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Environmental Science and Pollution Research

, Volume 26, Issue 31, pp 32488–32504 | Cite as

Protective effect of Moringa oleifera leaves ethanolic extract against thioacetamide-induced hepatotoxicity in rats via modulation of cellular antioxidant, apoptotic and inflammatory markers

  • Ahmed Abdelmoniem Mousa
  • Hala Ali Ibrahim El-Gansh
  • Mabrouk Attia Abd EldaimEmail author
  • Mostafa Abd El-Gaber Mohamed
  • Azza Hassan Morsi
  • Hesham Saad El Sabagh
Research Article
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Abstract

The current study was conducted to evaluate the ameliorative and protective potentials of Moringea oleifera leaves ethanolic extract (MOLE) against thioacetamide (TAA) toxicity. A total of 58 male albino rats were randomly assigned into six experimental groups. G1, rats received distilled water. G2, rats were injected with a single dose of TAA (200 mg/kg BW) i.p. G3, rats were given MOLE (300 mg/kg BW) orally for 26 days. G4, rats were injected TAA as in G2 and treated with MOLE as G3. G5, rats were kept for 26 days without treatment then on day 27 injected with TAA as in G2. G6, rats were given MOLE for 26 days then on day 27 injected with TAA. Phytochemical analysis of MOLE indicated the presence of kaempferol, kaempferol malonylglucoside, kaempferol hexoside, kaempferol -3-O-glucoside, kaempferol-3-O-acetyl-glucoside, cyanidin -3-O-hexoside, ellagic acid, quercetin, quercetin-3-O-glucoside, and apigenin glucoside. Intoxication of rats with TAA significantly elevated activities of serum AST, ALT, and ALP; concentrations of malondialdehyde, nitric oxide, and hepatic tissue protein expression of caspase 3 and COX2 with alteration of the histological structures of hepatic tissues, while it decreased serum levels of total protein, albumin, and hepatic tissue contents of reduced glutathione. Also, TAA intoxication resulted in 62.5% mortality in rats of G5. Treatment of TAA intoxicated rats (G4) with MOLE ameliorated the toxic effects of TAA on hepatic tissue structure and function. It decreased serum activities of AST, ALT, and ALP; enhanced hepatic GSH concentration; reduced pathological alterations and lipid peroxidation; and downregulated caspase 3 and COX2 proteins expression in hepatic tissue. In addition, MOLE protected rats of G6 from TAA-induced hepatic tissues injury and dysfunction, and increased survival rate of rats. In conclusion, MOLE had both ameliorating and protecting potentials against TAA-induced rats liver damage through regulation of antioxidant, anti-apoptotic, and inflammatory biomarkers.

Graphical abstract

Keywords

Moringea oleifera leaves ethanolic extract Liver toxicity Caspase3 COX2 
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Notes

Acknowledgments

The phytochemical analysis in this manuscript was performed by the help of Professor Hesham El-Seedi, Department of Chemistry, Faculty of Science, Menoufia University, Egypt, and Pharmacognosy Group, Department of Medicinal Chemistry, Uppsala University, Sweden.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ahmed Abdelmoniem Mousa
    • 1
  • Hala Ali Ibrahim El-Gansh
    • 1
  • Mabrouk Attia Abd Eldaim
    • 2
    Email author
  • Mostafa Abd El-Gaber Mohamed
    • 3
  • Azza Hassan Morsi
    • 4
  • Hesham Saad El Sabagh
    • 5
  1. 1.Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt
  2. 2.Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary MedicineMenoufia UniversityShebin ElkomEgypt
  3. 3.Department of Pathology, Faculty of Veterinary MedicineMenoufia UniversityShebin ElkomEgypt
  4. 4.Department of Pathology, Faculty of Veterinary MedicineCairo UniversityCairoEgypt
  5. 5.Department of Toxicology and Forensic Medicine, Faculty of Veterinary MedicineUniversity of Sadat CitySadat CityEgypt

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