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Journal of Natural Medicines

, Volume 72, Issue 1, pp 211–219 | Cite as

A novel mode of stimulating platelet formation activity in megakaryocytes with peanut skin extract

  • Takahiro Sato
  • Masako Akiyama
  • Ken-ichi Nakahama
  • Shujiro SeoEmail author
  • Masamichi Watanabe
  • Jin Tatsuzaki
  • Ikuo Morita
Original Paper

Abstract

We report in this study novel biochemical activities of peanut skin extract (PEXT) on thrombocytopoiesis. Peanut skin, derived from Arachis hypogaea L., is a traditional Chinese medicine that is used to treat chronic hemorrhage. We have shown that oral administration of PEXT increases the peripheral platelet levels in mice. Recently, we reported a liquid culture system that is useful for investigating megakaryocytopoiesis and thrombocytopoiesis from human CD34+ cells. In this liquid culture system, PEXT was shown to enhance the formation of CD41+/DAPI cells (platelets), but had no effect on the formation of CD41+/DAPI+ cells (megakaryocytes) or on the DNA content. Furthermore, PEXT selectively stimulated proplatelet formation from cultured mature megakaryocytes and phorbol 12-myristate 13 acetate (PMA)-induced formation of platelet-like particles from Meg01 cells. Despite having no influence on the formation of megakaryocyte colony forming units (CFUs), PEXT increased the size of megakaryocytes during their development from CD34+ cells. PEXT showed no effect on the GATA-1 and NF-E2 mRNA levels, which are known to play an important role in thrombocytopoiesis and, based on the results of a pMARE-Luc (pGL3-MARE-luciferase) assay, had no influence on NF-E2 activation in Meg01 cells. These results suggest that PEXT accelerates proplatelet formation from megakaryocytes but does not influence the development of hematopoietic stem cells into megakaryocytes.

Keywords

Platelet Megakaryocyte Peanut skin extract 

Notes

Compliance with ethical standards

Ethics approval

All experimental procedures were performed in accordance with guidelines of the Institutional Animal Care and Use Committee, and were approved by the Animal Research Committee, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University (0060309, 070079).

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Copyright information

© The Japanese Society of Pharmacognosy and Springer Japan KK 2017

Authors and Affiliations

  • Takahiro Sato
    • 1
  • Masako Akiyama
    • 1
  • Ken-ichi Nakahama
    • 1
  • Shujiro Seo
    • 2
    Email author
  • Masamichi Watanabe
    • 2
  • Jin Tatsuzaki
    • 2
  • Ikuo Morita
    • 1
  1. 1.Department of Cellular Physiological Chemistry, Graduate SchoolTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Functional and Phytochemical LaboratoryTokiwa Phytochemical Co., Ltd.Sakura-shiJapan

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