Environmental Science and Pollution Research

, Volume 26, Issue 30, pp 31215–31224 | Cite as

Hepatoprotective effects of the n-butanol extract from Perralderia coronopifolia Coss. against PCP-induced toxicity in Wistar albino rats

  • Khadidja Bekhouche
  • Tevfik OzenEmail author
  • Sara Boussaha
  • Ibrahim Demirtas
  • Mounir Kout
  • Kemal Yildirim
  • Djamila Zama
  • Fadila Benayache
  • Samir Benayache
Research Article


In the present study, in vivo antioxidant properties of the n-butanol extract obtained from aerial parts of Perralderia coronopifolia were investigated in term of its hepatoprotective effect of female Wistar albino rats (n, 36; average age, 48 ± 5 days; weighing 150 ± 18 g) against PCP (pentachlorphenol)-induced toxicity. PCP (20 mg/kg b.w.) and plant extract (50 mg/kg b.w.) were administered daily by gavages for 2 weeks. Vitamin E (100 mg/kg b.w.) was given intraperitoneally as a positive control. Lipid peroxidation (LPO) levels, reduced glutathione (GSH) levels, and glutathione peroxidase (GPx) activities were evaluated in liver homogenates. While, aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol, and triglyceride parameters were analyzed in serums. The liver fragments were observed using light microscopy. Experimental results exhibited that PCP-treated group has a significant increase in the liver lipid peroxidation (LPO) levels of animals while decreased in plant extract-treated group. In addition, PCP caused significant decreases in glutathione peroxidase (GPx) activities and reduced glutathione (GSH) levels. Moreover, PCP induced hepatotoxicity by increasing serum transaminase enzymes, cholesterol, and triglyceride levels. While, these levels were restored to control value in animals treated with plant extract. The regularized levels of LPO, GSH, cholesterol, triglyceride, transaminase enzymes, and GPx activities revealed the antioxidant properties of the extract plant as well as of the vitamin E. The histological study showed the hepatoprotective effect of our extracts against PCP-induced acute intoxication, protecting the hepatic architecture and decreasing the functional and structural alterations of the liver. The plant extract had high antioxidant potential and completely prevented the toxic effect of PCP on the above of liver and serum parameters.


Perralderia coronopifolia Pentachlorphenol Antioxidant enzymes Lipid peroxidation level Glutathione Hepatotoxicity 


