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Environmental Science and Pollution Research

, Volume 26, Issue 30, pp 31133–31141 | Cite as

Influence of chlorothalonil and carbendazim fungicides on the transformation processes of urea nitrogen and related microbial populations in soil

  • Hong DingEmail author
  • Xiangzhou Zheng
  • Jin Zhang
  • Yushu Zhang
  • Juhua Yu
  • Deli Chen
Research Article
  • 57 Downloads

Abstract

To improve crop yielding, a large amount of fungicides is continuously applied during the agricultural management, while the effects of fungicides residues on microbial processing of N in soil need further study. In the present study, two broad spectrum fungicides, chlorothalonil and carbendazim, were applied at the rates of 5, 10, and 50 mg of active ingredient (A.I.) per kg of dry soil combined with urea with 200 mg of N per kg of dry soil under laboratory conditions. The results showed that chlorothalonil obviously retarded the hydrolysis of urea, whereas carbendazim accelerated it in 4 days after the treatments (P < 0.05). Chlorothalonil reduced denitrification, nitrification, and N2O production (P < 0.05), but not for carbendazim. Further analysis on N-associated microbial communities showed chlorothalonil reduced nitrosomonas populations at the rates of 10 and 50 mg of A.I. per kg and autotrophic nitrifying bacterial populations at three application rates (P < 0.05), but Carbendazim decreased nitrosomonas populations only at the rate of 50 mg of A.I. per kg and also autotrophic nitrifying bacterial populations at three rates and heterotrophic nitrifying bacterial populations at the rates of 10 and 50 mg of A.I. per kg. The reasons for this difference were ascribed to arrest urea hydrolysis and impediment of denitrification and nitrification processes by chlorothalonil. In conclusion, to improve crop yielding, chlorothalonil might be more beneficial to conserve soil N by improving soil N fertility, compared with carbendazim.

Keywords

Chlorothalonil Carbendazim Soil microflora Nitrogen Fungicides 

Notes

Funding information

This work was supported by grants from the National Natural Science Foundation of China (grant no. 31270556, 41501269), and Research project of Fujian Academy of Agricultural Sciences (STIT2017-1-9).

