Effects of mutual grafting on cadmium accumulation characteristics of first post-generations of Bidens pilosa L. and Galinsoga parviflora Cav.

  • Hongyan Li
  • Jin Wang
  • Lijin LinEmail author
  • Ming’an Liao
  • Xiulan Lv
  • Yi Tang
  • Xun Wang
  • Hui Xia
  • Dong Liang
  • Wei Ren
  • Wei Jiang
Research Article


We studied the effects of mutual grafting on cadmium (Cd) accumulation characteristics on the first post-generations of the Cd-hyperaccumulator plants Bidens pilosa L. and Galinsoga parviflora Cav. The seeds from scions and rootstocks of B. pilosa and G. parviflora were collected and planted in Cd-contaminated soil in pot and field experiments. In the pot experiment, rootstock treatment increased the shoot biomass of B. pilosa post-grafting generations, compared with ungrafted B. pilosa, but decreased the Cd content in shoots and Cd extraction by shoots of post-grafting generations; scion treatment decreased or had no significant effect. Mutual grafting resulted in no significant differences to the photosynthetic pigment contents in B. pilosa post-grafting generations. Compared with ungrafted G. parviflora, scion treatment increased the shoot biomass, photosynthetic pigment content, and Cd extraction by shoots of G. parviflora post-grafting generations, but rootstock treatment did not lead to significant differences. Mutual grafting resulted in no significant differences to the Cd contents in shoots of G. parviflora post-grafting generations. In the field experiment, only rootstock treatment increased the shoot biomass of B. pilosa post-grafting generations, and only scion treatment increased the shoot biomass and the Cd extraction by shoots of G. parviflora post-grafting generations. Therefore, mutual grafting of scions may enhance the phytoremediation ability of G. parviflora first post-grafting generations.


Mutual grafting Cadmium Post-grafting generation Hyperaccumulator Bidens pilosa Galinsoga parviflora 



