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Nitrate level safety to Amazon River shrimp juveniles

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The study’s objective was to evaluate the nitrate level safety for Macrobrachium amazonicum juvenile in the laboratory, a potential native species for culture in Brazil. The experiment consisted of six treatments with six replicates in a completely randomized block design: 0, 250, 500, 1,000, 1,500, and 2,000 mg L−1. Physical and chemical water quality parameters were recorded every 12 h, while the shrimp mortalities in the 24-h interval. Except for nitrate, all physical and chemical water quality parameters remained within the ideal range rearing to this species. No deaths were observed during the first 6 h of exposure range 0-500 mg L−1 concentrations. At 250 mg L−1 N-NO3, the mortality (10%) started from 48 h. At 500 mg L−1 N-NO3, shrimp mortalities occurred after 24 h, reaching 60% after 72 h. In the treatments with 1000 and 1500 mg L−1 N-NO3 concentrations, dead shrimps can be observed after 24 h, with a mortality rate of 78% and 90% of the population in 96 h, respectively. All shrimps exposed at 2000 mg L−1 died in 96 h. The LC50 values obtained decreased with increasing exposure time. Based on LC50 (96 h), the N-NO3 level safety to M. amazonicum is 48.5 mg L−1.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.


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This study was supported by the National Council for Scientific and Technological Development - CNPq (Project no. 444367/2014-4). The authors are grateful for all the support.

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Correspondence to Jô de Farias Lima.

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de Farias Lima, J., Lobo, E.T., Bastos, A.M. et al. Nitrate level safety to Amazon River shrimp juveniles. Environ Sci Pollut Res 27, 4546–4550 (2020).

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  • Macrobrachium amazonicum
  • Nitrate lethal level
  • Palaemonidae
  • Toxic effect