Funding information

This work was supported by the grants from Scientific and Technological Research Council of Turkey (TUBITAK, 114Z683), Ondokuz Mayis University (BAP: PYO.FEN.1904.11.016, Turkey), Cankiri Karatekin University (BAP: FF080515B30, Turkey), and Algerian Ministry of Higher Education.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abhay KP, Sunanda D (2015) Histological changes in liver and kidney of cat fish, Heteropneustes fossilis, exposed to pentachlorophenol (PCP). Plant Archives 15(2):1117–1120Google Scholar
  2. Agha F, Hassannane M, Omara E, Hasan A, El-Toumy S (2013) Protective effect of Punica granatum peel extract against pentachlorophenol-induced oxidative stress, cytogenetic toxicity and hepatic damage in rats. Aust J Basic Appl Sci 7:853–864Google Scholar
  3. Amiot MJ, Riollet C, Landrier JF (2009) Polyphénols et syndrome métabolique. Médecine des maladies Métaboliques 3(5):476–482CrossRefGoogle Scholar
  4. Amrani A, Boubekri N, Lassed S, Zama D, Benayache F, Benayache S (2017) Alcool induced hepatocardiotoxicity and oxidative damage in rats: the protective effect of n-butanol extract of green tea (Camellia sinensis L.). Kuntze Cardiovascular & Hematological Disorders - Drug Targets 17(1):18–23Google Scholar
  5. Basu S (2003) Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients. Toxicology 189:113–127CrossRefGoogle Scholar
  6. Bebe FN, Panemangalore M (2003) Exposure to low doses of endosulfan and chlorpyrifos modifies endogenous antioxidants in tissues of rats. J Environ Sci Heal, Part B 38:349–363CrossRefGoogle Scholar
  7. Bekhouche K, Ozen T, Boussaha S, Koldas S, Yenigun S, Lassed S, Demirtas I, Benayache F, Benayache S, Zama D (2018) Antioxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia. Bangl J Pharmacol 13:82–89CrossRefGoogle Scholar
  8. Bernard BK, Hoberman AM, Brown WR, Ranpuria AK, Christian MS (2002) Oral (gavage) two-generation (one litter per generation) reproduction study of pentachlorophenol (penta) in rats. Int J Toxicol 21:301–318CrossRefGoogle Scholar
  9. Bouabdelli F, Djelloul A, Kaid-Omar Z, Semmoud A, Addou A (2012) Antimicrobial activity of 22 plants used in urolithiasis medicine in western Algeria. Asian Pac J Trop Dis 2:530–535CrossRefGoogle Scholar
  10. Boussaha S, Bekhouche K, Boudjerda A, Leon F, Koldas S, Yaglioglu AS, Demirtas I, Brouard I, Marchioni E, Zama D (2015) Chemical constituents, in vitro antioxidant and antiproliferative activities of Perralderia coronopifolia Coss. subsp. eu-coronopifolia M. var. typica M. extract. Rec Nat Prod 9:312Google Scholar
  11. Carstens CP, Blum JK, Witte I (1990) The role of hydroxyl radicals in tetrachlorohydroquinone induced DNA strand break formation in PM2 DNA and human fibroblasts. Chem-Biol Interact 74:305–314CrossRefGoogle Scholar
  12. Chen J, Jiang J, Zhang F, Yu H, Zhang J (2004) Cytotoxic effects of environmentally relevant chlorophenols on L929 cells and their mechanisms. Cell Biol Toxicol 20:183–196CrossRefGoogle Scholar
  13. Djebbari R, Chemam Y, Boubekri N, Lakroun Z, Kebieche M, Amrani A, Benayache F, Benayache S, Zama D (2017) Chemoprotective potential of Helianthemum confertum against the loss of molecular and functional integrity of the liver cell in doxorubicin-treated rats. Int J Pharmacogn Phytochem Res 9(7):903–910Google Scholar
  14. Dong YL, Zhou PJ, Jiang S-Y, Pan XW, Zhao XH (2009) Induction of oxidative stress and apoptosis by pentachlorophenol in primary cultures of Carassius carassius hepatocytes. Comparative biochemistry and physiology. Toxicol Pharm: CBP 150:179–185Google Scholar
  15. Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77CrossRefGoogle Scholar
  16. Fang Q, Shi X, Zhang L, Wang Q, Wang X, Guo Y, Zhou B (2015) Effect of titanium dioxide nanoparticles on the bioavailability, metabolism, and toxicity of pentachlorophenol in zebrafish larvae. J Hazard Mater 283:897–904CrossRefGoogle Scholar
  17. Fernández FP, Labrador V, Pérez MJM, Hazen MJ (2005) Cytotoxic effects inmammalian Vero cells exposed to pentachlorophenol. Toxicology 210:37–44CrossRefGoogle Scholar
  18. Flohé L, Günzler WA (1984) Assays of glutathione peroxidase, Methods in enzymology. Elsevier, Amsterdam, pp 114–120Google Scholar
  19. Hamdouch A, Ali A, Chebli B, Idrissi H, Lalla M (2017) Chemical constituents and antioxydant activity of the essential oils of Perralderia coronopifolia Cosson subsp. Purpurascens shrub of Oasis of Tata from south east of Morocco. Appl J Environ Eng Sci:3–4Google Scholar
  20. Han ZX, Wang JH, Lv WZ (2009) A battery of tests for ecotoxicological evaluation of pentachlorophenol on common carp. Int J Green Nanotechnol: Biom 1:97–107Google Scholar
  21. Kan HF, Zhao FZ, Zhang XX, Ren HQ, Gao SX (2015) Correlations of gut microbial community shift with hepatic damage and growth inhibition of Carassius auratus induced by pentachlorophenol exposure. Environ. Sci. Technol 49:11894–11902CrossRefGoogle Scholar
  22. Kolac UK, Ustuner MC, Tekin N, Ustuner D, Colak E, Entok E (2017) The anti-inflammatory and antioxidant effects of Salvia officinalis on lipopolysaccharide-induced nflammation in rats. J. Med. Food 20:1193–1200CrossRefGoogle Scholar
