Journal of Natural Medicines

, Volume 72, Issue 1, pp 342–346 | Cite as

AU-1 from Agavaceae plants downregulates the expression of glycolytic enzyme phosphoglycerate mutase

  • Tomofumi FujinoEmail author
  • Akihito Yokosuka
  • Harutaka Ichikawa
  • Toshiyuki Oshima
  • Yoshihiro Mimaki
  • Makio Hayakawa


The spirostanol saponin AU-1 from Agavaceae plants stimulates the expression of the glycolytic enzyme phosphoglycerate mutase (PGAM) in ACHN cells. We hypothesized that this may arise from the downregulation of the NAD+-dependent deacetylase SIRT1. In this article, we showed that, unlike in renal adenocarcinoma cells, AU-1 does not affect the expression of SIRT1 in the normal renal cell-derived cell line HK-2. Consistent with the lack of a downregulation of SIRT1, AU-1 did not upregulate, but rather decreased PGAM expression. Moreover, AU-1 inhibited the increase in PGAM levels that results from the knock-down of SIRT1. Our results suggest that AU-1 may prevent carcinogenesis caused by increased cellular PGAM.


Agave utahensis Phosphoglycerate mutase SIRT1 



We thank Ken Ando for their helpful advice and discussions. This work was supported in part by a grant from the Japan Private School Promotion Foundation.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.


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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2017

Authors and Affiliations

  • Tomofumi Fujino
    • 1
    Email author
  • Akihito Yokosuka
    • 2
  • Harutaka Ichikawa
    • 1
  • Toshiyuki Oshima
    • 1
  • Yoshihiro Mimaki
    • 2
  • Makio Hayakawa
    • 1
  1. 1.Department of Hygiene and Health Sciences, School of PharamacyTokyo University of Pharmacy and Life SciencesHachiojiJapan
  2. 2.Department of Medicinal Pharmacognosy, School of PharamacyTokyo University of Pharmacy and Life SciencesHachiojiJapan

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