Oxidative Stress and DNA methylation in male rat pups provoked by the transplacental and translactational exposure to bisphenol A
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The epigenetic changes induced by environmental contaminants play important roles in the inheritance of male reproductive dysfunction. The present study investigated DNA methylation changes and some oxidative stress biomarkers induced by bisphenol A (BPA) in male offspring. A total number of 48 female albino rats were administered orally with 50 μg/kg of BPA/day during gestation and/or lactation periods. At postnatal day 60, the samples were collected from the male pups to assess the serum testosterone, malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione S-transferase, and glutathione peroxidase (GSH-Px) activities in testicular tissue. DNA methylation in both DNA (cytosine-5)-methyltransferase 3A and estrogen receptor alpha genes was detected by methylation-specific PCR. BPA exposure resulted in significant decrease in the anogenital distance, testis and epididymis weights, serum testosterone level, SOD, GST, and GSH-Px levels with significant increase in weaning body weight and the MDA level. Additionally, BPA caused marked hypermethylation within Dnmt3A and ER- ∝ genes promoter regions in the testis of rat male pups.
KeywordsBisphenol A Epigenetics DNA hypermethylation Dnmt3A gene ER- ∝ gene
Samy Abd El Aziz contributed to design the experiment and interpretation of the results. Eman Gouda contributed to design, acquisition, and the analysis of data. Hanan El Henafy contributed to the analysis of data and drafted the manuscript. Marwa Ibrahim contributed to the analysis of data and drafted the manuscript. All authors approved the manuscript and ensured originality and accuracy.
Compliance with ethical standards
Animals were kept and treated according to the guidelines of the ethics committee of Cairo University (approval number CU II S 12 16).
Conflict of interest
The authors declare that they have no conflict of interest.
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