Effect of biogas slurry addition on soil properties, yields, and bacterial composition in the rice-rape rotation ecosystem over 3 years
Organic treatments may improve soil nutrient availability and ecological functions. This study aimed to determine the effect of biogas slurry (BS) on soil properties, yields, and bacterial community activity and to examine the main environmental factors impacting bacterial compositions in the ecosystem.
Materials and methods
A 3-year field experiment was conducted in yellow soil under a rice-rape rotation to understand the effect of BS originating from anaerobically digested pig waste on soil chemical and bacterial compositions.
Results and discussion
Compared with an inorganic nutrient treatment and a control, adding BS at a moderate dose (BS6, 165.1 t ha−1) positively affected the rice and rape yields, soil fertility, and bacterial diversity. The BS was more suitable than chemical fertilizer for maintaining agricultural soil sustainability, especially the BS6 and BS7 treatments (165.1 and 182.1 t ha−1, respectively), by improving nutrient content, increasing soil pH, and promoting soil crumb structure formation. The relative abundance of Actinobacteria in BS6 was decreased by 37.9% compared with the untreated soil and 27.7% compared with the fertilizer treatment, while the relative abundance of Nitrospirae in BS6 increased by 41.2% and 43.5% compared with these treatments, respectively. Redundancy analyses (RDA) showed that pH, rape yield, cation exchange capacity (CEC), and total nitrogen (TN) were significantly correlated with soil bacterial community composition and explained 56.0%, 46.4%, 43.2%, and 34.9% of the total community variability, respectively. The soil bacterial diversity depended mainly on soil pH, and crop rotation played an important role in changing the bacterial community.
The soil bacterial composition was clearly altered after 3 years of BS treatments. These increases in bacterial diversity could be of ecological significance in maintaining soil fertility and functionality.
KeywordsBacterial composition Biogas slurry Pig waste Rice-rape rotation
This work was supported by the Provincial Science and Technology Support Program of Sichuan (2014NZ0045, 2016NZ0039).
- Bao S (2008) Soil agricultural chemistry analysis. China Agriculture Press, BeijingGoogle Scholar
- Bharde NM, Shivay YS, Singh S (2003) Effect of biogas slurry and neem oil-treated urea sources on rice (Oryza sativa)-wheat (Triticum aestivum) cropping system. Indian J Agron 48:73–77Google Scholar
- Gu YF et al (2017) Long-term fertilization structures bacterial and archaeal communities along soil depth gradient in a paddy soil. Front Microbiol 8: 1–15Google Scholar
- Kuffner M, Hai B, Rattei T, Melodelima C, Schloter M, Zechmeister-Boltenstern S, Jandl R, Schindlbacher A, Sessitsch A (2012) Effects of season and experimental warming on the bacterial community in a temperate mountain forest soil assessed by 16 S r RNA gene pyrosequencing. FEMS Microbiol Ecol 82:551–562CrossRefGoogle Scholar
- Ladha JK et al (2004) Long-term effects, of organic inputs on yield and soil fertility in the rice-wheat rotation. Soil Sci Soc Am J 68:845–853Google Scholar
- Shankarappa TH, Gurumurthy SB, Patil SV, Lokesh MS (2012) Influence of phosphate enriched biogas spent slurry (BSS) on growth and yield of sunflower (Helianthus annuus). Int J Plant Sci 3:253–258Google Scholar
- Yan-li Z (2012) Effects of application of biogas slurry of pig dung on physical and chemical properties of soil. J Anhui Agric Sci 40:15202–15203,15213Google Scholar
- Yi-qi W, Wen-biao H, Yu-zhu Z (2016) Effects of biogas slurry on soil physical and chemical properties. J Anhui Agric Sci 44:193–195Google Scholar
- Zhao J et al (2014) Responses of bacterial communities in arable soils in a rice wheat cropping system to different fertilizer regimes and sampling times. PLoS One 9:1–10Google Scholar
- Zheng J, Chen J, Pan G, Liu X, Zhang X, Li L, Bian R, Cheng K, Jinwei Z (2016) Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from southwest China. Sci Total Environ 571:206–217CrossRefGoogle Scholar