References

  1. Aponte C, Maranon T, Garcıa LV (2010) Microbial C, N and P in soils of Mediterranean oak forests: influence of season, canopy cover and soil depth. Biogeochemistry 101:77–92CrossRefGoogle Scholar
  2. Baćmaga M, Wyszkowska J, Kucharski J (2018) The influence of chlorothalonil on the activity of soil microorganisms and enzymes. Ecotoxicology 27(9):1188–1202CrossRefGoogle Scholar
  3. Banerjee A, Banerjee AK (1987) Influence of captan on some microorganisms and microbial processes related to the nitrogen cycle. Plant Soil 102:239–245CrossRefGoogle Scholar
  4. Banerjee A, Banerjee AK (1991) Effect of the fungicides tridemorph and vinclozolin on soil microorganisms and nitrogen metabolism. Folia Microbiologica 36:567–571CrossRefGoogle Scholar
  5. Bremner JM (1995) Recent research on problems in the use of urea as a nitrogen fertilizer. Fertilizer Research 42:321–329CrossRefGoogle Scholar
  6. Chen SK, Edwards CA (2001) A microcosm approach to assess the effects of fungicides on soil ecological processes and plant growth: comparisons of two soil types. Soil Biol Biochem 33:1981–1991CrossRefGoogle Scholar
  7. Chen SK, Edwards CA, Subler S (2001) Effects of the fungicides benomyl, captan and chlorothalonil on soil microbial activity and nitrogen dynamics in laboratory incubations. Soil Biol Biochem 33:1971–1980CrossRefGoogle Scholar
  8. Crenshaw CL, Lauber C, Sinsabaugh RL, Stavely LK (2008) Fungal control of nitrous oxide production in semiarid grassland. Biogeochemistry 87:17–27CrossRefGoogle Scholar
  9. Ding H, Li SQ, Zhang YS, Hu XX, Zheng XZ, Zhang J, Weng BQ, Chen DL (2015) The fate of urea nitrogen applied to a vegetable crop rotation system. Nutr Cycl Agroecosyst 103:279–292CrossRefGoogle Scholar
  10. Elliott GC, Lang HJ (1991) Effects of fungicides on nitrogen transformations in soilless potting media. J Amer Soc Hort Sci 116:961–964CrossRefGoogle Scholar
  11. Hansson GB, Klemedtsson L, Stenström J, Torstensson L (1991) Testing the influence of chemicals on soil autotrophic ammonium oxidation. Environ Toxic Water Qual 6:351–360CrossRefGoogle Scholar
  12. Johns D, Williams H, Farrish K, Wagner S (2004) Denitrification and soil characteristics of wetlands created on two mine soils in east Texas, USA. Wetlands 24:57–67CrossRefGoogle Scholar
  13. Johnsen K, Jacobsen CS, Torsvik V, Sørensen J (2001) Pesticide effects on bacterial diversity in agricultural soils – a review. Biol Fertil Soils 33:443–453CrossRefGoogle Scholar
  14. Kidd PS, Prieto-Fernández A, Monterroso C, Acea MJ (2008) Rhizosphere microbial community and hexachlorocyclohexane degradative potential in contrasting plant species. Plant Soil 302:233–247CrossRefGoogle Scholar
  15. Kihn A, Laurent P, Servais P (2000) Measurement of potential activity of fixed nitrifying bacteria in biological filters used in drinking water production. J Indus Microbiol Biotech 24:161–166CrossRefGoogle Scholar
  16. Kinney CA, Mosier AR, Ferrer I, Furlong ET, Mandernack KW (2004) Effects of the fungicides mancozeb and chlorothalonil on fluxes of CO2, N2O, and CH4 in a fertilized Colorado grassland soil. J Geophys Res 109:D05303.  https://doi.org/10.1029/2003JD003655 CrossRefGoogle Scholar
  17. Lang M, Cai Z (2008) Effects of fungicide chlorothalonil on soil nitrous oxide and carbon dioxide emissions. Chinese J Appl Ecol 19:2745–2750Google Scholar
  18. Lang M, Cai Z (2009) Effects of chlorothalonil and carbendazim on nitrification and denitrification in soils. J Environ Sci 21:458–467CrossRefGoogle Scholar
  19. Lin Y, Kong HN, He YL, Yan L, Li CJ (2006) Isolation and characterization of heterotrophic nitrifying bacteria. Environ Sci 27:324–328 (in Chinese with English abstract)Google Scholar
  20. Mulvaney RL, Bremner JM (1979a) A modified diacetyl monoxime method for colorimetric determination of urea in soil extracts. Commun Soil Sci Plana 10:1163–1170CrossRefGoogle Scholar
  21. Mulvaney RL, Bremner JM (1979b) A modified diacetyl monoxime method for colorimetric determination of urea in soil extracts. Commun in Soil Science and Plant Analysis 10(8):1163–1170CrossRefGoogle Scholar
  22. Podio NS, Guzmán CA, Meriles JM (2008) Microbial community structure in a silty clay loam soil after fumigation with three broad spectrum fungicides. J Environ Sci Health, Part B 43:333–340CrossRefGoogle Scholar
  23. Pozo CGroup of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spai;, Rodelas BGroup of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain; Salmeron VGroup of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain; Martinez-Toledo MVGroup of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain; Vela GRGroup of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain; Gonzalez-Lopez JGroup of Nitrogen Fixation, Department of Microbiology, Faculty of Pharmacy and Institute of Water Research, University of Granada, Granada, 18071, Spain (1994) Effects of fungicides maneb and mancozeb on soil microbial populations. Toxicol Environ Chem 43:123–132CrossRefGoogle Scholar
