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Toxicity of cadmium selenide nanoparticles on the green microalga Chlorella vulgaris : inducing antioxidative defense response

  • Ali MovafeghiEmail author
  • Alireza Khataee
  • Arezoo Rezaee
  • Morteza Kosari-Nasab
  • Roshanak Tarrahi
Research Article
  • 51 Downloads

Abstract

Green algae are dominant primary producers in aquatic environments. Thus, assessing the influences of pollutants such as nanoparticles on the algae is of high ecological significance. In the current study, cadmium selenide nanoparticles (CdSe NPs) were synthesized using the hydrothermal method and their characteristics were determined by X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FT-IR) techniques. Subsequently, the toxicity of synthesized nanoparticles on the green microalga Chlorella vulgaris was investigated. The observations by SEM confirmed that exposure to CdSe NPs had severe impacts on the algal morphology. Furthermore, the obtained results revealed the toxic effect of CdSe NPs by a decrease in the number of cells. Measurement of antioxidant enzymes activity showed an increase in the activity of catalase, and a decrease in the activity of superoxide dismutase (SOD) at high concentrations of CdSe NPs. The exposure of C. vulgaris to CdSe NPs resulted also in a change in algal pigments as well as total phenol content. Taken together, CdSe NPs appeared to have significant cytotoxic effects on C. vulgaris in the applied concentrations.

Keywords

CdSe nanoparticles Chlorella vulgaris Toxicity Antioxidant system 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

