Fungal proliferation and hydrocarbon removal during biostimulation of oily sludge with high total petroleum hydrocarbon

  • Hanghai Zhou
  • Xiaomin Huang
  • Kuiyong Bu
  • Fang Wen
  • Dongdong ZhangEmail author
  • Chunfang ZhangEmail author
Research Article


A laboratory-scale study was conducted to investigate the effect of bioaugmentation (BA) and biostimulation (BS) on the remediation of oily sludge with high total petroleum hydrocarbon (TPH) content (269,000 mg/kg d.w. sludge). TPH concentration significantly decreased by 30.4% (P < 0.05) in the BS treatment after 13-week incubation, and 17.0 and 9.1% of TPH was removed in the BA and control treatments (amended with sterile water only), respectively. Aliphatic and other fractions (i.e., saturated n-alkanes and cyclic saturated alkanes) were reduced in the BS treatment, whereas no decrease in aromatic hydrocarbons occurred in any treatment. Gas chromatography–mass spectrometry analysis of aliphatic fractions showed that low-chain-length alkanes (C8–C20) were the most biodegradable fractions. The BS treatment supported fungal proliferation, with Sordariomycetes and Eurotiomycetes as the dominant classes. BS increased fungal diversity and decreased fungal abundance, and changed bacterial community structure. The findings show the potential of using BS to treat oily sludge with high TPH content.

Graphical abstract


Oily sludge Biostimulation Bioaugmentation Total petroleum hydrocarbon Fungal proliferation 


Funding information

This work was supported by XinJiang Keli New Technology Development Co., Ltd. (K17-529102-004), Karamay Major Science and Technology Project (2018ZD003B), and National Key Research and Development Program of China (2016YFE0123800).

Compliance with ethical standards

Conflicts of interest

The authors declare that there is no conflict of interest.

Supplementary material

11356_2019_6432_MOESM1_ESM.docx (41 kb)
ESM 1 (DOCX 41 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Marine Biology, Ocean CollegeZhejiang UniversityZhoushanChina
  2. 2.Xinjiang Keli New Technology Development Co., Ltd.KaramayChina
  3. 3.Xinjiang Academy of Environmental Protection ScienceUrumqiChina

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