  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  24. Lushchak VI (2011) Adaptive response to oxidative stress: Bacteria, fungi, plants and animals. Comp Biochem Physiol Part C: Toxicol Phar- macol 153:175–190Google Scholar
  25. Mehta A, Verma RS, Srivastava N (2009) Chlorpyrifos induced alterations in the levels of hydrogen peroxide, nitrate and nitrite in rat brain and liver. Pestic Biochem Phys 94:55–59CrossRefGoogle Scholar
  26. Peng J, Jianshe W, Nan S, Dongbing W, Jiayin D (2017) Effects of pentachlorophenol on the quail (Coturnix japonica) liver detoxification pathway. Chemosphere 177:44–50CrossRefGoogle Scholar
  27. Sai-Kato K, Umemura T, Takagi A, Hasegawa R, Tanimura A, Kurokawa Y (1995) Pentachlorophenol-induced oxidative DNA damage in mouse liver and protective effect of antioxidants. Food Chem. Toxicol 33:877–882CrossRefGoogle Scholar
  28. Sedighi M, Bahmani M, Asgary S, Beyranvand F, Rafieian-Kopaei M (2017) Volatile components of Centaurea calcitrapa L. and Centaurea sphaerocephala L. ssp. sphaerocephala, two Asteraceae growing wild in Sicily. Flavour Fragr J 21(2):282–285Google Scholar
  29. St. Omer VE, Gadusek F (1987) The acute oral LD50 of technical pentachlorophenol in developing rats. Environ Toxicol Chem 6 (2): 147–149Google Scholar
  30. Tiana L, Caib Q, Wei H (1998) Alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during aging. Free Radical Bio Med 24:1477–1484CrossRefGoogle Scholar
  31. Timbrell JA, Seabra V, Waterfield CJ (1995) The in vivo and in vitro protective properties of taurine. Gen Pharmacol 26:453–462CrossRefGoogle Scholar
  32. Uchiyama M, Mihara M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Bioch 86:271–278CrossRefGoogle Scholar
  33. Umosen AJ, Ambali SF, Ayo JO, Mohammed B, Uchendu C (2012) Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in Wistar rats. Asian Pac J Trop Biomed 2:645–650CrossRefGoogle Scholar
  34. Villena F, Montoya G, Klaasen R, Fleckenstein R, Suwalsky M (1992) Morphological changes on nerves and histopathological effects on liver and kidney of rats by pentachlorophenol (PCP). Comp Biochem Phys C 101(3):53–363Google Scholar
  35. Wang Y, Ho Y, Jeng J, Su H, Lee C (2000) Different cell death mechanisms and gene expression in human cells induced by pentachlorophenol and its major metabolite tetrachlorohydroquinone. Chem Biol Interact 128:173–188CrossRefGoogle Scholar
  36. Wang YJ, Lee CC, Chang WC, Liou HB, Ho YS (2001) Oxidative stress and liver toxicity in rats and human hepatoma cell line induced by pentachlorophenol and its major metabolite tetrachlorohydroquinone. Toxicol Lett 122:157–169CrossRefGoogle Scholar
  37. Wang J, Xu J, Gong X, Yang M, Zhang C, Li M (2019) Biosynthesis, chemistry, and pharmacology of polyphenols from Chinese Salvia species: a review. Molecules J 24:155 23 pCrossRefGoogle Scholar
  38. Wen S, Yali J, Xisheng D, Lili D, Chunsheng L, Jianghua W, Gaofeng Z, Huaidong Z, Liqin Y (2019) Combined effects of pentachlorophenol and its byproduct hexachlorobenzene on endocrine and reproduction in zebrafish. Chemosphere 220:216–226CrossRefGoogle Scholar
  39. Yu LQ, Zhao GF, Feng M, Wen W, Li K, Zhang PW, Peng X, Huo WJ, Zhou HD (2014) Chronic exposure to pentachlorophenol alters thyroid hormones and thyroid hormone pathway mRNAs in zebrafish. Environ Toxicol Chem 33:170–176CrossRefGoogle Scholar
  40. Yuan Y, Wu Q, Shi JS, Chen XP (2015) Advance in studies on hepatoprotective effect of Salvia miltiorrhiza and its main components. Impurrity Chin Tradit Med 40:558–593Google Scholar
  41. Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S, Vlietinck AJ (2007) Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: the protective role of the butanolic extract of Paronychia argentea L. Indian J Pharmacol 39(3):145–150CrossRefGoogle Scholar
  42. Zhou J, Zhong X, Chen L, Yang X (1996) Abnormal expressions of hepatocellular proteins and extracellular matrix in CCl4-induced liver injury in rats. Chinese Med J 109:366–371Google Scholar
  43. Zhou G, Chen Y, Liu S, Yao X, Wang Y (2013) In vitro and in vivo hepatoprotective and antioxidant activity of ethanolic extract from Meconopsis integrifolia (Maxim.) Franch. J Ethnopharmacol 148:664–670CrossRefGoogle Scholar
  44. Zhu BZ, Shan GQ (2009) Potential mechanism for pentachlorophenol-induced carcinogenicity: a novel mechanism for metal-independent production of hydroxyl radicals. Chem Res Toxicol 22:969–977CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Khadidja Bekhouche
    • 1
  • Tevfik Ozen
    • 2
    Email author
  • Sara Boussaha
    • 3
  • Ibrahim Demirtas
    • 4
  • Mounir Kout
    • 5
  • Kemal Yildirim
    • 2
  • Djamila Zama
    • 1
    • 3
  • Fadila Benayache
    • 3
  • Samir Benayache
    • 3
  1. 1.Department of Animal Biology, Faculty of Nature and Life SciencesUniversity Frères Mentouri 1ConstantineAlgeria
  2. 2.Department of Chemistry, Faculty of Science and LettersOndokuz Mayis UniversitySamsunTurkey
  3. 3.Research Unit: Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL)University Frères Mentouri 1ConstantineAlgeria
  4. 4.Plant Research Laboratory, Department of ChemistryUniversity of CankiriKaratekinTurkey
  5. 5.Anatomic and Pathologic Cytology LaboratoryUniversity Hospital CenterConstantineAlgeria

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