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Role of compost biochar amendment on the (im)mobilization of cadmium and zinc for Chinese cabbage (Brassica rapa L.) from contaminated soil

  • Mukesh Kumar AwasthiEmail author
  • Quan Wang
  • Hongyu Chen
  • Tao Liu
  • Sanjeev Kumar Awasthi
  • Yumin Duan
  • Sunita Varjani
  • Ashok Pandey
  • Zengqiang ZhangEmail author
Technological Innovation for Soil/Sediment Remediation

Abstract

Purpose

This study aims to assess the effect of amendment of an alkaline Zn, Cd-contaminated soil with compost of wheat straw biochar (CB) (4, 8, 12, and 18%) and sludge and different dosages of nitrogen. In addition, this study aimed to substantially mitigate the bioavailability of Zn and Cd for Chinese cabbage (Brassica rapa L.) from smelter-contaminated soils.

Materials and methods

This study was based on Chinese cabbage (B. rapa L.) growth for phytoremediation of soil toxic metals (TMs) (Zn and Cd) in different dosages of CB nitrogen (50, 150, 250, and 300 kg ha−1) and soil (T1) alone as well as different nitrogen dosages (50-T18, 150-T19, 250-T20, and 300-T21 kg ha−1) of chemical fertilizer (CF). The total and bioavailability of Zn and Cd were measured in shoot and root dry weight biomass, and soil in initial and after harvesting of Chinese cabbage. The chlorophyll content, pH, electrical conductivity, cation exchange capacity, and dissolved organic carbon content were measured to understand their role for plant growth and bioavailability of Zn and Cd. The phytoremediation of Zn and Cd was estimated by diethylene triamine pentaacetic acid (DTPA)-extractable method using smelter-contaminated soils amended with four different dosages of biochar.

Results and discussion

The CB application considerably reduced the DTPA-extractable Zn from contaminated soils, while in comparison to the control and CF treatment evidently mobilized the Zn in soil by approximately 4.17%. The maximum solubility of Zn of 23.46 and 26.78% was obtained with the control and CF-applied treatments in soils, respectively. The DTPA-extractable Cd concentration became elevated with increasing CB dosage, and a significantly higher Cd concentration was recorded in T6 compared to the control and chemical fertilizer-applied treatments. In the cases of higher dosages of CB, the Zn translocation in plants from soil was evidently reduced, but a lower CB amendment increased Zn levels by 5.15% in the shoot and 4.78% in the root, respectively. The changes in the soil EC, pH, and CEC by the amendment of CB could be the main reasons for the (im)mobilization of TMs in contaminated soil.

Conclusions

Finally, the results confirmed that the CB amendment, especially with 300 kg ha−1 nitrogen, could reduce maximum bioavailability of Zn and Cd and the ecological risk of contaminated soil with TMs.

Keywords

Alkaline soils Chinese cabbage Compost biochar Immobilization Toxic metals 

Abbreviations

DTPA

Diethylene triamine pentaacetic acid

CB

Composted biochar

WHC

Water holding capacity

TKN

Total Kjeldahl nitrogen

DW

Dry weight

TMs

Toxic metals

CEC

Cation exchange capacity

Cd

Cadmium

Zn

Zinc

EC

Electrical conductivity

Notes

Acknowledgments

The authors are thankful to all laboratory colleagues and research staff members for their constructive advice and help.

Funding information

The authors are grateful for the financial support from Research Fund for International Young Scientists from National Natural Science Foundation of China (Grant No. 31750110469), China and The Introduction of talent research start-up costs (No. Z101021803), College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.

