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Overexpression of AtAAP1 increased the uptake of an alanine-chlorantraniliprole conjugate in Arabidopsis thaliana

  • Zhanfu Ren
  • Zhiting Chen
  • Xiao Luo
  • Jiebing Su
  • Guangkai Yao
  • Hanhong XuEmail author
  • Fei LinEmail author
Research Article

Abstract

Transporters play an important role in the uptake and redistribution of agrochemicals to the site of insect feeding. The product of the Arabidopsis thaliana gene AtAAP1 substantially contributes to inorganic nitrogen acquisition under ecologically relevant amino acid concentrations. Here, the transporter ability of AtAAP1 to a chlorantraniliprole-alanine conjugate (CAP-Ala-1) was tested both in planta and in vitro. Thirty-day-old and 15-day-old plants overexpressing AtAAP1 increased the uptake of CAP-Ala-1 into the roots, whereas AtAAP1 deficiency did not completely block the uptake of CAP-Ala-1. An uptake experiment carried out in Xenopus laevis oocytes expressing AtAAP1 showed that CAP-Ala-1 interacted with AtAAP1. Although little native AtAAP1 transcription was present in the leaves, constitutive expression of AtAAP1 in plants significantly increased the ability of the leaf mesophyll protoplasts to take up CAP-Ala-1. The observations supported the possibility of exploiting AtAAP1 as a component of a novel delivery and redistribution system for amino acid-based pesticide conjugates.

Keywords

Alanine-chlorantraniliprole Amino acid transporter Guided pesticide Uptake Arabidopsis thaliana 

Notes

Funding information

This research was supported by the National Key R&D Program of China (2017YFD0200307 and 2017YFD0201100), the Project of Science and Technology in Guangdong Province (Grant No. 2018A030313188), and the Scientific Project in Guangzhou City (Grant No. 201707020013 and 201704030027).

Supplementary material

11356_2019_6671_MOESM1_ESM.pdf (127 kb)
ESM 1 (PDF 126 kb)

