The action and mechanism of myrislignan on A549 cells in vitro and in vivo
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Myrislignan is a natural compound with little pharmacological study. In our investigation, we investigated the effect of myrislignan in the induction of apoptosis in A549 cells in vitro and in vivo. Myrislignan inhibited the proliferation of A549 cells in a dose- and time-dependent manner assayed by MTT. In addition, Hoechst flow cytometry showed that myrislignan significantly induced apoptosis and cell cycle arrest in A549 cells. The apoptosis and anti-cell proliferation was mediated by the activation of mitogen-activated protein kinase and the inhibition of epidermal growth factor receptor signal pathway, change of mitochondrial membrane potential, the releasing of c-Myc, the downregulation of the level of the anti-apoptotic protein Bcl-2, and the upregulation of the level of the pro-apoptotic protein Bax. In conclusion, those results reveal a potential mechanism for the anti-cancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a promising compound for the treatment of lung cancer.
KeywordsHuman lung cancer cells Apoptosis Cell cycle arrest Myrislignan EGFR-MAPK
Financial support for this research was provided by State Administration of Traditional Chinese Medicine “Twelfth Five Year Plan” Key Specialty (Chinese Medicine Geriatrics). Technological support for this research was provided by Shanghai Key Laboratory of Clinical Geriatric Medicine.
Compliance with ethical standards
Conflict of interest
No potential conflicts of interest were disclosed.
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