The effects of naringin on different cyclophosphamide-induced organ toxicities in rats: investigation of changes in some metabolic enzyme activities

  • Cuneyt CaglayanEmail author
Research Article


Cyclophosphamide (CYP) is a common anticancer drug used in the treatment of various malignancies. Naringin (NG) is a natural bioflavonoid that have been reported to have many medicinal and pharmacological properties. Acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA), α-glycosidase (α-Gly), and aldose reductase (AR) enzymes are the essential biological molecules needed for metabolic processes in all living cells. In the present study, the aim was to investigate the effect of NG against CYP-induced liver, brain, kidney, heart, and testis toxicities on some metabolic enzyme activities such as AChE, BChE, CA, α-Gly, and AR. Thirty-five male Wistar rats were randomly divided into five groups with each group consisting of seven rats. The rats were subjected to oral treatment of NG (50 and 100 mg/kg body weight) for 7 days before administering a single dose of CYP (200 mg/kg body weight, i.p) on the seventh day. Treatment with NG in all tissues regulated these enzyme activities in CYP-induced rats. The results of this study showed that NG regulates abnormal increases and decreases in CYP-induced metabolic enzyme activities in all tissues.


Acetylcholinesterase Aldose reductase Butyrylcholinesterase Carbonic anhydrase Cyclophosphamide Naringin 



The author thanks Assist. Prof. Yusuf Temel, Prof. Dr. Fatih Mehmet Kandemir, and Prof. Dr. İlhami Gülçin for their valuable contributions to this study.


This work was financially supported by Bingol University, Foundation of Scientific Researches Projects (Project number BAPSSHMYO.2016.00.001).

Compliance with ethical standards

The study was designed and conducted according to ethical norms approved by the Animal Experimentation Ethics Committee of the Bingol University (Bingol, Turkey) (Approval no. 2018-9/2).

Conflict of interest

The author declares that he has no conflict of interest.


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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Veterinary Medicine, Department of BiochemistryBingol UniversityBingolTurkey

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