Prevalence of antibiotic resistance genes in cell culture liquid waste and the virulence assess for isolated resistant strains
Cell culture liquid waste containing antibiotic resistance genes (ARGs) and microbial community were still not received enough recognition, which pose potential risks to human health. Sixty-eight resistance genes and intl1 were detected in eight samples by Quantitative real-time PCR, while intl1 was only detected in hospital group. Meanwhile, the bacterial community was complex and diverse in each sample by 16S rRNA gene high-throughput sequencing, in addition, Morganella and Enterococcus presented a significant difference between two groups. Whole genome shotgun sequencing revealed that Morganella morganii had more resistance genes and virulence factors in hospital group, and three extended-spectrum beta-lactamase (ESBL) genotypes were found to be blaDHA-5, blaOXA-1, and blaTEM-1. This study provided a preliminary report on ARGs and resistant strains, which reminded people attention to the health risks of potential pathogens in this waste.
KeywordsCell culture liquid waste Antibiotic resistance genes Morganella morganii ESBLs Virulence factor
We gratefully acknowledged the support of Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences and Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard.
This work was funded by the Graduate Innovation Program of Beijing Jiaotong University (2017YJS155) and the Open Project funded by the State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2017-17).
- Azevedo PA, Gonçalves ALS, Musskopf MI, Ramos CG, Dias CAG (2004) Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing. Braz J Infect Dis 8:372–377. https://doi.org/10.1590/S1413-86702004000500006 CrossRefGoogle Scholar
- Baudry PJ et al (2009) Mechanisms of resistance and mobility among multidrug-resistant CTX-M-producing Escherichia coli from Canadian intensive care units: the 1st report of QepA in North America. Diagn Microbiol Infect Dis 63:319–326. https://doi.org/10.1016/j.diagmicrobio.2008.12.001 CrossRefGoogle Scholar
- Farman M, Yasir M, Al-Hindi RR, Farraj SA, Jiman-Fatani AA, Alawi M, Azhar EI (2019) Genomic analysis of multidrug-resistant clinical Enterococcus faecalis isolates for antimicrobial resistance genes and virulence factors from the western region of Saudi Arabia. Antimicrob Resist Infect Control 8:55. https://doi.org/10.1186/s13756-019-0508-4 CrossRefGoogle Scholar
- Gopireddy VR (2011) Biochemical tests for the identification of bacteria. Int J Pharm Technol Prod Manuf 3:1536–1555Google Scholar
- Hövel H, Frieling KH (1987) The use of doxycycline, mezlocillin and clotrimazole in cell culture media as contamination prophylaxis. Dev Biol Stand 66:23Google Scholar
- Hsu JT, Chen CY, Young CW, Chao WL, Li MH, Liu YH, Lin CM, Ying C (2014) Prevalence of sulfonamide-resistant bacteria, resistance genes and integron-associated horizontal gene transfer in natural water bodies and soils adjacent to a swine feedlot in northern. Taiwan. J Hazard Mater 277:34–43. https://doi.org/10.1016/j.jhazmat.2014.02.016 CrossRefGoogle Scholar
- Hyun SW, Kim BR, Lin D, Hyun SA, Yoon SS, Seo JW (2018) The effects of gentamicin and penicillin/streptomycin on the electrophysiology of human induced pluripotent stem cell-derived cardiomyocytes in manual patch clamp and multi-electrode array system. J Pharmacol Toxicol Methods 91:1–6. https://doi.org/10.1016/j.vascn.2017.12.002 CrossRefGoogle Scholar
- Kristich CJ, Rice LB, Arias CA (2014) Enterococcal infection-treatment and antibiotic resistance. In: Gilmore MS, Clewell DB, Ike Y, Shankar N (eds) Enterococci: from commensals to leading causes of drug resistant infection. Massachusetts Eye and Ear Infirmary, BostonGoogle Scholar
- Liao K et al (2017) Molecular characteristics of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae causing intra-abdominal infections from 9 tertiary hospitals in China. Diagn Microbiol Infect Dis 87:45–48. https://doi.org/10.1016/j.diagmicrobio.2016.10.007 CrossRefGoogle Scholar
- Mahrouki S, Perilli M, Bourouis A, Chihi H, Ferjani M, Ben Moussa M, et al (2013) Prevalence of quinolone resistance determinant qnrA6 among broad- and extended-spectrum beta-lactam-resistant Proteus mirabilis and Morganella morganii clinical isolates with sul1-type class 1 integron association in a Tunisian Hospital. Scandinavian Journal of Infectious Diseases 45(8):600–5Google Scholar
- Olaitan AO, Diene SM, Gupta SK, Adler A, Assous MV, Rolain J (2014) Genome analysis of NDM-1 producing Morganella morganii clinical isolate. Expert Review of Anti-infective Therapy 12(10):1297–305Google Scholar
- Rodriguez-Mozaz S, Chamorro S, Marti E, Huerta B, Gros M, Sànchez-Melsió A, Borrego CM, Barceló D, Balcázar JL (2015) Occurrence of antibiotics and antibiotic resistance genes in hospital and urban wastewaters and their impact on the receiving river. Water Res 69:234–242. https://doi.org/10.1016/j.watres.2014.11.021 CrossRefGoogle Scholar
- Younessi N, Safari Sinegani AA, Khodakaramian G (2019) Detection of antibiotic resistance genes in culturable bacteria isolated from soils around mines in Hamedan, Iran. Int J Environ Sci Technol. https://doi.org/10.1007/s13762-018-02178-2
- Zhang Q-Q, Tian G-M, Jin R-C (2018) The occurrence, maintenance, and proliferation of antibiotic resistance genes (ARGs) in the environment: influencing factors, mechanisms, and elimination strategies. Appl Microbiol Biotechnol 102:8261–8274. https://doi.org/10.1007/s00253-018-9235-7 CrossRefGoogle Scholar