Overexpression of AtAAP1 increased the uptake of an alanine-chlorantraniliprole conjugate in Arabidopsis thaliana

  • Zhanfu Ren
  • Zhiting Chen
  • Xiao Luo
  • Jiebing Su
  • Guangkai Yao
  • Hanhong XuEmail author
  • Fei LinEmail author
Research Article


Transporters play an important role in the uptake and redistribution of agrochemicals to the site of insect feeding. The product of the Arabidopsis thaliana gene AtAAP1 substantially contributes to inorganic nitrogen acquisition under ecologically relevant amino acid concentrations. Here, the transporter ability of AtAAP1 to a chlorantraniliprole-alanine conjugate (CAP-Ala-1) was tested both in planta and in vitro. Thirty-day-old and 15-day-old plants overexpressing AtAAP1 increased the uptake of CAP-Ala-1 into the roots, whereas AtAAP1 deficiency did not completely block the uptake of CAP-Ala-1. An uptake experiment carried out in Xenopus laevis oocytes expressing AtAAP1 showed that CAP-Ala-1 interacted with AtAAP1. Although little native AtAAP1 transcription was present in the leaves, constitutive expression of AtAAP1 in plants significantly increased the ability of the leaf mesophyll protoplasts to take up CAP-Ala-1. The observations supported the possibility of exploiting AtAAP1 as a component of a novel delivery and redistribution system for amino acid-based pesticide conjugates.


Alanine-chlorantraniliprole Amino acid transporter Guided pesticide Uptake Arabidopsis thaliana 


Funding information

This research was supported by the National Key R&D Program of China (2017YFD0200307 and 2017YFD0201100), the Project of Science and Technology in Guangdong Province (Grant No. 2018A030313188), and the Scientific Project in Guangzhou City (Grant No. 201707020013 and 201704030027).

Supplementary material

11356_2019_6671_MOESM1_ESM.pdf (127 kb)
ESM 1 (PDF 126 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesSouth China Agricultural UniversityGuangzhouChina
  2. 2.Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of EducationSouth China Agricultural UniversityGuangzhouChina
  3. 3.Guangdong Eco-engineering PolytechnicGuangzhouChina

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