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Biochemical and molecular characterization of arsenic response from Azospirillum brasilense Cd, a bacterial strain used as plant inoculant

  • Mariana Elisa VezzaEmail author
  • Maria Florencia Olmos Nicotra
  • Elizabeth Agostini
  • Melina Andrea Talano
Research Article
  • 29 Downloads

Abstract

Azospirillum brasilense Cd is a bacterial strain widely used as an inoculant of several crops due to its plant growth promoting properties. However, its beneficial effects depend on its viability and functionality under adverse environmental conditions, including the presence of arsenic (As) in agricultural soils. Therefore, the aim of this work was to evaluate the response of A. brasilense Cd to arsenate (AsV) and arsenite (AsIII). This bacterium was tolerant to As concentrations frequently found in soils. Moreover, properties related to roots colonization (motility, biofilm, and exopolymers) and plant growth promotion (auxin, siderophore production, and N2 fixation) were not significantly affected by the metalloid. In order to deepen the understanding on As responses of A. brasilense Cd, As resistance genes were sequenced and characterized for the first time in this work. These genes could mediate the redox As transformation and its extrusion outside the cell, so they could have direct association with the As tolerance observed. In addition, its As oxidation/reduction capacity could contribute to change the AsV/AsIII ratio in the environment. In conclusion, the results allowed to elucidate the As response of A. brasilense Cd and generate interest for its potential use in polluted environments.

Keywords

Azospirillum brasilense Cd Arsenic Biofilm Exopolymers ars operon Arsenic transformation 

Notes

Acknowledgments

Mariana Elisa Vezza is a CONICET scholarship. Maria Florencia Olmos Nicotra is a postdoctoral fellow. Elizabeth Agostini and Melina Andrea Talano are members of the research career from CONICET. We wish to thank FONCYT (PICT 828/13), SeCyT-UNRC (PPI-C439), and CONICET.

Supplementary material

11356_2019_6959_MOESM1_ESM.pdf (631 kb)
ESM 1 (PDF 630 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Biología Molecular, FCEFQyNUniversidad Nacional de Río Cuarto (UNRC)Río CuartoArgentina
  2. 2.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina

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