The study’s objective was to evaluate the nitrate level safety for Macrobrachium amazonicum juvenile in the laboratory, a potential native species for culture in Brazil. The experiment consisted of six treatments with six replicates in a completely randomized block design: 0, 250, 500, 1,000, 1,500, and 2,000 mg L−1. Physical and chemical water quality parameters were recorded every 12 h, while the shrimp mortalities in the 24-h interval. Except for nitrate, all physical and chemical water quality parameters remained within the ideal range rearing to this species. No deaths were observed during the first 6 h of exposure range 0-500 mg L−1 concentrations. At 250 mg L−1 N-NO3−, the mortality (10%) started from 48 h. At 500 mg L−1 N-NO3−, shrimp mortalities occurred after 24 h, reaching 60% after 72 h. In the treatments with 1000 and 1500 mg L−1 N-NO3− concentrations, dead shrimps can be observed after 24 h, with a mortality rate of 78% and 90% of the population in 96 h, respectively. All shrimps exposed at 2000 mg L−1 died in 96 h. The LC50 values obtained decreased with increasing exposure time. Based on LC50 (96 h), the N-NO3− level safety to M. amazonicum is 48.5 mg L−1.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
Alonso A, Camargo JA (2003) Short-term toxicity of ammonia, nitrite, and nitrate to the aquatic snail Potamopyrgus antipodarum (Hydrobiidae, Mollusca). Bull Environ Contam Toxicol 70(5):1006–1012. https://doi.org/10.1007/s00128-003-0082-5
Ayres M, Ayres M Jr, Ayres DL, Santos AA (2007) Bioestat 5.3. Aplicações estatísticas nas áreas das ciências biomédicas. Ong Mamiraua, Belém, PA
Camargo JA, Alonso A, Salamanca A (2005) Nitrate toxicity to aquatic animals: a review with new data for freshwater invertebrates. Chemosphere. 58:1255–1267. https://doi.org/10.1016/j.chemosphere.2004.10.044
Campos RC, Filho KCM, D’Incao F, Poersch L, Wasielesky W (2012) Toxicidade aguda da amônia, nitrito e nitrato sobre os juvenis de camarão rosa Farfantepenaeus brasiliensis (latreille, 1817) (Crustacea: Decapoda). Atlântica. 34:75–81. https://doi.org/10.5088/atl%C3%A2ntica.v34i1.2712
Campos BR, Furtado PS, D’Incao F, Poersch L, Wasielesky W (2015) The chronic toxicity of ammonia, nitrite and nitrate on juvenile Farfantepenaeus brasiliensis (Crustacea: Decapoda). Bol Inst Pesca 41:261–269
Cheng SY, Tsai SJ, Chen JC (2002) Accumulation of nitrate in the tissues of Penaeus monodon following elevated ambient nitrate exposure after different time periods. Aquat Toxicol 56(1):133–146. https://doi.org/10.1007/s00244-001-0056-8
Cobo ML, Sonnenholzner S, Wille M, Sorgeloos P (2012) Ammonia tolerance of Litopenaeus vannamei (Boone) larvae. Aquac Res 45(3):470–475. https://doi.org/10.1111/j.1365-2109.2012.03248.x
Dutra FM, Forneck SC, Brazão CC, Freire CA, Ballester ELC (2016) Acute toxicity of ammonia to various life stages of the Amazon River prawn, Macrobrachium amazonicum, Heller, 1862. Aquaculture 453:104–109. https://doi.org/10.1016/j.aquaculture.2015.11.038
Furtado PS, Campos BR, Serra FP, Klosterhoff M, Romano LA, Wasielesky W Jr (2015) Effects of nitrate toxicity in the Pacific white shrimp, Litopenaeus vannamei, reared with biofloc technology (BFT). Aquac Int 23:315–327. https://doi.org/10.1007/s10499-014-9817-z
Hamilton MA, Russo RC, Thurston RV (1977) Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays. Environ Sci Technol 11:714–719 Correction, 12, 417, 1978
Hayd L, Lemos D, Valenti WC (2010) Ontogenetic variation in ammonia excretion during the early life stages of the Amazon River prawn, Macrobrachium amazonicum. J World Aquacult Soc 41:107–115. https://doi.org/10.1111/j.1749-7345.2009.00339.x
Hayd LA, Lemos D, Valenti WC (2014) Effects of ambient nitrite on Amazon River prawn Macrobrachium amazonicum larvae. J World Aquacult Soc 45:55–64. https://doi.org/10.1111/JWAS.12071
Kuhn DD, Smith SA, Boardman GD, Angier MW, Marsh LFJ, George J (2010) Chronic toxicity of nitrate to Pacific white shrimp, Litopenaeus vannamei: impacts on survival, growth, antennae length, and pathology. Aquaculture. 309:109–114. https://doi.org/10.1016/j.aquaculture.2010.09.014
