Journal of Soils and Sediments

, Volume 19, Issue 1, pp 255–262 | Cite as

Decreased soil substrate availability with incubation time weakens the response of microbial respiration to high temperature in an alpine meadow on the Tibetan Plateau

  • Hanke Liu
  • Wangwang Lv
  • Shiping WangEmail author
  • Caiyun Luo
  • Zhenhua Zhang
  • Zhezhen Wang
  • Lili Jiang
  • Xiaoyong Cui
Soils, Sec 3 • Remediation and Management of Contaminated or Degraded Lands • Research Article



Increased microbial respiration (Rm) usually decreases with warming time in incubation and field manipulative experiments, and substrate depletion and/or microbial thermal acclimation to warming is hypothesized to be responsible for this decrease. However, few studies have been conducted to determine the mechanism for this decrease, especially in alpine regions.

Materials and methods

First, soils taken from an alpine meadow on the Tibetan Plateau were incubated at three different temperatures (i.e., 5, 15, and 25 °C) with two soil moistures (i.e., 30 and 60% water holding capacity (WHC)) for 58 days, then another two experiments were conducted with incubation at high-low-high temperature (i.e., 25–15–25 °C) for 2 weeks and glucose-induced respiration (GIR) for 4 h.

Results and discussion

Rm increased with soil temperature increase at 60% WHC, but there were no significant differences between incubation at 5 and 15 °C with 30% WHC. Higher Rm was found at 60% WHC than at 30% WHC only when incubated at 15 and 25 °C. Both the high-low-high temperature incubation and GIR experiments indicated that decreased soil substrate availability weakened the responses of Rm to high temperature at 60% WHC, and there was no microbial thermal acclimation to temperature over the incubation period. Temperature sensitivity of Rm (Q10) was not affected by soil moisture, high-low-high temperature incubation, or glucose addition.


Our findings suggest that the increase in Rm at higher temperature disappeared over the incubation period, and that this could be due to depletion of soil substrate availability.


Alpine meadow Incubation Microbial respiration Soil moisture Soil temperature Substrate addition Tibetan Plateau 



Authors are grateful for Dr. Andreas Wilkes for English language editing.

Funding information

This work was supported by funding from the Key Project of the Chinese Academy of Sciences (KFZD-SW-312), the National Science Foundation of China (41731175, 31672470), and the National Key Research and Development Program of China (2016YFC0501801).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hanke Liu
    • 1
  • Wangwang Lv
    • 1
    • 2
  • Shiping Wang
    • 2
    • 3
    Email author
  • Caiyun Luo
    • 4
  • Zhenhua Zhang
    • 4
  • Zhezhen Wang
    • 5
  • Lili Jiang
    • 2
  • Xiaoyong Cui
    • 1
  1. 1.University of the Chinese Academy of SciencesBeijingChina
  2. 2.Key Laboratory of Alpine Ecology and BiodiversityInstitute of Tibetan Plateau Research, Chinese Academy of SciencesBeijingChina
  3. 3.CAS Center for Excellence of Tibetan Plateau Earth ScienceChinese Academy of SciencesBeijingChina
  4. 4.Key Laboratory of Adaptation and Evolution of Plateau BiotaNorthwest Institute of Plateau Biology, Chinese Academy of SciencesXiningChina
  5. 5.University of Chicago Medicine and Biological Sciences DivisionChicagoUSA

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