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Screening methane-oxidizing bacteria from municipal solid waste landfills and simulating their effects on methane and ammonia reduction

  • Jingran Pan
  • Xiaolin Wang
  • Aixin Cao
  • Guozhu ZhaoEmail author
  • Chuanbin ZhouEmail author
Research Article
  • 28 Downloads

Abstract

Municipal solid waste landfills are not only a crucial source of global greenhouse gas emissions; they also produce large amounts of ammonia (NH3), hydrogen sulfide, and other odorous gases that negatively affect the regional environment. Several types of methane-oxidizing bacteria (MOB) were proved to be effective in mitigating methane emission from landfills. Nevertheless, more MOB species and their technical parameters for best mitigating methane still need to be explored. In landfills, methane is simultaneously generated with ammonia, which may impede the CH4 bio-oxidizing process of MOB. However, very limited studies examined the enhancement of methane reduction by introducing ammonia-oxidizing bacteria (AOB) in landfills. In this study, two enriched MOB cultures were gained from a typical municipal solid waste landfill, and then were cultured with three strains of ammonia-oxidizing bacteria (AOB). The MOB enrichment culture used in this work includes Methylocaldum, Methylocystaceae, and Methyloversatilis, with a methane oxidation capacity of 43.6–65.0%, and the AOB includes Candida ethanolica, Bacillus cereus, and Alcaligenes faecalis. The effects on the emission reduction of both NH3 and CH4 were measured using self-made landfill-simulating equipment, as MOB, AOB, and a MOB–AOB mixture were added to the soil cover of the simulation equipment. The concentrations of CH4 and NH3 in the MOB–AOB mixture group decreased sharply, and the CH4 and NH3 concentration was 76.4% and 83.7% of the control group level. We also found that addition of AOB can help MOB oxidize CH4 and improve the emission reduction effect.

Keywords

Ammonia (NH3Methane (CH4Landfill Methane-oxidizing bacteria Reduction Ammonia-oxidizing bacteria (AOB) 

Notes

Acknowledgments

We were grateful to the reviewers and English native editors for helping us improve the paper. We also want to extend our sincere gratitude to the anonymous reviewers and the language editors.

Funding information

This work was financially supported by the Fundamental Research Funds for the Central Universities (2017JC01), the National Natural Science Foundation of China (No. 31570019, 41871206), National Key R&D Program of China (2016YFC0502800), and Youth Innovation Promotion Association CAS (2017061).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingChina
  2. 2.State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-environmental SciencesChinese Academy of SciencesBeijingChina

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