DNA damage in kidney and parenchymal and non-parenchymal liver cells of adult Wistar rats after subchronic oral treatment with tembotrione

  • Vilena KašubaEmail author
  • Vedran Micek
  • Alica Pizent
  • Blanka Tariba Lovaković
  • Davor Želježić
  • Mirta Milić
  • Nevenka Kopjar
Research Article


DNA damage in the liver and kidney cells of adult male Wistar rats was studied using the comet assay after a 28-day oral administration of tembotrione at doses of 0.0007, 0.0013 and 0.7 mg/kg b.w./day [AOEL (acceptable operator exposure level), REL (residual exposure level) and 1000× AOEL]. As a descriptor of DNA damage, tail intensity was used. Antioxidant status was assessed by activity of glutathione peroxidase (GPx). Significant DNA damage was recorded in the kidney cells at all three doses as compared to negative control. In parenchymal liver cells, significant DNA damage was observed in AOEL and 1000× AOEL doses, while in non-parenchymal liver cells, only AOEL-treated group was significantly different compared to negative control. In both types of liver cells, REL and 1000× AOEL doses were significantly different from the AOEL dose. No significant changes in GPx activity compared to control were observed at any exposure level. The results of the present study suggest that repeated in vivo exposure to tembotrione led to low-level DNA instability in kidney and liver cells. Exposure to the highest tembotrione dose showed a relatively weak response with the alkaline comet assay. Further research should focus on the effects of this herbicide in other models along with different exposure scenarios.


DNA damage Parenchymal and non-parenchymal liver cells Kidney cells Tembotrione 


Funding information

This work was financially supported by the project Organic Pollutants in Environment – Markers and Biomarkers of Toxicity (OPENTOX), funded by the Croatian Science Foundation (grant number 8366).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Vilena Kašuba
    • 1
    Email author
  • Vedran Micek
    • 2
  • Alica Pizent
    • 3
  • Blanka Tariba Lovaković
    • 3
  • Davor Želježić
    • 1
  • Mirta Milić
    • 1
  • Nevenka Kopjar
    • 1
  1. 1.Mutagenesis UnitInstitute for Medical Research and Occupational HealthZagrebCroatia
  2. 2.Animal Breeding UnitInstitute for Medical Research and Occupational HealthZagrebCroatia
  3. 3.Analytical Toxicology and Mineral Metabolism UnitInstitute for Medical Research and Occupational HealthZagrebCroatia

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