  1. Arao T, Takeda H, Nishihara E (2008) Reduction of cadmium translocation from roots to shoots in eggplant (Solanum melongena) by grafting onto Solanum torvum rootstock. J Plant Nutr Soil Sci 54:555–559CrossRefGoogle Scholar
  2. Bao SD (2000) Soil agricultural chemistry analysis. China Agricultural Publishing House, BeijingGoogle Scholar
  3. Bautista AS, Calatayud A, Sergio GN, Pascual B, Maroto JV, López-Galarza S (2011) Effects of simple and double grafting melon plants on mineral absorption, photosynthesis, biomass and yield. Sci Hortic 130:575–580CrossRefGoogle Scholar
  4. Boyko A, Kovalchuk I (2008) Epigenetic control of plant stress response. Environ Mol Mutagen 49:61–72CrossRefGoogle Scholar
  5. Cao LW (2008) Studies on the molecular mechanism of phenotypic variations in the progenies of chimeras produced by in vitro grafting between Brassica iuncea and B. oleracea (Master Thesis). Zhejiang UniversityGoogle Scholar
  6. Carmina G, Jaime P, María DR, John RS, Fernando N (2011) Eggplant relatives as sources of variation for developing new rootstocks: effects of grafting on eggplant yield and fruit apparent quality and composition. Sci Hortic 128:14–22CrossRefGoogle Scholar
  7. Chen GL, Nie CL (2004) Practical techniques of vegetable grafting cultivation. Jindun Publishing House, BeijingGoogle Scholar
  8. Chinnusamy V, Zhu JK (2009) Epigenetic regulation of stress responses in plants. Curr Opin Plant Biol 12:133–139CrossRefGoogle Scholar
  9. Edelstein M, Ben-Hur M (2007) Preventing contamination of supply chains by using grafted plants under irrigation with marginal water. In: Wilson J. (Ed.) Proceedings of the International Symposiumon Water Resources Management, Honolulu, HawaiiGoogle Scholar
  10. Fu L, Bai XM, Yang XH, Wu GX, Ai XZ (2013) Effects of grafted on photosynthetic characteristics, yield and quality of pepper. Acta Horticult Sin 40:449–457Google Scholar
  11. Gisbert C, Sánchez-Torres P, Raigón M, Nuez F (2010) Phytophthora capsici resistance evaluation in pepper hybrids: agronomic performance and fruit quality of pepper grafted plants. J Food Agric Environ 8:116–121Google Scholar
  12. Guan XS (2016) Grafting induced biological variation in Cucumis sativus L. and its distant hybridization (Master Thesis), Fujian Agricultural and Forestry UniversityGoogle Scholar
  13. Guo DY, Huang JQ, Fang YM (2004) Summary of research on grafting mechanisms of plants. Acta Agric Univ Jiangxiensis 26:144–148Google Scholar
  14. Hao ZB, Cang J, Xu Z (2004) Plant physiological experiments. Harbin University of Technology Press, HarbinGoogle Scholar
  15. Hendrik P, Karl JN, Peter BR, Jacek O, Pieter P, Liesje M (2012) Biomass allocation to leaves, stems and roots: meta-analyses of interspecific variation and environmental control. New Phytol 193:30–50CrossRefGoogle Scholar
  16. Huang X, Hong J, Zhang LH, Ge MH, Ye LX, Wang L, Chen T, Chen G (2013) Multiple regression and path analysis between chlorophyll content, fluorescence kinetic parameters and watermelon yield. Hubei Agric Sci 52:4953–4955Google Scholar
  17. Jiang WB, Gao GL, Yu KJ, Wang LJ, Ma K (2002) A review of studies on the effects of water stress on photosynthesis and assimilation metabolism of fruit crops. J Fruit Sci 19:416–420Google Scholar
  18. Kirk JTO (1994) Light and photosynthesis in aquatic ecosystems (2nd edition). Cambridge University Press, New YorkGoogle Scholar
  19. Lin LJ, Jin Q, Liu Y, Ning B, Liao MA, Luo L (2014) Screening of a new cadmium hyperaccumulator, Galinsoga parviflora, from winter farmland weeds using the artificially high soil cadmium concentration method. Environ Toxicol Chem 33:2422–2428CrossRefGoogle Scholar
  20. Lin LJ, Luo L, Zhang X, Yang DY, Liao MA, Tang FY (2015) Effects of rape rootstock on cadmium accumulation characteristics of Capsella bursa-pastoris post-grafting generation. Acta Agriculturae Boreall Sin 30:207–212Google Scholar
  21. Lin LJ, Yang DY, Wang X, Liao MA, Wang ZH, Lv XL, Tang FY, Liang D, Xia H, Lai YS, Tang Y (2016) Effects of grafting on the cadmium accumulation characteristics of the potential Cd-hyperaccumulator Solanum photeinocarpum. Environ Monit Assess 188:82–94CrossRefGoogle Scholar
  22. Liu HY, Zhu ZJ, Shi QH (2007) Effects of low temperature stress on characteristics of photosynthesis in leaves of own-rooted and grafted watermelon seedling. J Shihezi Univ (Nat Sci) 25:163–167Google Scholar
  23. Liu YS, Wang QL, Li BY (2010) New insights into plant graft hybridization. Heredity 104:1–2CrossRefGoogle Scholar
  24. Ma TS, Hao GL (2008) Grafting variation of plant. Biol Teach 33:69–70Google Scholar
  25. Nie FH (2004) New comprehensions of hyperaccumulator. Ecol Environ 14:136–138Google Scholar
  26. Pan XW, Sun XH, Zhang FY, Zhao C, Zhang XS, Du WG (2012) Optimization of distant grafting mutagenesis technology in soybean. Soybean Sci 31:237–241Google Scholar
  27. Rastmanesh F, Moore F, Keshavarzi B (2010) Speciation and phytoavailability of heavy metals in contaminated soils in Sarcheshmeh area, Kerman Province, Iran. B Environ Contam Tox 85:515–519CrossRefGoogle Scholar
  28. Sun YB, Zhou QX, Wang L, Liu WT (2009) Cadmium tolerance and accumulation characteristics of Bidens pilosa L. as a potential Cd-hyperaccumulator. J Hazard Mater 161:808–814CrossRefGoogle Scholar
  29. Taller J, Yagishita N, Hirata Y (1999) Graft-induced variants as a source of novel characteristics in the breeding of pepper. Euphytlca 108:73–78CrossRefGoogle Scholar
  30. Tang QX, Miu S (2013) Progress of remediation on soil polluted by cadmium. Environ Eng Sci 31:747–750Google Scholar
  31. Wang Y, Xie H, Chen LP (2011) Progress in research on plant graft-induced genetic variation. Hereditas 33:585–590CrossRefGoogle Scholar
  32. Wang J, Lin LJ, Liu L, Liang D, Xia H, Lv XL, Liao MA, Wang ZH, Lai YS, Tang Y, Wang X, Ren W (2016) Interspecies rootstocks affect cadmium accumulation in postgrafting generation plants of potential cadmium-hyperaccumulator Solanum photeinocarpum. Environ Toxicol Chem 35:2845–2850CrossRefGoogle Scholar
  33. Wei SH, Zhou QX, Wang X, Zhang KS, Guo GL, Ma LNQY (2005) A newly-discovered Cd-hyperaccumulator Solanum nigrum L. Chin Sci Bull 50:33–38CrossRefGoogle Scholar
  34. Weng ZX, Li BD, Feng DX (1993) Study on disease control and yield increase of grafted cucumber. China Veg 12:11–15Google Scholar
  35. Wu R, Wang XR, Lin Y, Ma YQ, Liu G, Yu XM, Zhong SL, Liu B (2013) Inter-species grafting caused extensive and heritable alterations of DNA methylation in Solanaceae plants. PLoS One 8:e61995CrossRefGoogle Scholar
  36. Yagishta N, Hirata Y (1984) Genetic analysis of the new cultivar obtained by grafting in Capsicum annuum L. Jap J Breed 34:58–59Google Scholar
  37. Yao H, Zhang F, Qing M, Chen M, Lu Z, Zhang Q, Lin L, Liao M, Chen S, Huang Z, Chen C, Ren W (2019) Effects of mutual grafting on the cadmium accumulation characteristics of two ecotypes of Solanum photeinocarpum. Int J Phytoremediat 21:503–508CrossRefGoogle Scholar
  38. Zhang X, Qin ZG, Fei LS, Lou XY (2008) Research progress in the application of grafting technique in plant breeding. J Anhui Agric Sci 36(2333–2334):2379Google Scholar
  39. Zhang X, Xia H, Li Z, Zhuang P, Gao B (2011) Identification of a new potential Cd-hyperaccumulator Solanum photeinocarpum by soil seed bank-metal concentration gradient method. J Hazard Mater 189:414–419CrossRefGoogle Scholar
  40. Zhang X, Zhang F, Wang J, Lin L, Liao M, Tang Y, Sun G, Wang X, Lv X, Deng Q, Chen C, Ren W (2019) Cutting after grafting affects the growth and cadmium accumulation of Nasturtium officinale. Environ Sci Pollut Res 26:15436–15442CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Pomology and OlericultureSichuan Agricultural UniversityChengduChina
  2. 2.College of HorticultureSichuan Agricultural UniversityChengduChina
  3. 3.Maize Research InstituteNeijiang Academy of Agricultural SciencesNeijiangChina
  4. 4.College of Chemistry and Life ScienceChengdu Normal UniversityChengduChina

Personalised recommendations