References

  1. Adriano DC, Wenzel WW, Vangronsveld J, Bolan NS (2004) Role of assisted natural remediation in environmental cleanup. Geoderma 122:121–142CrossRefGoogle Scholar
  2. Ahmad M, Lee SS, Yang JE, Ro HM, Lee YH, Ok YS (2012) Effects of soil dilution and amendments (mussel shell, cow bone, and biochar) on Pb availability and phytotoxicity in military shooting range soil. Ecotoxicol Environ Saf 79:225–231CrossRefGoogle Scholar
  3. Ahmad M, Rajapaksha AU, Lim JE, Zhang M, Bolan N, Mohan D, Vithanage M, Lee SS, Ok YS (2014) Biochar as a sorbent for contaminant management in soil and water: a review. Chemosphere 99:19–33CrossRefGoogle Scholar
  4. Ali A, Guo D, Mahar A, Ma F, Li RH, Shen F, Wang P, Zhang Z (2017a) Streptomyces pactum assisted phytoremediation in Zn/Pb smelter contaminated soil of Feng County and its impact on enzymatic activities. Sci Rep 7:46–87CrossRefGoogle Scholar
  5. Ali A, Guo D, Zhang Y, Sun X, Jiang SC, Guo ZY, Huang H, Liang W, Li RH, Zhang Z (2017b) Using bamboo biochar with compost for the stabilization and phytotoxicity reduction of heavy metals in mine-contaminated soils of China. Sci Rep 7:26–90CrossRefGoogle Scholar
  6. ASTM 1125. Standard test methods for electrical conductivity and resistivity of waterGoogle Scholar
  7. Awasthi MK, Wang M, Chen H, Wang Q, Zhao J, Ren X, Li DS, Awasthi SK, Shen F, Li R, Zhang Z (2017a) Heterogeneity of biochar amendment to improve the carbon and nitrogen sequestration through reduce the greenhouse gases emissions during sewage sludge composting. Bioresour Technol 224:428–438CrossRefGoogle Scholar
  8. Awasthi MK, Wang Q, Chen H, Wang M, Ren X, Zhao J, Li J, Guo D, Li DS, Awasthi SK, Sun X, Zhang Z (2017b) Evaluation of biochar amended biosolids co-composting to improve the nutrient transformation and its correlation as a function for the production of nutrient-rich compost. Bioresour Technol 237:156–166CrossRefGoogle Scholar
  9. Beesley L, Dickinson N (2011) Carbon and trace element fluxes in the pore water of an urban soil following greenwaste compost, woody and biochar amendments, inoculated with the earthworm Lumbricus terrestris. Soil Biol Biochem 43(1):188–196Google Scholar
  10. Beesley L, Marmiroli M (2011) The immobilization and retention of soluble arsenic, cadmium and zinc by biochar. Environ Pollut 159:474–480CrossRefGoogle Scholar
  11. Beesley L, Moreno-Jiménez E, Gomez-Eyles JL (2010) Effects of biochar and green waste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil. Environ Pollut 158:2282–2287CrossRefGoogle Scholar
  12. Boisson J, Ruttens A, Mench M, Vangronsveld J (1999) Evaluation of hydroxyapatite as a metal immobilizing soil additive for the remediation of polluted soils. Part 1. Influence of hydroxyapatite on metal exchangeability in soil, plant growth and plant metal accumulation. Environ Pollut 104:225–233CrossRefGoogle Scholar
  13. Brewer CE, Unger R, Schmidt-Rohr K, Brown RC (2011) Criteria to select biochars for field studies based on biochar chemical properties. Bioenergy Res 4:312–323CrossRefGoogle Scholar
  14. Cheng SF, Hseu ZY (2001) In-situ immobilization of cadmium and lead by different amendments in two contaminated soils. Water Air Soil Pollut 140:73–84CrossRefGoogle Scholar
  15. Du LN, Yang YY, Li G, Wang S, Jia XM, Zhao YH (2010) Optimization of heavy metal-containing dye Acid Black 172 decolorization by Pseudomonas sp. DY1 using statistical designs. Int Biodeterior Biodegr 64:566–573Google Scholar