  24. Puglisi E, Vasileiadis S, Demiris K, Bassi D, Karpouzas DG, Capri E, Cocconcelli PS, Trevisan M (2012) Impact of fungicides on the diversity and function of non-target ammonia-oxidizing microorganisms residing in a litter soil cover. Microbial Ecol 64:692–701CrossRefGoogle Scholar
  25. Ramudu AC, G.J Mohiddin, Srinivasulu M, Madakka M, Rangaswamy V (2011) Impact of fungicides chlorothalonil and propiconazole on microbial activities in groundnut (Arachis hypogaea L.) Soils. ISRN Microbiology, ID 623404, doi: https://doi.org/10.5402/2011/623404, 1, 623407
  26. Ramudu AC, Srinivasulu M, Mohiddin GJ, Rangaswamy V (2012) Effect of Fungicides on Urease and Protease Activities in Two Groundnut (Arachis hypogaea L.) Soils. Intern J Environ Protect 2:23–28Google Scholar
  27. Satapute P, Kamble MV, Adhikari SS, Jogaiah S (2019) Influence of triazole pesticides on tillage soil microbial populations and metabolic changes. Sci Total Environ 651:2334–2344CrossRefGoogle Scholar
  28. Soper FM, Groffman PM, Sparks JP (2016) Denitrification in a subtropical, semi-arid North American savanna: field measurements and intact soil core incubations. Biogeochem 128:257–266CrossRefGoogle Scholar
  29. Sudisha J, Niranjana SR, Umesha S, Prakash HS, Shekar Shetty H (2006) Transmission of seed-borne infection of muskmelon by Didymella bryoniae and effect of seed treatments on disease incidence and fruit yield. Biological Control 37:196–205CrossRefGoogle Scholar
  30. Taylor AE, Zeglin LH, Dooley S, Myrold DD, Bottomley PJ (2010) Evidence for different contributions of archaea and bacteria to the ammonia-oxidizing potential of diverse Oregon soils. Appl Environ Microbiol 76:237691–237698CrossRefGoogle Scholar
  31. Tiedje JM, Simkins S, Groffman PM (1989) Perspectives on measurement of denitrification in the field including recommended protocols for acetylene based methods. Plant Soil 115:261–284CrossRefGoogle Scholar
  32. Tu CM (1993) Effect of fungicides, captafol and chlorothalonil, on microbial and enzymatic activities in mineral soil. J Environ Sci Health, Part B 28:67–80CrossRefGoogle Scholar
  33. Tu CM (1994) Effects of fungicides on microbial activities in sandy soil. Intern J Environ Health Res 4:133–140CrossRefGoogle Scholar
  34. Uyanoz R, Cetin U, Karaarslan E (2005) Effect of three fungicides on soil microbial activity and nitrogen dynamics. Paki J Biol Sci 8:805–809CrossRefGoogle Scholar
  35. Wainwright M, Pugh GJF (1973) The effect of three fungicides on nitrification and ammonification in soil. Soil Biol Biochem 5:577–584CrossRefGoogle Scholar
  36. Walia A, Mehta P, Guleria S, Chauhan A, Shirkot CK (2014) Impact of fungicide mancozeb at different application rates on soil microbial populations, soil biological processes, and enzyme activities in soil. The Scientific World J 702909. doi  https://doi.org/10.1155/2014/702909
  37. Wang XG, Song M, Wang YQ, Gao CM, Zhang Q, Chu XQ, Fang H, Yu YL (2012) Response of soil bacterial community to repeated applications of carbendazim. Ecotoxicology and Environmental Safety 75:33–39CrossRefGoogle Scholar
  38. Yan L, Yu M, Zhang J, Li S, Hou L, Qin Z (2013) Effect of three fungicides on diversity of soil bacterial community. J Northeast Agric University 44:29–33Google Scholar
  39. Yang C, Hamel C, Vujanovic V, Gan Y (2011) Fungicide: modes of action and possible impact on nontarget microorganisms. ISRN Ecology, ID 130289, doi: https://doi.org/10.5402/2011/130289, 1, 8
  40. Yeomans JC, Bremner JM (1985) Denitrification in soil: effects of insecticides and fungicides. Soil Biol Biochem 17:453–456CrossRefGoogle Scholar
  41. Yevgeniy M, David PH, Sharon J (2013) Hall Fungi mediate nitrous oxide production but not ammonia oxidation in arid land soils of the southwestern US. Soil Biol Biochem 63:24–36CrossRefGoogle Scholar
  42. Yu Y, Chu X, Pang G, Xiang Y, Fang H (2009) Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil. J Environ Sci 21:179–185CrossRefGoogle Scholar
  43. Yu S, Wages MR, Cobb GP, Maul JD (2013) Effects of chlorothalonil on development and growth of amphibian embryos and larvae. Environ Pollut 181:329–334CrossRefGoogle Scholar
  44. Yu JH, Fan CX, Zhong JC, Zhang L, Zhang L, Wang CH, Yao XL (2016a) Effects of sediment dredging on nitrogen cycling in Lake Taihu, China: insight from mass balance based on a 2-year field study. Environ Sci Pollut Res 23:3871–3883CrossRefGoogle Scholar
  45. Yu JH, Fan CX, Zhong JC, Zhang YL, Wang CH, Zhang L (2016b) Evaluation of in situ simulated dredging to reduce internal nitrogen flux across the sediment-water interface in Lake Taihu, China. Environ Pollut 214:866–877CrossRefGoogle Scholar
  46. Zhang M, Xu Z, Teng Y, Christie P, Wang J, Ren W, Luo Y, Li Z (2016) Non-target effects of repeated chlorothalonil application on soil nitrogen cycling: the key functional gene study. Sci Total Environ 543:636–643CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Soil and FertilizerFujian Academy of Agricultural SciencesFuzhouChina
  2. 2.Faculty of Veterinary and Agricultural Sciencesthe University of MelbourneVictoriaAustralia

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