Lead accumulation and soil microbial activity in the rhizosphere of the mining and non-mining ecotypes of Athyrium wardii (Hook.) Makino in adaptation to lead-contaminated soils
- 20 Downloads
Better understanding of microbial activity in the rhizosphere soils associated with lead (Pb) uptake by plants may help with the phytoremediation of Pb-contaminated soils. In this work, the effects of Pb exposure (0, 200, 400, 600, 800 mg kg−1) on Pb accumulation and soil microbial activity in the rhizosphere of the mining ecotype (ME) and corresponding non-mining ecotype (NME) of Athyrium wardii (Hook.) Makino were investigated through a pot experiment. Although the plant growth of the two ecotypes was inhibited under Pb stress, the ME showed a less biomass decrease (12.6–44.0%) for aboveground than the NME, showing a greater tolerance to Pb stress. Pb concentrations as well as Pb accumulation in the two ecotypes showed an increasing trend with increasing soil Pb concentrations. The ME presented greater Pb accumulation ability than the NME, especially in underground parts. Pb availability in the rhizosphere soils of the two ecotypes after harvest decreased compared with those before transplantation. Available Pb in the rhizosphere of the ME was 1.4–4.8 times higher than that of the NME under exposure to 200–800 mg kg−1 Pb. The ME shows a greater ability to mobilize Pb in the rhizosphere soils. Pb exposure resulted in an inhibition of microbial activity in the rhizosphere of the two ecotypes. The ME demonstrated greater soil respiration and microbial biomass carbon (MBC) in the rhizosphere than the NME when treated with 200–800 mg kg−1 Pb. The ME showed a less decrease for MBC and a less increase for metabolic quotient in the rhizosphere soils than the NME when exposed to Pb generally. Microorganisms in the rhizosphere soils of the ME seem to be much more adapted to Pb stress, thus showing a great benefit for Pb accumulation and the phytostabilization of Pb-contaminated soils by the ME.
KeywordsPb Accumulation Microbial activity Rhizosphere Phytostabilization Athyrium wardii (Hook.) Makino
This study was financially supported by the National Key Research and Development Program (2018YFD0800600) and Sichuan Key Research Programs (2017SZ0188, 2017SZ0198, and 2018SZ0326).
- Bolan NS, Park JH, Robinson B, Naidu R, Huh KY (2011) Phytostabilization: a green approach to contaminant containment. In: Advances in agronomy. Academic, Cambridge, pp 145–204Google Scholar
- Doronila AI, Maddox LE, Reichman SM, King DJ, Kolev SD, Woodrow IE (2014) Vegetation response of Australian native grass species red grass (Bothriochloa macra (Steudel) ST Blake) and spider grass (Enteropogon acicularis (Lindl.) Lazarides) in saline and arsenic contaminated gold mine tailings: a glasshouse study. Miner Eng 56:61–69CrossRefGoogle Scholar
- Lu RK (1999) Analysis of soil agrochemistry. Chinese Agricultural Science and Technology Press, Beijing, pp 1–246 (in Chinese)Google Scholar
- Shakoor MB, Ali S, Farid M, Farooq MA, Tauqeer HM, Iftikhar U, Hannan F, Bharwana SA (2013) Heavy metal pollution, a global problem and its remediation by chemically enhanced phytoremediation, a review. J Biol Environ Sci 3:12–20Google Scholar
- Wang YP, Li QB, Shi JY, Lin Q, Chen XC, Wu WX, Chen YX (2008) Assessment of microbial activity and bacterial community composition in the rhizosphere of a copper accumulator and a non-accumulator. Soil Biol Biochem 40:1167–1177Google Scholar
- Zhang SJ, Li TX, Zhang XZ, Yu HY, Zheng ZC, Wang YD, Hao XQ, Pu Y (2014) Changes in pH, dissolved organic matter and Cd species in the rhizosphere soils of Cd phytostabilizer Athyrium wardii (Hook.) Makino involved in Cd tolerance and accumulation. Environ Sci Pollut Res 21:4605–4613CrossRefGoogle Scholar