  16. Garau G, Castaldi C, Santona L, Deiana P, Melis P (2007) Influence of red mud, zeolite and lime on heavy metal immobilization, culturable heterotrophic microbial populations and enzyme activities in a contaminated soil. Geoderma 142:47–57CrossRefGoogle Scholar
  17. Guo GL, Zhou QX, Ma LQ (2006) Availability and assessment of fixing additives for the in situ remediation of heavy metal contaminated soils: a review. Environ Monit Assess 116:513–528CrossRefGoogle Scholar
  18. He HD, Tam NFY, Yao AJ, Qiu RL, Li WC, Ye ZH (2017) Growth and Cd uptake by rice (Oryza sativa) in acidic and Cd contaminated paddy soils amended with steel slag. Chemosphere 189:247–254CrossRefGoogle Scholar
  19. ISO 14235 (1998) Soil quality—determination of organic carbon by sulfochromic oxidation. International Organization for Standardization, GenèveGoogle Scholar
  20. ISO 14870 (2001) Soil quality—extraction of trace elements by buffered DTPA solution. International Organization for Standardization, GenèveGoogle Scholar
  21. Janoš P, Vávrová J, Herzogová L, Pilařová V (2010) Effects of inorganic and organic amendments on the mobility (leachability) of heavy metals in contaminated soil: a sequential extraction study. Geoderma 159:335–341CrossRefGoogle Scholar
  22. Karami N, Clemente R, Moreno-Jimenez E, Lepp NW, Beesley L (2011) Efficiency of green waste compost and biochar soil amendments for reducing lead and copper mobility and uptake to ryegrass. J Hazard Mater 191:41–48CrossRefGoogle Scholar
  23. Keller C, Marchetti M, Rossi L, Lugon-Moulin N (2005) Reduction of cadmium availability to tobacco (Nicotiana tabacum) plants using soil amendments in low cadmium-contaminated agricultural soils: a pot experiment. Plant Soil 276:69–84CrossRefGoogle Scholar
  24. Khan S, Waqasa M, Ding F, Shamshad I, Arpd PHH, Li G (2015) The influence of various biochars on the bioaccessibility and bioaccumulation of PAHs and potentially toxic elements to turnips (Brassica rapa L.). J Hazard Mater 300:243–253CrossRefGoogle Scholar
  25. Khan KY, Ali B, Cui XQ, Feng Y, Yang X, Stoffella PJ (2017) Impact of different feedstocks derived biochar amendment with cadmium low uptake affinity cultivar of pak choi (Brassica rapa sb. chinensis L.) on phytoavoidation of Cd to reduce potential dietary toxicity. Ecotoxicol Environ Saf 141:129–138CrossRefGoogle Scholar
  26. Killi D, Kavdir, Y (2013) Effects of olive solid waste and olive solid waste compost application on soil properties and growth of Solanum lycopersicum. Int Biodeterior Biodegr 82:157–165Google Scholar
  27. Kulikowska D, Gusiatin ZM, Bulkowska K, Kierklo K (2015) Humic substances from sewage sludge compost as washing agent effectively remove Cu and Cd from soil. Chemosphere 136:42–49CrossRefGoogle Scholar
  28. Kumar R, Bhatia D, Singh R, Bishnoi NR, Kumar, R, Bhatia D, Singh R, Bishnoi NR (2012) Metal tolerance and sequestration of Ni(II), Zn(II) and Cr(VI) ions from simulated and electroplating wastewater in batch process: kinetics and equilibrium study. Int Biodeterior Biodegr 66:82–90Google Scholar
  29. Kumpiene J, Lagerkvist A, Maurice C (2008) Stabilization of As, Cr, Cu, Pb and Zn in soil using amendments - A review. Waste Manag 28:215–225Google Scholar
  30. Lahori AH, Zhang ZQ, Guo ZY, Mahar A, Li RH, Awasthi MK, Sial TA, Kumbhar F, Wang P, Shen F, Zhao JC, Huang H (2017a) Potential use of lime combined with additives on (im)mobilization and phyto-availability of heavy metals from Pb/Zn smelter contaminated soils. Ecotoxicol Environ Saf 145:313–323CrossRefGoogle Scholar
