Prevalence of antibiotic resistance genes in cell culture liquid waste and the virulence assess for isolated resistant strains

  • Haichao Wang
  • Jin WangEmail author
  • Shuming Li
  • Jinzhao Li
  • Chuanyong Jing
Research Article


Cell culture liquid waste containing antibiotic resistance genes (ARGs) and microbial community were still not received enough recognition, which pose potential risks to human health. Sixty-eight resistance genes and intl1 were detected in eight samples by Quantitative real-time PCR, while intl1 was only detected in hospital group. Meanwhile, the bacterial community was complex and diverse in each sample by 16S rRNA gene high-throughput sequencing, in addition, Morganella and Enterococcus presented a significant difference between two groups. Whole genome shotgun sequencing revealed that Morganella morganii had more resistance genes and virulence factors in hospital group, and three extended-spectrum beta-lactamase (ESBL) genotypes were found to be blaDHA-5, blaOXA-1, and blaTEM-1. This study provided a preliminary report on ARGs and resistant strains, which reminded people attention to the health risks of potential pathogens in this waste.


Cell culture liquid waste Antibiotic resistance genes Morganella morganii ESBLs Virulence factor 



We gratefully acknowledged the support of Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences and Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality Safeguard.

Funding information

This work was funded by the Graduate Innovation Program of Beijing Jiaotong University (2017YJS155) and the Open Project funded by the State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2017-17).

Supplementary material

11356_2019_6299_MOESM1_ESM.docx (1 mb)
ESM 1 (DOCX 1066 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Civil Engineering, Beijing Key Laboratory of Aqueous Typical Pollutants Control and Water Quality SafeguardBeijing Jiaotong UniversityBeijingChina
  2. 2.China Shenhua Overseas Development & Investment Co., LimitedBeijingChina
  3. 3.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina

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