References

  1. Arora A, Byrem TM, Nair MG, Strasburg GM (2000) Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Arch Biochem Biophys 373:102–109CrossRefGoogle Scholar
  2. Barceló I, Lana-Villarreal T, Gómez R (2011) Efficient sensitization of ZnO nanoporous films with CdSe QDs grown by successive ionic layer adsorption and reaction (SILAR). J Photochem Photobiol A Chem 220:47–53CrossRefGoogle Scholar
  3. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefGoogle Scholar
  4. Benavides MP, Gallego SM, Tomaro ML (2005) Cadmium toxicity in plants. Braz J Plant Physiol 17:21–34CrossRefGoogle Scholar
  5. Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194Google Scholar
  6. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle Scholar
  7. Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018CrossRefGoogle Scholar
  8. Chance B, Maehly A (1955) Assay of catalases and peroxidases. Method Enzymol 2:764–775CrossRefGoogle Scholar
  9. Cheng J, Qiu H, Chang Z, Jiang Z, Yin W (2016) The effect of cadmium on the growth and antioxidant response for freshwater algae Chlorella vulgaris. Springerplus. 5:1290CrossRefGoogle Scholar
  10. Cui D, Zhang P, Ma Y, He X, Li Y, Zhang J, Zhao Y, Zhang Z (2014) Effect of cerium oxide nanoparticles on asparagus lettuce cultured in an agar medium. Environ Sci Nano 1:459–465CrossRefGoogle Scholar
  11. Dai LP, Xiong ZT, Huang Y, Li MJ (2006) Cadmium-induced changes in pigments, total phenolics, and phenylalanine ammonia-lyase activity in fronds of Azolla imbricata. Environ Toxicol 21:505–512CrossRefGoogle Scholar
  12. Daughton CG (2004) Non-regulated water contaminants: emerging research. Environ Impact Asses 24:711–732CrossRefGoogle Scholar
  13. Duan K, Cui M, Wu Y, Huang X, Xue A, Deng X, Luo L (2018) Effect of dibutyl phthalate on tolerance and lipid accumulation in the green microalgae Chlorella vulgaris. Bull Environ Contam Toxicol 101:338–343CrossRefGoogle Scholar
  14. Dubey A, Goswami M, Yadav K, Chaudhary D (2015) Oxidative stress and nano-toxicity induced by TiO2 and ZnO on WAG cell line. PLos One. 10:e0127493CrossRefGoogle Scholar
  15. Fazelian N, Movafeghi A, Yousefzadi M, Rahimzadeh M (2019) Cytotoxic impacts of CuO nanoparticles on the marine microalga Nannochloropsis oculata. Environ Sci Pollut Res 26:17499–17511CrossRefGoogle Scholar
  16. Khataee A, Hosseini M, Hanifehpour Y, Safarpour M, Joo S (2014) Hydrothermal synthesis and characterization of Nd-doped ZnSe nanoparticles with enhanced visible light photocatalytic activity. Res Chem Intermed 40:495–508CrossRefGoogle Scholar
  17. Khataee A, Movafeghi A, Mojaver N, Vafaei F, Tarrahi R, Dadpour MR (2017) Toxicity of copper oxide nanoparticles on Spirodela polyrrhiza: assessing physiological parameters. Res Chem Intermed 43:927–941CrossRefGoogle Scholar
  18. Klaine SJ, Alvarez PJ, Batley GE, Fernandes TF, Handy RD, Lyon DY, Mahendra S, McLaughlin MJ, Lead JR (2008) Nanomaterials in the environment: behavior, fate, bioavailability, and effects. Environ Toxicol Chem 27:1825–1851CrossRefGoogle Scholar
  19. Kloepfer J, Mielke R, Nadeau J (2005) Uptake of CdSe and CdSe/ZnS quantum dots into bacteria via purine-dependent mechanisms. Appl Environ Microbiol 71:2548–2557CrossRefGoogle Scholar
  20. Lesser MP (2006) Oxidative stress in marine environments: Biochemistry and physiological ecology. Annu Rev Physiol 68:253–278CrossRefGoogle Scholar
  21. Li F, Liang Z, Zheng X, Zhao W, Wu M, Wang Z (2015) Toxicity of nano-TiO2 on algae and the site of reactive oxygen species production. Aquat Toxicol 158:1–13CrossRefGoogle Scholar
  22. Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol 148:350–382CrossRefGoogle Scholar
  23. Ma C, Huangfu X, He Q, Ma J, Huang R (2018) Deposition of engineered nanoparticles (ENPs) on surfaces in aquatic systems: a review of interaction forces, experimental approaches, and influencing factors. Environ Sci Pollut Res 25:33056–33081CrossRefGoogle Scholar
  24. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91:571–577CrossRefGoogle Scholar
  25. Melegari SP, Perreault F, Costa RHR, Popovic R, Matias WG (2013) Evaluation of toxicity and oxidative stress induced by copper oxide nanoparticles in the green alga Chlamydomonas reinhardtii. Aquat Toxicol 142:431–440CrossRefGoogle Scholar
  26. Meng Z-D, Zhu L, Oh W-C (2012) Preparation and high visible-light-induced photocatalytic activity of CdSe and CdSe-C60 nanoparticles. J Ind Eng Chem 18:2004–2009CrossRefGoogle Scholar
  27. Morelli E, Cioni P, Posarelli M, Gabellieri E (2012) Chemical stability of CdSe quantum dots in seawater and their effects on a marine microalga. Aquat Toxicol 122:153–162CrossRefGoogle Scholar
  28. Movafeghi A, Khataee A, Abedi M, Tarrahi R, Dadpour M, Vafaei F (2018) Effects of TiO2 nanoparticles on the aquatic plant Spirodela polyrrhiza: Evaluation of growth parameters, pigment contents and antioxidant enzyme activities. J Environ Sci 64:130–138CrossRefGoogle Scholar
  29. Murray JR, Hackett WP (1991) Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L. Plant Physiol 97:343–351CrossRefGoogle Scholar
  30. Nazari F, Movafeghi A, Jafarirad S, Kosari-Nasab M, Divband B (2018) Synthesis of reduced graphene oxide-silver nanocomposites and assessing their toxicity on the green microalga Chlorella vulgaris. Bionanoscience 8:997–1007CrossRefGoogle Scholar
  31. Patterson A (1939) The Scherrer formula for X-ray particle size determination. Phys Rev 56:978–982CrossRefGoogle Scholar
  32. Piotrowska-Niczyporuk A, Bajguz A, Zambrzycka E, Godlewska-Żyłkiewicz B (2012) Phytohormones as regulators of heavy metal biosorption and toxicity in green alga Chlorella vulgaris (Chlorophyceae). Plant Physiol Biochem 52:52–65CrossRefGoogle Scholar
  33. Reddy MK, Alexander-Lindo RL, Nair MG (2005) Relative inhibition of lipid peroxidation, cyclooxygenase enzymes, and human tumor cell proliferation by natural food colors. J Agric Food Chem 53:9268–9273CrossRefGoogle Scholar
  34. Song G, Gao Y, Wu H, Hou W, Zhang C, Ma H (2012) Physiological effect of anatase TiO2 nanoparticles on Lemna minor. Environ Toxicol Chem 31:2147–2152CrossRefGoogle Scholar
  35. Song G, Hou W, Gao Y, Wang Y, Lin L, Zhang Z, Niu Q, Ma R, Mu L, Wang H (2016) Effects of CuO nanoparticles on Lemna minor. Bot Stud 57:3–8CrossRefGoogle Scholar
  36. Tarrahi R, Khataee A, Movafeghi A, Rezanejad F, Gohari G (2017) Toxicological implications of selenium nanoparticles with different coatings along with Se4+ on Lemna minor. Chemosphere 181:655–665CrossRefGoogle Scholar
  37. Tian L, Ding J, Zhang W, Yang H, Fu W, Zhou X, Zhao W, Zhang L, Fan X (2011) Synthesis and photoelectric characterization of semiconductor CdSe microrod array by a simple electrochemical synthesis method. Appl Surf Sci 257:10535–10538CrossRefGoogle Scholar
  38. Verma S, Dubey R (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655CrossRefGoogle Scholar
  39. Wang Y, Zhu X, Lao Y, Lv X, Tao Y, Huang B, Wang J, Zhou J, Cai Z (2016) TiO2 nanoparticles in the marine environment: physical effects responsible for the toxicity on algae Phaeodactylum tricornutum. Sci Total Environ 565:818–826CrossRefGoogle Scholar
  40. Wang F, Guan W, Xu L, Ding Z, Ma H, Ma A, Terry N (2019) Effects of nanoparticles on algae: Adsorption, distribution, ecotoxicity and fate. Appl Sci 9:1534–1548CrossRefGoogle Scholar
  41. Xaaldi Kalhor A, Movafeghi A, Mohammadi-Nassab AD, Abedi E, Bahrami A (2017) Potential of the green alga Chlorella vulgaris for biodegradation of crude oil hydrocarbons. Mar pollut bull 123:286–290CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant Biology, Faculty of Natural SciencesUniversity of TabrizTabrizIran
  2. 2.Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of ChemistryUniversity of TabrizTabrizIran
  3. 3.Department of Environmental EngineeringGebze Technical UniversityGebzeTurkey
  4. 4.Drug Applied Research CenterTabriz University of Medical SciencesTabrizIran

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