An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide
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Wogonin glucuronide (wogonin 7-O-β-d-glucuronide, Wgn) is widely recognized as a constituent of Scutellariae radix, which is used in Kampo medicines. Wgn has been used for both pharmacological (antifebrile uses and in detoxification) and research purposes. A recombinant antigen-binding fragment (rFab) and an antigen-binding fragment from a monoclonal antibody (mFab) against Wgn were constructed and used in an indirect competitive enzyme-linked immunosorbent assay (icELISA) in this study. The rFab and mFab against Wgn showed both activity and recognition against Wgn. The developed icELISA was validated as a quantitative analytical method to detect Wgn by testing both its utility and its reliability using multiple concentrations of Wgn from S. radix. This approach provides a more economic method to analyze and purify Kampo medicines.
KeywordsWogonin glucuronide (Wgn) Recombinant antigen-binding fragment (rFab) Indirect competitive enzyme-linked immunosorbent assay (icELISA) Anti-Wgn monoclonal antibody (MAb 315A) Scutellariae radix Scutellaria baicalensis
This study was supported, in part, by a Grant-in-Aid from KAKENHI, grant number 16K08296, and the research fund of Kyushu University Foundation.
- 11.Chung HJ, Lim SY, Kim IS, Bu YM, Kim HC, Kim DH, Yoo HH (2012) Simultaneous determination of baicalein, baicalin, wogonin, and wogonoside in rat plasma by LC-MS/MS for studying the pharmacokinetics of the standardized extract of Scutellariae Radix. Bull Korean Chem Soc 33:177–182CrossRefGoogle Scholar
- 19.Umetsu M, Tsumoto K, Hara M, Ashish K, Goda S, Adschiri T, Kumagai I (2003) How additives influence the refolding of immunoglobulin-folded proteins in a stepwise dialysis system. Spectroscopic evidence for highly efficient refolding of a single-chain Fv fragment. J Biol Chem 278:8979–8987CrossRefPubMedGoogle Scholar
- 20.Miyaichi Y, Tomimori T (1994) Studies on the constituents of Scutellaria species XVI. On the phenol glycosides of the root of Scutellaria baicalensis Georgi. Nat Med 48:215–218Google Scholar
- 21.Miyaichi Y, Tomimori T (1995) Studies on the constituents of Scutellaria species XVII. Phenol glycosides of the root of Scutellaria baicalensis Georgi (2). Nat Med 49:350–353Google Scholar