  31. Lahori AH, Zhang ZQ, Guo ZY, Li RH, Mahar A, Awasthi MK, Wang P, Shen F, Kumbhar F, Sial TA, Zhao JC, Gue D (2017b) Beneficial effects of tobacco biochar combined with mineral additives on (im) mobilization and (bio) availability of Pb, Cd, Cu and Zn from Pb/Zn smelter contaminated soils. Ecotoxicol Environ Saf 145:528–538CrossRefGoogle Scholar
  32. Lahori AH, Gue ZY, Zhang ZQ, Li RH, Mahar A, Awasthi MK, Shen F, Sial TA, Kumbhar F, Wang P, Jiang SC (2017c) Use of biochar as amendment for soil heavy metals immobilization: prospects and challenges. Pedosphere 27:991–1014CrossRefGoogle Scholar
  33. Lee SH, Lee JS, Choi YJ, Kim JG (2009) In situ stabilization of cadmium-, lead-, and zinc-contaminated soil using various amendments. Chemosphere 77:1069–1075CrossRefGoogle Scholar
  34. Lei K, Giubilato E, Critto A, Pan H, Lin C (2016) Contamination and human health risk of lead in soils around lead/zinc smelting areas in China. Environ Sci Pollut Res 23:13128–13136CrossRefGoogle Scholar
  35. Li JF, Li YM, Wu MJ, Zhang ZY, Lü JH (2013) Effectiveness of low temperature biochar in controlling the release and leaching of herbicides in soil. Plant Soil 370(1):333–344Google Scholar
  36. Li Z, Ma Z, van der Kuijp TJ, Yuan Z, Huang L (2014) A review of soil heavy metal pollution from mines in China: pollution and health risk assessment. Sci Total Environ 468:843–853CrossRefGoogle Scholar
  37. Liang B, Lehmann J, Solomon D, Kinyangi J, Grossman J, O'Neill B, Skjemstad JO, Thies J, Luizão FJ, Petersen J (2006) Black carbon increases cation exchange capacity in soils. Soil Sci Soc Am J 70:1719–1730CrossRefGoogle Scholar
  38. Liang XF, Xu YM, Wang L, Sun YB, Lin DS, Sun Y, Qin X, Wan Q (2013) Sorption of Pb2+ on mercapto functionalized sepiolite. Chemosphere 90:548–555CrossRefGoogle Scholar
  39. Lindsay WL, Norvell WA (1978) Development of a DTPA test for zinc, iron, manganese and copper. Soil Sci Soc Am J 42:421–428CrossRefGoogle Scholar
  40. Liu J, Schulz H, Brandl S, Miehtke H, Huwe B, Glaser B (2012) Short-term effect of biochar and compost on soil fertility and water status of a Dystric Cambisol in NE Germany under field conditions. Soil Sci Plant Nutr 175:1–10CrossRefGoogle Scholar
  41. Liu C, Wang H, Tang X, Guan Z, Reid BJ, Rajapaksha AU, Ok YS, Sun H (2016) Biochar increased water holding capacity but accelerated organic carbon leaching from a sloping farmland soil in China. Environ Sci Pollut Res 23:995–1006CrossRefGoogle Scholar
  42. Lombi E, Zhao FJ, Zhang GY, Sun B, Fitz W, Zhang H, McGrath SP (2002) In situ fixation of metals in soils using bauxite residue: chemical assessment. Environ Pollut 118:435–443CrossRefGoogle Scholar
  43. Lu KP, Yang X, Gielen G, Bolan N, Ok YS, Niazi NK, Xu S, Yuan GD, Chen X, Zhang XK, Liu D, Song ZL, Liu XY, Wang HL (2017) Effect of bamboo and rice straw biochars on the mobility and redistribution of heavy metals (Cd, Cu, Pb and Zn) in contaminated soil. J Environ Manag 186:285–292CrossRefGoogle Scholar
  44. Luo X, Liu G, Xia Y, Chen L, Jiang Z, Zheng H, Wang Z (2017) Use of biochar-compost to improve properties and productivity of the degraded coastal soil in the Yellow River Delta, China. J Soils Sediments 17:780–789CrossRefGoogle Scholar
  45. Mahar A, Wang P, Ali A, Guo ZY, Awasthi MK, Lahori AH, Wang Q, Shen F, Li RH, Zhang ZQ (2016) Impact of CaO, fly ash, sulfur and Na2S on the (im)mobilization and phyto-availability of Cd, Cu and Pb in contaminated soil. Ecotoxicol Environ Saf 134:116–123CrossRefGoogle Scholar
  46. Mando OA, Zombré NP (2001) Use of compost to improve soil properties and crop productivity under low input agricultural system in West Africa. Agric Ecosyst Environ 84:259–266CrossRefGoogle Scholar
