Hee Park
  • Mi-Jeong AhnEmail author
Original Paper

Abstract

The present study was designed to establish quality control parameters for pharmacognostic evaluation and differentiation of eight locally derived Boehmeria species, B. gracilis, B. nivea, B. pannosa, B. platanifolia, B. quelpaertensis, B. spicata, B. splitgerbera, B. tricuspis, and two varieties named B. japonica var. longispica, B. nivea var. concolor, which have been utilized as the folk medicine, ‘Mo-Si-Pool’ in Korea. Although the outer morphological study of these species had been reported, there is no pharmacognostical description yet. Therefore, inner morphological evaluation on leaf midrib, petiole and stem of eight Boehmeria species and two varieties was accomplished along with preliminary phytochemical analysis by HPLC–DAD profiling. The microscopic data showed discriminative inner morphological characteristics such as collenchyma cell layer, thickness of cortex and xylem, frequency of druse and hairs, and number of vascular bundles. The HPLC profiles exhibited more than four characteristic peaks. The molecular ions of the four peaks (1–4) were tentatively identified by ESI–MS, and their structures were identified by NMR spectroscopy to be the flavonoids, rutin (1), isoquercetin (2) and kaempferol-3-O-rutinoside (3), and a phenanthroquinolizidine alkaloid, (−)-cryptopleurine (4). While compounds 1 and 2 were detected in all samples, compound 4 was determined only in B. japonica var. longispica, B. pannosa and B. quelpaertensis and B. splitgerbera. These findings provide the initial scientific criteria for proper identification and establishment of standards for use of Boehmeria species in traditional medicine.

Keywords

Boehmeria species Inner morphological characteristics HPLC profiles Alkaloid 

Notes

Acknowledgements

We would like to thank Prof. Mario G. Ferruzzi at Plants for Human Health Institute, North Carolina State University, NC, USA for editing this manuscript and giving precious advice, and Dr. Jin Hee Park at Nakdonggang National Institute of Biological Resources for collecting Boehmeria species in Ulleung Island, Gyeongbuk Province, Korea. The study was funded by the National Research Foundation of Korea, the Ministry of Science, ICT & Future Planning (NRF-2014R1A1A305070 and 2017R1A2B4008859) and the Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea (SSAC, Grant# no. PJ01106402).

Supplementary material

11418_2017_1164_MOESM1_ESM.pptx (802 kb)
Supplementary material 1 (PPTX 801 kb)

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Kazi-Marjahan Akter
    • 1
  • Hye-Jin Kim
    • 1
  • Atif Ali Khan Khalil
    • 1
  • Woo Sung Park
    • 1
  • Mi Kyeong Lee
    • 2
  • Jong Hee Park
    • 3
  • Mi-Jeong Ahn
    • 1
    Email author
  1. 1.College of Pharmacy and Research Institute of Pharmaceutical SciencesGyeongsang National UniversityJinjuKorea
  2. 2.College of PharmacyChungbuk National UniversityCheongjuKorea
  3. 3.College of PharmacyPusan National UniversityBusanKorea

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