Improvement of bioethanol production from pomegranate peels via acidic pretreatment and enzymatic hydrolysis

  • Ekin Demiray
  • Sevgi Ertuğrul KaratayEmail author
  • Gönül Dönmez
Research Article


The aim of this study was to improve the ethanol production from pomegranate peels (PPs). Therefore, the effect of enzymatic hydrolysis and different pretreatments on ethanol production by yeasts was examined. There were three different enzyme concentrations (3.6, 7.2, 14.4 FPU/g substrate) tested for enzymatic hydrolysis, and four different PP media, such as WSPP (whole slurry of PP), LFPP (liquid fraction of PP), WSFPP (washed solid fraction of PP) and N-WSFPP (non-washed solid fraction of PP), were prepared. Bioethanol production was monitored for 96 h. Maximum ethanol concentrations were obtained at WSPP medium as 12.69 g/L, 14.35 g/L and 4.23 g/L in Saccharomyces cerevisiae, Kluyveromyces marxianus and Pichia stipitis, respectively. On the other hand, the washing step of biomass increased the kinetic parameters dramatically and the highest theoretical ethanol yields and YP/S values were obtained from WSFPP medium in all tested yeasts. Theoretical ethanol yields were 97.8%, 98.7% and 35.5% for S. cerevisiae, K. marxianus and P. stipitis, respectively. Qp values were observed as 0.98 g/L h, 0.99 g/L h and 0.04 g/L h for the same yeasts. The highest YP/S values were detected as 0.50 g/g for S. cerevisiae, 0.50 g/g for K. marxianus and 0.30 g/g for P. stipitis in the washed pomegranate peel biomass.


Cellulase Lignocellulosic waste Biofuel Whole slurry Washing Liquid hydrolysate 



The authors thank Novozymes, Denmark for providing the CellicCTec2 used in this study.

Funding information

Financial support was provided by the Ankara University Research Foundation. Project Numbers 17L0430007 and 19H0430002.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ekin Demiray
    • 1
  • Sevgi Ertuğrul Karatay
    • 1
    Email author
  • Gönül Dönmez
    • 1
  1. 1.Department of Biology, Faculty of ScienceAnkara UniversityAnkaraTurkey

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