  47. Mehmood T, Bibi I, Shahid M, Niazi NK, Murtaza B, Wang H, Ok YS, Sarkar B, Javed MT, Murtaza G (2017) Effect of compost addition on arsenic uptake, morphological and physiological attributes of maize plants grown in contrasting soils. J Geochem Explor 178:83–91CrossRefGoogle Scholar
  48. Ming H, Naidu R, Sarkar B, Lamb DT, Liu Y, Megharaj M, Sparks D (2016) Competitive sorption of cadmium and zinc in contrasting soils. Geoderma 268:60–68CrossRefGoogle Scholar
  49. Mohamed I, Zhang GS, Li ZG, Liu Y, Chen F, Dai K (2015) Ecological restoration of an acidic Cd contaminated soil using bamboo biochar application. Ecol Eng 84:67–76CrossRefGoogle Scholar
  50. Nejad DZ, Jung MC (2017) The effects of biochar and inorganic amendments on soil remediation in the presence of hyperaccumulator plant. Int J Energy Environ Eng 8:317–329CrossRefGoogle Scholar
  51. Oustriere N, Marchand L, Rosette G, Friesl-Hanl W, Mench M (2017) Wood-derived-biochar combined with compost or irongrit for in situ stabilization of Cd, Pb, and Zn in a contaminated soil. Environ Sci Pollut Res 24:7468–7481CrossRefGoogle Scholar
  52. Park JH, Choppala GK, Bolan NS, Chung JW, Chuasavathi T (2011) Biochar reduces the bioavailability and phytotoxicity of heavy metals. Plant Soil 348:439–451CrossRefGoogle Scholar
  53. Park JH, Choppala GH, Lee SJ, Bolan N, Chung JW, Edraki M (2013) Comparative sorption of Pb and Cd by biochars and its implication for metal immobilization in soil. Water Air Soil Pollut 224:1711CrossRefGoogle Scholar
  54. Pruvot C, Douay F, Herve F, Waterlot C (2006) Heavy metals in soil, crops and grass as a source of human exposure in the former mining areas. J Soils Sediments 6:215–220CrossRefGoogle Scholar
  55. Schulz H, Glaser B (2012) Effects of biochar compared to organic and inorganic fertilizers on soil quality and plant growth in a greenhouse experiment. J Plant Nutr Soil Sci 175:410–422CrossRefGoogle Scholar
  56. Schulz H, Durst G, Glaser B (2013) Positive effects of composted biochar on plant growth and soil fertility. Agron Sustain Dev 33:817–827CrossRefGoogle Scholar
  57. Seehausen ML, Gale NV, Dranga S, Hudson V, Liu N, Michener J, Thurston E, Williams C, Smith SM, Thomas SC (2017) Is there a positive synergistic effect of biochar and compost soil amendments on plant growth and physiological performance? Agronomy 7.  https://doi.org/10.3390/agronomy7010013
  58. Shen F, Lia RM, Ali A, Mahar A, Guo D, Li RH, Sun X, Awasthi MK, Wang Q, Zhang ZQ (2017) Spatial distribution and risk assessment of heavy metals in soil near a Pb/Zn smelter in Feng County, China. Ecotoxicol Environ Saf 139:254–262CrossRefGoogle Scholar
  59. Sherene T (2009) Effect of dissolved organic carbon (DOC) on heavy metal mobility in soils. Nat Environ Pollut Technol 8:817–821Google Scholar
  60. Sochan A, Bieganowski A, Ryzak M, Dobrowolski R, Bartmiñski P (2012) Comparison of soil texture determined by two dispersion units of Mastersizer 2000. Int Agrophys 26:99–102CrossRefGoogle Scholar
  61. Spokas KA (2010) Review of the stability of biochar in soils: predictability of O: C molar ratios. Carbon Manag 1:289–303CrossRefGoogle Scholar
  62. Sun YB, Li Y, Xu YM, Liang XF, Wang L (2015) In situ stabilization remediation of cadmium (Cd) and lead (Pb) co-contaminated paddy soil using bentonite. Appl Clay Sci 105-106:200–206CrossRefGoogle Scholar
  63. USEPA (1986) Test methods for evaluating solid waste. Laboratory Manual Physical/Chemical Methods, vol. 1A. US Govt. Print. Office, Washington, DC (SW-846)Google Scholar
  64. USEPA Method 9045D. Soil and waste pHGoogle Scholar
