Effect of anolytic nitrite concentration on electricity generation and electron transfer in a dual-chamber microbial fuel cell

  • Rongchang WangEmail author
  • Xuehao Wang
  • Xinyi Zhou
  • Jiabin Yao
Research Article


This study reports the effect of anolytic nitrite concentration on electricity generation and electron transfer in microbial fuel cells (MFCs). Anolytic nitrite enhanced the electricity generation capability of the MFCs at relatively low concentrations (< 60 mg·L−1) but inhibited the activity of anodic electrogenic bacteria at high concentrations. In the anode chamber of the MFC, nitrite was converted to nitrate-releasing electrons before being quickly removed through denitrification. Nitrite alone (in the absence of organic matters) could not perform as an electricity production matrix but promoted electricity production as a co-matrix in the MFC. At an influent nitrite concentration of 60 mg·L−1, the coulombic efficiency of the MFC was minimized at approximately 5.4%, and the charge transfer resistance was also lowest, while the concentrations of extracellular polymeric substances (EPS) and cytochrome c were both maximized. Higher anolytic nitrite concentrations (> 60 mg·L−1) inhibited the production of cytochrome c and EPS and increased the charge transfer resistance, thereby reducing the efficiency of electron transfer in the anodic biofilm. The results provide valuable guidelines for MFC applications in wastewater treatment processes with nitrite-containing influents.


Microbial fuel cell Electricity generation Nitrite removal Extracellular polymeric substances (EPS) Cytochrome c 



This research was supported by the National Key R&D Program of China (2016YFC0400805), the National Natural Science Foundation of China (51878466) and the National Science and Technology Major Project of China on Water Pollution Control and Management (2017ZX07206-001). We also thank the 111 project (B13017) of Tongji University. Dr. Rongchang Wang was supported by the Shanghai Peak Discipline Program at Shanghai Institute of Pollution Control and Ecological Security.

Supplementary material

11356_2019_7323_MOESM1_ESM.doc (249 kb)
ESM 1 (DOC 249 kb)