Lima JF, Duarte SS, Bastos AM, Carvalho T (2019) Performance of an aquaponics system using constructed semi-dry wetland with lettuce (Lactuca sativa L.) on treating wastewater of culture of Amazon River shrimp (Macrobrachium amazonicum). Environ Sci Pollut Res 26(13):13476–13488. https://doi.org/10.1007/s11356-019-04496-5
Marques HLA, Moraes-Valenti PMC (2012) Current status and prospects of farming the giant river prawn (Macrobrachium rosembergii (De Man 1879) and the Amazon River prawn Macrobrachium amazonicum (Heller 1862)) in Brazil. Aquac Res 43:984–992. https://doi.org/10.1111/j.1365-2109.2012.03137.x
Marques HLA, Lombardi JV, Mallasen M, Barros HP, Boock MV (2010) Stocking densities in cage rearing of Amazon River prawn (Macrobrachium amazonicum) during nursery phases. Aquaculture. 307:201–205. https://doi.org/10.1016/j.aquaculture.2010.07.035
Moraes-Valenti PMC, Valenti WC (2007) Effect of intensification on grow out of the Amazon River prawn Macrobrachium amazonicum. J World Aquacult Soc 38:516–526. https://doi.org/10.1111/j.1749-7345.2007.00125.x
Moraes-Valenti P, Valenti WC (2010) Culture of the Amazon River prawn Macrobrachium amazonicum. In: New MB, Valenti WC, Tidwell JH, D’ Abramo LR, Kutty MN (eds) Freshwater prawns: biology and farming. Wiley-Blackwell, Oxford, pp 485–501
Muir PR, Sutton DC, Owens L (1991) Nitrate toxicity to Penaeus monodon protozoea. Humphrey- Sydney: Mar Biol 108:67–71
Poersch LH, Santos MHS, Wasielesky W (2007) Efeito agudo do nitrato sobre alevinos da tainha Mugil platanus (Pisces: Mugilidae). Bol Inst Pesca 33:247–252
Preto BL, Pizzato GM, Valenti WC (2008) Uso de bandejas de alimentação na fase de engorda do camarão-da-amazônia, Macrobrachium amazonicum (Heller, 1862). Bol Inst Pesca 34:125–130
Romano N, Zeng C (2007) Effects of potassium on nitrate mediated changes to osmoregulation in marine crabs. Aquat Toxicol 85:202–208. https://doi.org/10.1016/j.aquatox.2007.09.004
Romano N, Zeng C (2009) Evaluating the newly proposed protocol of incorporated potassium in nitrate toxicity experiments at different salinities: a case study with the tiger prawn, Penaeus monodon, juveniles. Aquaculure. 298:304–309. https://doi.org/10.1016/j.aquaculture.2009.01.035
Romano N, Zeng C (2013) Toxic effects of ammonia, nitrite and nitrate to decapods crustaceans: a review on factors influencing their toxicity, physiological consequences and coping mechanisms. Rev Fish Sci 21:1–21. https://doi.org/10.1080/10641262.2012.753404
Song P, Wu L, Guan W (2015) Dietary nitrates, nitrites, and nitrosamines intake and the risk of gastric cancer: a meta-analysis. Nutrients 7:9872–9895. https://doi.org/10.3390/nu7125505
Tsai SJ, Chen JC (2002) Acute toxicity of nitrate on Penaeus monodon juveniles at different salinity levels. Aquaculture. 213:163–170
Vanwyk P, Scarpa J (1999) Water quality requirements and management. In: Farming marine shrimping recirculating freshwater systems (ed. by P. Van Wyk), pp.128-138. Florida Department of Agriculture and Consumer Services, Tallahassee, FL, USA.
Wasielesky WJ, Poersch LH, Martins TG, Miranda-Filho KC (2017) Chronic effects of nitrogenous compounds on survival and growth of juvenile pink shrimp. Braz J Biol 77(3):558–565. https://doi.org/10.1590/1519-6984.18415
Wolfe AH, Patz JA (2002) Reactive nitrogen and human health: acute and long-term implications. AMBIO: A Journal of the Human Environment 31:120–125
This study was supported by the National Council for Scientific and Technological Development - CNPq (Project no. 444367/2014-4). The authors are grateful for all the support.
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de Farias Lima, J., Lobo, E.T., Bastos, A.M. et al. Nitrate level safety to Amazon River shrimp juveniles. Environ Sci Pollut Res 27, 4546–4550 (2020). https://doi.org/10.1007/s11356-019-07033-6
- Macrobrachium amazonicum
- Nitrate lethal level
- Toxic effect