Antioxidant-oxidant balance and vital parameter alterations in an eukaryotic system induced by aflatoxin B2 exposure

  • Kültiğin ÇavuşoğluEmail author
  • Emine Yalçin
Research Article


This study was performed to evaluate the toxic effects of aflatoxin B2 (AFB2) on antioxidant-oxidant balance and vital parameters such as physiological, cytogenetic, and anatomical alterations in Allium cepa L. root tip cells. Toxic effects of AFB2 on vital parameters were investigated by using the changes in weight gain, germination percentage, chromosomal aberrations (CAs), mitotic index (MI), micronucleus frequency (MN), and anatomical structure. Malondialdehyde (MDA) and reduced glutathion (GSH) levels and superoxide dismutase (SOD) and catalase (CAT) activities in root cells were investigated as antioxidant-oxidant parameters. For this aim, A. cepa bulbs were seperated into five groups as negative control, positive control, and AFB2 treatment groups. In results, while the rate of germination percentage, weight gain, and MI rates decreased, MN and CA frequency increased in AFB2-treated groups compared with the negative control. Most common CAs observed in AFB2-treated groups were fragment and chromosome bridges. It was determined that in 160 μg L−1 AFB2-treated group there was a 70.8% increase in MDA and a 78.1% decrease in GSH level compared with the negative control group and these changes indicate oxidative damage. In 160 μg L−1 AFB2 treatment group, SOD and CAT activities decreased importantly due to inhibition. In anatomical examinations, it was determined that AFB2 treatment caused some anatomical damages in A. cepa root cells such as necrosis, cell deformation, and thickening in cell wall. This study showed that AFB2, which has the least data among aflatoxins, causes serious in vivo toxic effects in A. cepa root cells.


Allium test Aflatoxin B2 Genotoxicity Antioxidant parameters Malondialdehyde Germination 



Aflatoxin B2


Chromosomal aberrations


Mitotic index


Micronucleus frequency




Reduced glutathion


Superoxide dismutase





  1. Aguilar F, Hussain SP, Cerutti P (1993) Aflatoxin B1 induces the transversion of G-T in codon 249 of the p53 tumor suppressor gene in human hepatocytes. Proc Natl Acad Sci 90:8586–8590CrossRefGoogle Scholar
  2. Antonsie-Wiez D (1990) Analysis of the cell cycle in the root meristem of Allium cepa under infl uence of Leda krin. Folia Histochem Cytobiol 26:79–96Google Scholar
  3. Baker AJM (1981) Accumulators and excluders-strategies in the response of plants to heavy metals. J Plant Nutr 3:643–654CrossRefGoogle Scholar
  4. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefGoogle Scholar
  5. Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140Google Scholar
  6. Black HS, Jirgensons B (1967) Interactions of aflatoxin with histones and DNA. Plant Physiol 42:731CrossRefGoogle Scholar
  7. Butler WH, Greenblut M, Lijinsky W (1969) Carcinogenesis in rats by aflatoxins B1, G1 and B2. Cancer Res 29:2206–2211Google Scholar
  8. Chang SB, Kader MA, Wick EL, Wogan GN (1963) Aflatoxin B2: chemical identity and biological activity. Science 142:1191–1192CrossRefGoogle Scholar
  9. Crisan EV (1973) Effects of aflatoxin on seeding growth and ultrastructure in plants. Appl Microbiol 26:991–1000Google Scholar
  10. Fenech M, Chang WP, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E (2003) HUMN Project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 534:65–75Google Scholar
  11. Firbas P, Amon T (2013) Allium chromosome aberration test for evaluation effect of cleaning municipal water with constructed wetland (CW) in Sveti Tomaž, Slovenia. J Bioremediat Biodegrad 4:189–193CrossRefGoogle Scholar
  12. IARC (1993) Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxin. In: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, Lyon, France, vol 56, pp 165-231Google Scholar
  13. Iqbal SZ, Nisar S, Asi MR, Jinap S (2014) Natural incidence of aflatoxins, ochratoxin A and zearalenone in chicken meat and eggs. Food Control 43:98–103CrossRefGoogle Scholar
  14. Jackson PE, Kuang SY, Wang JB, Strickland PT, Muñoz A, Kensler TW, Qian GS, Groopman JD (2003) Prospective detection of codon 249 mutations in plasma of hepatocellular carcinoma patients. Carcinogenesis 24:1657–1663CrossRefGoogle Scholar
  15. Jones HC, Chancey JC, Morton WA, Dashek WV, Llewellyn GC (1980) Toxic responses of germinating pollen and soybeans to aflatoxins. Mycopathologia 72:67–73CrossRefGoogle Scholar
  16. Key JL, Ingle J (1964) Requirement for the synthesis of DNA-like RNA for growth of excised plant tissue. Proc Natl Acad Sci 52:1382–1388CrossRefGoogle Scholar
  17. Leme DM, Marin-Morales MA (2009) Allium cepa test in environmental monitoring: a review on its application. Mutat Res Rev Mutat 682:71–81CrossRefGoogle Scholar
  18. Lilly LJ (1965) Induction of chromosome aberrations by aflatoxin. Nature 207:433–434CrossRefGoogle Scholar
  19. Liu HY, Liao BH, Lu SQ (2004) Toxicity of surfactant, acid rain and Cd2 combined pollution to the nucleus of Vicia faba root tip cells. Chin J Plant Ecol 15:493–496Google Scholar
  20. Maragos CM, Pohland AE (2001) Measurement of aflatoxins using capillary electrophoresis. In: Mycotoxin protocols, 1st edn, Totowa, pp 51–58Google Scholar
  21. Reiss J (1971) Inhibition of germination of cress (Lepidium sativum) by aflatoxin B, and rubratoxin B. Biochem Physiol Pflanz 162:363–367CrossRefGoogle Scholar
  22. Sedlak J, Lindsay RH (1968) Estimation of total protein-bound and non-protein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25:192–205CrossRefGoogle Scholar
  23. Sinha KK, Sinha AK (1993) Effect of aflatoxin B1 on germination index and seedling growth in wheat varieties. Mycopathologia 123:165–169CrossRefGoogle Scholar
  24. Sporn MB, Dingman CW, Phelps HL, Wogan GN (1966) Aflatoxin B1: binding to DNA in vitro and alteration of RNA metabolism in vivo. Science 151:1539–1541CrossRefGoogle Scholar
  25. Staykova TA, Ivanova EN, Velcheva IG (2005) Cytogenetic effect of heavy metal and cyanide in contamined waters from the region of Southwest Bulgaria. J Cell Mol Biol 4:41–46Google Scholar
  26. Tripathi RK, Misra RS (1981) Effect of aflatoxin B1 on chromatin-bound ribonucleic acid polymerase and nucleic acid and protein synthesis in germinating maize seeds. Appl Environ Microbiol 42:389–393Google Scholar
  27. Unyayar S, Celik A, Cekic FO, Gozel A (2006) Cadmium-induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba. Mutagenesis 21:77–81CrossRefGoogle Scholar

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Science and ArtGiresun UniversityGiresunTurkey

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