Environmental Science and Pollution Research

, Volume 26, Issue 30, pp 31234–31242 | Cite as

Effect of carbon source on lipid accumulation and biodiesel production of Yarrowia lipolytica

  • Baohua Chai
  • Yi WangEmail author
  • Wenhuai Wang
  • Pan Fan
Research Article


Yarrowia lipolytica (Y. lipolytica) is an oleaginous yeast that can utilize hydrophobic substrates as carbon source to produce single-cell lipids for biodiesel production. This study attempts to increase the lipid accumulation ability of Y. lipolytica by first gradually elevating pure oil substrate concentration during the cultivation and then adding short-chain carbon compounds, such as glucose and sodium acetate, to a culture substance according to the optimal oil concentration. Results showed that Y. lipolytica cultured under 40.0 g L−1 oil concentration showed higher lipids (2.97 g L−1) and lipid content (37.35%, DW) compared with that cultured under 20.0 g L−1, where the corresponding values were 1.91 g L−1 and 24.46%. By contrast, the lipid content of Y. lipolytica increased from 37.35 to 41.50% when the substrate was changed from 40.0 g L−1 pure oil to 5% sodium acetate combined with 95% oil under the same total carbon concentration. However, lipid accumulation did not increase even though 15% sodium acetate or 5% glucose, or 15% glucose was added to the combined substrate. Moreover, the lipid biomodification of Y. lipolytica was evident when it was cultured under the oil concentration of 20.0 g L−1. Therefore, the lipid accumulation of Y. lipolytica can be elevated through the gradient increase of oil concentration and by adding a suitable amount of easily degradable carbon source. Furthermore, the lipid biomodification of Y. lipolytica improves biodiesel quality.


Oleaginous yeast Biomodification Oily wastewater Lipids content Resource recovery 


Funding information

This work was supported by the National Natural Science Foundation of China (No. 21677115) and the Shaanxi Provincial Natural Science Foundation Research Key Project (No. 2016JZ019).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Environmental and Municipal EngineeringXi’an University of Architecture and TechnologyXi’anChina
  2. 2.Shaanxi Key Laboratory of Environmental EngineeringXi’an University of Architecture and TechnologyXi’anPeople’s Republic of China
  3. 3.Key Laboratory of Northwest Water Resource, Environment and Ecology, MOEXi’an University of Architecture and TechnologyXi’anPeople’s Republic of China

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