Ellagic acid prolongs the lifespan of Drosophila melanogaster
Wild-type Canton-S flies of Drosophila melanogaster were treated with ellagic acid at 100 μM and 200 μM concentrations. Longevity assay showed male flies fed with 200 μM ellagic acid displayed longer mean lifespan and maximum lifespan than control flies. Female flies fed with 200 μM ellagic acid laid less number of eggs than control. The eclosion time was less in female flies fed with 200 μM ellagic acid. Ellagic acid fed female flies performed better than male flies and control flies for heat shock tolerance and starvation stress. Male flies treated with 100 μM ellagic acid recovered faster from cold shock compared with control flies. Male and female flies treated with ellagic acid displayed increased survival following exposure to 5% hydrogen peroxide. Gene expression studies displayed upregulated expressions of CAT, dFOXO, ATG1, and SOD2 in ellagic acid–treated male flies, and upregulated expressions of dFOXO, CAT, and SOD2 in ellagic acid–treated female flies. Results from these studies show the pro-longevity effect of ellagic acid on Drosophila melanogaster.
KeywordsEllagic acid Drosophila melanogaster Longevity Stress Gene expression
We thank Dr. N.B. Ramachandra at Mysore University, National Drosophila Stock Centre, India, for providing the wild-type Canton-S fly strain. We are thankful to Vellore Institute of Technology for the seed grant and facilities provided.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Baeeri M, Momtaz S, Navaei-Nigjeh M, Niaz K, Rahimifard M, Ghasemi-Niri SF, Sanadgol N, Hodjat M, Sharifzadeh M, Abdollahi M (2017) Molecular evidence on the protective effect of ellagic acid on phosalone-induced senescence in rat embryonic fibroblast cells. Food Chem Toxicol. https://doi.org/10.1016/j.fct.2016.12.008 CrossRefGoogle Scholar
- Baeeri M, Mohammadi-Nejad S, Rahimifard M, Navaei-Nigjeh M, Moeini-Nodeh S, Khorasani R, Abdollahi M (2018) Molecular and biochemical evidence on the protective role of ellagic acid and silybin against oxidative stress-induced cellular aging. Mol Cell Biochem. https://doi.org/10.1007/s11010-017-3172-0 CrossRefGoogle Scholar
- Balasubramani SP, Mohan J, Chatterjee A, Patnaik E, Kukkupuni SK, Nongthomba U, Venkatasubramanian P (2014) Pomegranate juice enhances healthy lifespan in Drosophila melanogaster: an exploratory study. Front Public Health. https://doi.org/10.3389/fpubh.2014.00245
- Han DH, Lee MJ, Kim JH (2006) Antioxidant and apoptosis-inducing activities of ellagic acid. Anticancer ResGoogle Scholar
- Han SK, Lee D, Lee H, Kim D, Son HG, Yang J-S, Lee S-JV, Kim S (2016) OASIS 2: online application for survival analysis 2 with features for the analysis of maximal lifespan and healthspan in aging research. Oncotarget. https://doi.org/10.18632/oncotarget.11269
- Lashmanova E, Zemskaya N, Proshkina E, Kudryavtseva A, Volosnikova M, Marusich E, Leonov S, Zhavoronkov A, Moskalev A (2017) The evaluation of geroprotective effects of selected flavonoids in Drosophila melanogaster and Caenorhabditis elegans. Front Pharmacol 8. https://doi.org/10.3389/fphar.2017.00884
- Linford NJ, Bilgir C, Ro J, Pletcher SD (2013) Measurement of lifespan in Drosophila melanogaster. J Vis Exp. https://doi.org/10.3791/50068
- Macedo GE, Gomes KK, Rodrigues NR, Martins IK, Wallau GDL, Carvalho NR, Cruz LCD, Costa Silva DGD, Boligon AA, Franco JL, Posser T (2017) Senecio brasiliensis impairs eclosion rate and induces apoptotic cell death in larvae of Drosophila melanogaster. Comp Biochem Physiol Part - C Toxicol Pharmacol. https://doi.org/10.1016/j.cbpc.2017.05.004 Google Scholar
- Pomatto LCD, Davies KJA (2018) Adaptive homeostasis and the free radical theory of ageing. Free Radic Biol Med. https://doi.org/10.1016/j.freeradbiomed.2018.06.016 CrossRefGoogle Scholar
- Sanadgol N, Golab F, Tashakkor Z, Taki N, Kouchi SM, Mostafaie A, Mehdizadeh M, Abdollahi M, Taghizadeh G, Sharifzadeh M (2017) Neuroprotective effects of ellagic acid on cuprizone-induced acute demyelination through limitation of microgliosis, adjustment of CXCL12/IL-17/IL-11 axis and restriction of mature oligodendrocytes apoptosis. Pharm Biol. https://doi.org/10.1080/13880209.2017.1319867 CrossRefGoogle Scholar
- Sepand MR, Ghahremani MH, Razavi-Azarkhiavi K, Aghsami M, Rajabi J, Keshavarz-Bahaghighat H, Soodi M (2016) Ellagic acid confers protection against gentamicin-induced oxidative damage, mitochondrial dysfunction and apoptosis-related nephrotoxicity. J Pharm Pharmacol. https://doi.org/10.1111/jphp.12589 CrossRefGoogle Scholar
- Staats S, Wagner AE, Kowalewski B, Rieck FT, Soukup ST, Kulling SE, Rimbach G (2018b) Dietary resveratrol does not affect life span, body composition, stress response, and longevity-related gene expression in Drosophila melanogaster. Int J Mol Sci. https://doi.org/10.3390/ijms19010223 CrossRefGoogle Scholar
- Wang C, Wheeler CT, Alberico T, Sun X, Seeberger J, Laslo M, Spangler E, Kern B, De Cabo R, Zou S (2013) The effect of resveratrol on lifespan depends on both gender and dietary nutrient composition in Drosophila melanogaster. Age (Omaha). https://doi.org/10.1007/s11357-011-9332-3 CrossRefGoogle Scholar