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Environmental Science and Pollution Research

, Volume 26, Issue 31, pp 32255–32265 | Cite as

Control of a toxic cyanobacterial bloom species, Microcystis aeruginosa, using the peptide HPA3NT3-A2

  • Sang-Il Han
  • Sok Kim
  • Ki Young Choi
  • Changsu Lee
  • Yoonkyung ParkEmail author
  • Yoon-E ChoiEmail author
Research Article

Abstract

Microcystis aeruginosa, a species of freshwater cyanobacteria, is known to be one of the dominant species causing cyanobacterial harmful algal blooms (CyanoHABs). M. aeruginosa blooms have the potential to produce neurotoxins and peptide hepatotoxins, such as microcystins and lipopolysaccharides (LPSs). Currently, technologies for CyanoHAB control do not provide any ultimate solution because of the secondary pollution associated with the control measures. In this study, we attempted to use the peptide HPA3NT3-A2, which has been reported to be nontoxic and has antimicrobial properties, for the development of an eco-friendly control against CyanoHABs. HPA3NT3-A2 displayed significant algicidal effects against M. aeruginosa cells. HPA3NT3-A2 induced cell aggregation and flotation (thereby facilitating harvest), inhibited cell growth through sedimentation, and eventually destroyed the cells. HPA3NT3-A2 had no algicidal effect on other microalgal species such as Haematococcus pluvialis and Chlorella vulgaris. Additionally, HPA3NT3-A2 was not toxic to Daphnia magna. The algicidal mechanism of HPA3NT3-A2 was intracellular penetration. The results of this study suggest the novel possibility of controlling CyanoHABs using HPA3NT3-A2.

Keywords

Cyanobacterial blooms HABs Microcystis aeruginosa Eco-friendly mitigation Algicide Algicidal peptide HPA3NT3-A2 

Notes

Funding information

This work was supported by the Government of South Korea through the National Research Foundation of Korea (NRF-2016R1D1A1B03932773) and Korea Basic Science Institute under the R&D Program (Project No. C38703), supervised by the Ministry of Science, ICT and Future Planning. This research was also supported by the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) (No. 20170488).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

11356_2019_6306_MOESM1_ESM.docx (108 kb)
ESM 1 (DOCX 108 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Environmental Science & Ecological EngineeringKorea UniversitySeoulKorea
  2. 2.School of Chemical Engineering, College of EngineeringSungkyunkwan UniversitySuwonKorea
  3. 3.Microbiology and Functionality Research GroupWorld Institute of KimchiGwangjuKorea
  4. 4.Research Center for Proteinaceous Materials (RCPM)Chosun UniversityGwangjuKorea

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