References

  1. An YQ, McDowell JM, Huang S, McKinney EC, Chambliss S, Meagher RB (1996) Strong, constitutive expression of the Arabidopsis ACT2/ACT8 ctin subclass in vegetative tissues. Plant J 10:107-121.  https://doi.org/10.1046/j.1365-313x.1996.10010107.x CrossRefGoogle Scholar
  2. Chen LS, Bush DR (1997) LHT1, A lysine- and histidine-specific amino acid transporter in Arabidopsis. Plant Physiol 115:1127–1134CrossRefGoogle Scholar
  3. Chen Y et al (2018) AtLHT1 transporter can facilitate the uptake and translocation of a glycinergic-chlorantraniliprole conjugate in Arabidopsis thaliana. J Agric Food Chem 66:12527–12535.  https://doi.org/10.1021/acs.jafc.8b03591 CrossRefGoogle Scholar
  4. Damak M, Mahmoudi SR, Hyder MN, Varanasi KK (2016) Enhancing droplet deposition through in-situ precipitation. Nat Commun 7:12560.  https://doi.org/10.1038/ncomms12560 CrossRefGoogle Scholar
  5. Delétage-Grandon C, Chollet JF, Faucher M, Rocher F, Komor E, Bonnemain JL (2001) Carrier-mediated uptake and phloem systemyof a 350-Dalton chlorinated xenobiotic with an alpha-amino acid function. Plant Physiol 125:1620–1632CrossRefGoogle Scholar
  6. Denis M, Delrot S (1993) Carrier-mediated uptake of glyphosate in broad bean (Vicia faba) via a phosphate transporter. Physiol Plant 87:569–575CrossRefGoogle Scholar
  7. Hirner A, Ladwig F, Stransky H, Okumoto S, Keinath M, Harms A, Frommer WB, Koch W (2006) Arabidopsis LHT1 is a high-affinity transporter for cellular amino acid uptake in both root epidermis and leaf mesophyll. Plant Cell 18:1931–1946.  https://doi.org/10.1105/tpc.106.041012 CrossRefGoogle Scholar
  8. Hu AL, Yang W, Xu HH (2010) Novel fluorescent conjugate containing glucose and NBD and its carrier-mediated uptake by tobacco cells. J Photochem Photobiol B 101:215–223.  https://doi.org/10.1016/j.jphotobiol.2010.07.006 CrossRefGoogle Scholar
  9. Jiang X, Xie Y, Ren Z, Ganeteg U, Lin F, Zhao C, Xu HH (2018) Design of a new glutamine-gipronil conjugate with alpha-amino acid function and its uptake by A. thaliana lysine histidine transporter 1 ( AtLHT1). J Agric Food Chem 66:7597–7605.  https://doi.org/10.1021/acs.jafc.8b02287 CrossRefGoogle Scholar
  10. Lee YH, Foster J, Chen J, Voll LM, Weber AP, Tegeder M (2007) AAP1 transports uncharged amino acids into roots of Arabidopsis. Plant J 50:305–319.  https://doi.org/10.1111/j.1365-313X.2007.03045.x CrossRefGoogle Scholar
  11. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods 25:402–408.  https://doi.org/10.1006/meth.2001.1262 CrossRefGoogle Scholar
  12. Mao GL, Yan Y, Chen Y, Wang BF, Xu FF, Zhang ZX, Lin F, Xu HH (2017) Family of Ricinus communis monosaccharide transporters and RcSTP1 in promoting the uptake of a glucose-fipronil conjugate. J Agric Food Chem 65:6169–6178.  https://doi.org/10.1021/acs.jafc.7b02044 CrossRefGoogle Scholar
  13. Ortiz-Lopez A, Chang H-C, Bush DR (2000) Amino acid transporters in plants. Biochimica et Biophysica Acta 1465:275–280CrossRefGoogle Scholar
  14. Perchlik M, Foster J, Tegeder M (2014) Different and overlapping functions of Arabidopsis LHT6 and AAP1 transporters in root amino acid uptake. J Exp Bot65:5193–5204CrossRefGoogle Scholar
  15. Schröder P, Scheer CE, Diekmann F, Stampfl A (2007) How plants cope with foreign compounds. Environ Sci Pollut Res Int 14:114–122CrossRefGoogle Scholar
  16. Sheng QQ, Liu X, Xie Y, Lin F, Zhang ZX, Zhao C, Xu HH (2018) Synthesis of novel amino acid-fipronil conjugates and study on their phloem loading mechanism. Molecules 23.  https://doi.org/10.3390/molecules23040778 CrossRefGoogle Scholar
  17. Svennerstam H, Ganeteg U, Nasholm T (2008) Root uptake of cationic amino acids by Arabidopsis depends on functional expression of amino acid permease 5. New Phytol 180:620–630.  https://doi.org/10.1111/j.1469-8137.2008.02589.x CrossRefGoogle Scholar
  18. Svennerstam H, Jamtgard S, Ahmad I, Huss-Danell K, Nasholm T, Ganeteg U (2011) Transporters in Arabidopsis roots mediating uptake of amino acids at naturally occurring concentrations. New Phytol 191:459–467.  https://doi.org/10.1111/j.1469-8137.2011.03699.x CrossRefGoogle Scholar
  19. Tegeder M (2012) Transporters for amino acids in plant cells: some functions and many unknowns. Curr Opin Plant Biol 15:315–321CrossRefGoogle Scholar
  20. Vryzas Z (2016) The Plant as metaorganism and research on next-generation systemic pesticides - prospects and challenges. Front Microbiol 7.  https://doi.org/10.3389/fmicb.2016.01968
  21. Wang CJ, Liu ZQ (2007) Foliar uptake of pesticides—present status and future challenge. Pesticide Biochem Physiol 87:1–8.  https://doi.org/10.1016/j.pestbp.2006.04.004 CrossRefGoogle Scholar
  22. Wang T, Chen Y, Zhang M, Chen J, Liu J, Han H, Hua X (2017) Arabidopsis AMINO ACID PERMEASE 1 contributes to salt stress-induced proline uptake from exogenous sources. Front Plant Sci 8:2182.  https://doi.org/10.3389/fpls.2017.02182 CrossRefGoogle Scholar
  23. Wu HX, Yang W, Zhang ZX, Huang T, Yao GK, Xu HH (2012) Uptake and phloem transport of glucose-fipronil conjugate in Ricinus communis involve a carrier-mediated mechanism. J Agric Food Chem 60:6088–6094.  https://doi.org/10.1021/jf300546t CrossRefGoogle Scholar
  24. Wu H, Xu HH, Marivingt-Mounir C, Bonnemain JL, Chollet JF (2018) Vectorizing agrochemicals: enhancing bioavailability via carrier-mediated transport. Pest Manag Sci.  https://doi.org/10.1002/ps.5298 CrossRefGoogle Scholar
  25. Xie Y, Zhao JL, Wang CW, Yu AX, Liu N, Chen L, Lin F, Xu HH (2016) Glycinergic-fipronil uptake is mediated by an amino acid carrier system and induces the expression of amino acid transporter genes in Ricinus communis seedlings. J Agric Food Chem 64:3810–3818.  https://doi.org/10.1021/acs.jafc.5b06042 CrossRefGoogle Scholar
  26. Yao G, Wen Y, Zhao C, Xu HH (2017) Novel amino acid ester-chlorantraniliprole conjugates: design, synthesis, phloem accumulation and bioactivity. Pest Manag Sci 73:2131–2137.  https://doi.org/10.1002/ps.4592 CrossRefGoogle Scholar
  27. Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1:641–646.  https://doi.org/10.1038/nprot.2006.97 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesSouth China Agricultural UniversityGuangzhouChina
  2. 2.Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of EducationSouth China Agricultural UniversityGuangzhouChina
  3. 3.Guangdong Eco-engineering PolytechnicGuangzhouChina

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