  65. USEPA Method 9080. Cation-exchange capacity of soils (ammonium acetate)Google Scholar
  66. Wang BL, Wang CP, Li J, Sun HW, Xu Z (2014) Remediation of alkaline soil with heavy metal contamination using tourmaline as a novel amendment. J Environ Chem Eng 2:1281–1286CrossRefGoogle Scholar
  67. Wang Q, Awasthi MK, Ren X, Zhao J, Li R, Shen F, Zhang Z (2017) Effect of calcium bentonite on Zn and Cu mobility and their accumulation in vegetable growth in soil amended with compost during consecutive planting. Environ Sci Pollut Res 24:15645–15654CrossRefGoogle Scholar
  68. Warnock DD, Lehmann J, Kuyper TW, Rillig MC (2007) Mycorrhizal responses to biochar in soil concepts and mechanisms. Plant Soil 300:9–20Google Scholar
  69. Wu S, He H, Inthapanya X, Yang C, Li L., Zeng G, Han Z (2017) Role of biochar on composting of organic wastes and remediation of contaminated soils—a review. Environ Sci Pollut Res 24:16560–16577Google Scholar
  70. Wu YJ, Zhou H, Zou ZJ, Zhu W, Yang WT, Peng PQ, Zeng M, Liao BH (2016) A three-year in-situ study on the persistence of a combined amendment (lime stone + sepiolite) for remedying paddy soil polluted with heavy metals. Ecotoxicol Environ Saf 130:163–170CrossRefGoogle Scholar
  71. Xiao-Wei H, Huang YZ, Yan-Shan C (2010) Effects of red mud addition on fractionation and bio-accessibility of Pb and Zn in contaminated soil. China J Environ Eng 4:1431–1435Google Scholar
  72. Xuexiu C, Xiaodong S (2001) Heavy metal pollution and food security. China J Yunnan Environ Sci 4:21–24Google Scholar
  73. Yang X, Lu K, McGrouther K, Che L, Hu G, Wang Q, Liu X, Shen L, Huang H, Ye Z, Wang H (2017) Bioavailability of Cd and Zn in soils treated with biochars derived from tobacco stalk and dead pigs. J Soils Sediments 17:751–762CrossRefGoogle Scholar
  74. Zhang WH, Huang H, Tan FF, Wang H, Qiu RL (2010) Influence of EDTA washing on the species and mobility of heavy metals residual in soils. J Hazard Mater 173:369–376CrossRefGoogle Scholar
  75. Zhang ZH, Solaiman ZM, Meney K, Murphy DV, Rengel Z (2013) Biochars immobilize soil cadmium, but do not improve growth of emergent wetland species Juncus subsecundus in cadmium-contaminated soil. J Soils Sediments 13:140–151CrossRefGoogle Scholar
  76. Zhang RH, Li ZG, Liu XD, Wang BC, Zhou GL, Huang XX, Lin CF, Wang AH, Brooks M (2017) Immobilization and bioavailability of heavy metals in greenhouse soils amended with rice straw-derived biochar. Ecol Eng 98:183–188CrossRefGoogle Scholar
  77. Zhou H, Zhou X, Zeng M, Liao BH, Liu L, Yang WT, Wu YM, Qiu QY, Wang YJ (2014) Effects of combined amendments on heavy metal accumulation in rice (Oryza sativa L.) planted on contaminated paddy soil. Ecotoxicol Environ Saf 101:226–232CrossRefGoogle Scholar
  78. Zhou R, Liu X, Luo L, Zhou Y, Wei J, Chen A, Tang L, Wu H, Zhang F, Wang Y (2017) Remediation of Cu, Pb, Zn and Cd-contaminated agricultural soil using a combined red mud and compost amendment. Inter Biodet Biodeg 118:73–81CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Mukesh Kumar Awasthi
    • 1
    Email author
  • Quan Wang
    • 1
  • Hongyu Chen
    • 1
  • Tao Liu
    • 1
  • Sanjeev Kumar Awasthi
    • 1
  • Yumin Duan
    • 1
  • Sunita Varjani
    • 2
  • Ashok Pandey
    • 3
  • Zengqiang Zhang
    • 1
    Email author
  1. 1.College of Natural Resources and EnvironmentNorthwest A&F UniversityYanglingPeople’s Republic of China
  2. 2.Gujarat Pollution Control BoardGandhinagarIndia
  3. 3.CSIR-Indian Institute of Toxicology ResearchLucknowIndia

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