  1. Al-Mamun A, Baawain MS, Egger F, Al-Muhtaseb AH, Ng HY (2017) Optimization of a baffled-reactor microbial fuel cell using autotrophic denitrifying bio-cathode for removing nitrogen and recovering electrical energy. Biochem Eng J 120:93–102CrossRefGoogle Scholar
  2. APHA LS, Clesceri AE, Greenberg AD (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington, DCGoogle Scholar
  3. Barsoukov E and Macdonald J R (2005) Impedance Spectroscopy: Theory, Experiment, and Applications. Wiley-Interscience, Hoboken.Google Scholar
  4. Borole AP, Aaron D, Hamilton CY, Tsouris C (2010) Understanding long-term changes in microbial fuel cell performance using electrochemical impedance spectroscopy. Environ Sci Technol 44:2740–2745CrossRefGoogle Scholar
  5. Cao B, Shi L, Brown RN, Xiong Y, Fredrickson JK, Romine MF, Marshall MJ, Lipton MS, Beyenal H (2011) Extracellular polymeric substances from Shewanella sp. HRCR-1 biofilms: characterization by infrared spectroscopy and proteomics. Environ Microbiol 13:1018–1031CrossRefGoogle Scholar
  6. Chen H, Zheng P, Zhang J, Xie Z, Ji J, Ghulam A (2014) Substrates and pathway of electricity generation in a nitrification-based microbial fuel cell. Bioresour Technol 161:208–214CrossRefGoogle Scholar
  7. Ding W, Cheng S, Yu L, Huang H (2017) Effective swine wastewater treatment by combining microbial fuel cells with flocculation. Chemosphere 182:567–573CrossRefGoogle Scholar
  8. Fang HH, Xu L, Chan K (2002) Effects of toxic metals and chemicals on biofilm and biocorrosion. Water Res 36:4709–4716CrossRefGoogle Scholar
  9. Faraghi N and Ebrahimi S (2012) Nitrite as a candidate substrate in microbial fuel cells. Biotechnol Lett 34:1483–1486CrossRefGoogle Scholar
  10. Fu Y, Kok RAW, Dankbaar B, Ligthart PEM, Riel ACR (2018) Factors affecting sustainable process technology adoption: a systematic literature review. J Clean Prod 205:226–251CrossRefGoogle Scholar
  11. Gaudy AF (1962) Colorimetric determination of protein and carbohydrate. Ind Water Wastes 7:17–22Google Scholar
  12. Gralnick JA, Newman DK (2007) Extracellular respiration. Mol Microbiol 65:1–11CrossRefGoogle Scholar
  13. Hamilton WA (2003) Microbially influenced corrosion as a model system for the study of metal microbe interactions: a unifying electron transfer hypothesis. Biofouling 19:65–76CrossRefGoogle Scholar
  14. Herdman AT, Stapells DR (2003) Auditory steady-state response thresholds of adults with sensorineural hearing impairments: Umbrales de las respuestas auditivas de estado estable en adultos con hipoacusia sensorineural. Int J Audiol 42:237–248CrossRefGoogle Scholar
  15. Hutchinson AJ, Tokash JC, Logan BE (2011) Analysis of carbon fiber brush loading in anodes on startup and performance of microbial fuel cells. J Power Sources 196:9213–9219CrossRefGoogle Scholar
  16. Katz E, Willner I (2003) Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedimetric immunosensors, DNA-sensors, and enzyme biosensors. Electroanalysis 15:913–947CrossRefGoogle Scholar
  17. Kim Y, Shin S, Chang IS, Moon S (2014) Characterization of uncharged and sulfonated porous poly(vinylidene fluoride) membranes and their performance in microbial fuel cells. J Membr Sci 463:205–214CrossRefGoogle Scholar
  18. Kleerebezem R, van Loosdrecht MC (2007) Mixed culture biotechnology for bioenergy production. Curr Opin Biotechnol 18:207–212CrossRefGoogle Scholar
  19. Logan BE, Regan JM (2006) Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol 14:512–518CrossRefGoogle Scholar
  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  21. Lu H, Oehmen A, Virdis B, Keller J, Yuan Z (2006) Obtaining highly enriched cultures of Candidatus Accumulibacter phosphates through alternating carbon sources. Water Res 40:3838–3848CrossRefGoogle Scholar
  22. Ma B, Yang L, Wang Q, Yuan Z, Wang Y, Peng Y (2017) Inactivation and adaptation of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria when exposed to free nitrous acid. Bioresour Technol 245(Pt A):1266–1270CrossRefGoogle Scholar
  23. Magnussen BF, Hjertager BW (1981) On the structure of turbulence and a generalized eddy dissipation concept for chemical reaction in turbulent flow 19th AIAA aerospace meeting, St. Louis, USAGoogle Scholar
  24. McCarty P L, Bae J, Kim J (2011) Domestic wastewater treatment as a net energy producer-can this be achieved? Environmental Science and Technology, 45(17):7100–7106.CrossRefGoogle Scholar
  25. Moharir PV, Tembhurkar AR (2018) Effect of recirculation on bioelectricity generation using microbial fuel cell with food waste leachate as substrate. Int J Hydrog Energy 43(21):10061–10069CrossRefGoogle Scholar
  26. Mowat CG, Chapman SK (2005) Multi-heme cytochromes—new structures, new chemistry. Dalton Trans 3381–3389Google Scholar
  27. Oh ST, Kim JR, Premier GC, Lee TH, Kim C, Sloan WT (2010) Sustainable wastewater treatment: how might microbial fuel cells contribute. Biotechnol Adv 28:871–881CrossRefGoogle Scholar
  28. O'Toole G, Kaplan HB, Kolter R (2000) Biofilm formation as microbial development. Annu Rev Microbiol 54:49–79CrossRefGoogle Scholar
  29. Park Y, Nguyen VK, Park S, Yu J, Lee T (2018) Effects of anode spacing and flow rate on energy recovery of flat-panel air-cathode microbial fuel cells using domestic wastewater. Bioresour Technol 258:57–63CrossRefGoogle Scholar
  30. Qureshi N, Annous BA, Ezeji TC, Karcher P, Maddox IS (2005) Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates. Microb Cell Factories 4:24CrossRefGoogle Scholar
  31. Sheng G, Yu H, Li X (2010) Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems: a review. Biotechnol Adv 28:882–894CrossRefGoogle Scholar
  32. Sinclair PR, Gorman N, Jacobs JM (2001) Measurement of heme concentration. Curr Protoc Toxicol 8(3):1–8.3.7Google Scholar
  33. Sun G, Thygesen A, Ale MT, Mensah M, Poulsen FW, Meyer AS (2014) The significance of the initiation process parameters and reactor design for maximizing the efficiency of microbial fuel cells. Appl Microbiol Biotechnol 98:2415–2427CrossRefGoogle Scholar
  34. Virdis B, Harnisch F, Batstone DJ, Rabaey K, Donose BC (2012) Non-invasive characterization of electrochemically active microbial biofilms using confocal Raman microscopy. Energy Environ Sci 5:7017–7024CrossRefGoogle Scholar
  35. Virdis B, Rabaey K, Rozendal R A, Yuan Z, Keller J (2010) Simultaneous nitrification, denitrification and carbon removal in microbial fuel cells. Water Research, 2010, 44(9):2970–2980CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institute of Biofilm Technology, Key Laboratory of Yangtze Aquatic Environment (MOE), State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and EngineeringTongji UniversityShanghaiChina

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