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Journal of Natural Medicines

, Volume 72, Issue 1, pp 260–266 | Cite as

Potent apoptosis-inducing activity of erypoegin K, an isoflavone isolated from Erythrina poeppigiana, against human leukemia HL-60 cells

  • Kiyomi Hikita
  • Natsuki Hattori
  • Aya Takeda
  • Yuko Yamakage
  • Rina Shibata
  • Saori Yamada
  • Kuniki Kato
  • Tomiyasu Murata
  • Hitoshi Tanaka
  • Norio KanedaEmail author
Original Paper
  • 213 Downloads

Abstract

Erypoegin K is an isoflavone isolated from the stem bark of Erythrina poeppigiana. It contains a furan group at the A-ring of the core isoflavone structure and can inhibit the activity of glyoxalase I, an enzyme that catalyzes the detoxification of methylglyoxal (MG), a by-product of glycolysis. In the present study, we found that erypoegin K has a potent cytotoxic effect on human leukemia HL-60 cells. Its cytotoxic effect was much stronger than that of a known glyoxalase I inhibitor S-p-bromobenzylglutathione cyclopentyl diester. Conversely, erypoegin K demonstrated weak cytotoxicity toward normal human peripheral lymphocytes. The treatment of HL-60 cells with erypoegin K significantly induced caspase-3 activity, whereas the pretreatment of the cells with caspase-3 inhibitor suppressed erypoegin K-induced cell death. Furthermore, nuclear condensation and apoptotic genome DNA fragmentation were observed in erypoegin K-treated HL-60 cells. These results indicated that the observed cell death was mediated by apoptosis. In addition, the toxic compound MG was highly accumulated in the culture medium of erypoegin K-treated HL-60 cells, suggesting that cell apoptosis was triggered by extracellular MG. The present study showed that erypoegin K has a potent apoptosis-inducing effect on cancerous cell lines, such as HL-60.

Keywords

Erythrina poeppigiana Erypoegin K Apoptosis Glyoxalase I Methylglyoxal HL-60 cells 

Notes

Acknowledgements

This work was partly supported by a Grant-in-Aid for Scientific Research (C) (JP16K08311) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  1. 1.Laboratory of Analytical Neurobiology, Faculty of PharmacyMeijo UniversityNagoyaJapan
  2. 2.Laboratory of Natural Product Chemistry, Faculty of PharmacyMeijo UniversityNagoyaJapan

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