Effects of ozone on the growth and yield of rice (Oryza sativa L.) under different nitrogen fertilization regimes

  • Kenichi TatsumiEmail author
  • Tamami Abiko
  • Yoshiyuki Kinose
  • Shiro Inagaki
  • Takeshi Izuta
Research Article


To examine whether the sensitivity of growth and yield of rice (Oryza sativa L.) to ozone (O3) varies under different nitrogen (N) fertilization conditions, rice cultivar ‘Koshihikari’ was exposed to O3 under either standard N (SN) fertilization or no N (NN) fertilization. The rice plants were subjected to three gas treatments (charcoal-filtered air (CF) and O3 at 1.0 (1.0×O3) and 1.5 (1.5×O3) times the ambient concentration) in combination with two conditions of N fertilization. At five time points throughout the growth period, plant samples were collected to measure the leaf area and dry mass of each plant organ. At the final harvest, yield, yield components, and harvest index were measured. There was a significant interactive effect of O3 and N on leaf, stem, root, and whole-plant dry mass at the final harvest. The dry mass of each plant organ and the whole-plant dry mass of rice plants grown in 1.5×O3 were significantly lower than those in the plants grown in CF and 1.0×O3 under SN, whereas there were no significant differences in the dry mass among the three gas treatments under NN. Brown rice yield was significantly reduced by the exposure to O3 under SN, but not under NN. Relative yield loss rate based on the AOT40 (accumulated exposure over a threshold of 40 nmol mol−1) was pronounced under SN, whereas relative yield was almost unchanged at different AOT40 levels under NN. We concluded that the sensitivity of growth and yield of rice to O3 is dependent on N levels in the soil; the exposure to ambient levels of O3 has a negative effect on rice under SN, but not under NN.


Dry mass Growth Nitrogen Ozone Rice Yield 



We thank Yoshiki Kuwabara and Ryo Sekino from the Tokyo University of Agricultural and Technology, Japan, for their support with measurements.

Funding information

This work was supported by the Japan Science and Technology Agency (PRESTO Grant Number JPMJPR1603) and by the Japan Society for the Promotion of Science (KAKENHI Grant Number 16K18778).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Environmental and Agricultural Engineering, Institute of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan
  2. 2.Japan Science and Technology AgencyKawaguchiJapan
  3. 3.Department of Ecoregion Science, Faculty of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan
  4. 4.Department of Environmental Sciences, Faculty of Life and Environmental SciencesUniversity of YamanashiKofuJapan
  5. 5.Department of Environmental and Natural Resource Sciences, Faculty of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan
  6. 6.Division of Environmental Science on Biosphere, Institute of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan

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