Self-rooted grafting influences the growth and cadmium accumulation characteristics in the post generation of Cosmos sulphureus

  • Jin Wang
  • Yumei Tan
  • Ming’an Liao
  • Lijin LinEmail author
  • Yi Tang
  • Huifen Zhang
  • Hui Xia
  • Dong Liang
  • Qunxian Deng
  • Xiulan Lv
  • Cheng Chen
  • Wei Ren
Research Article


To determine whether self-rooted grafting increases the cadmium (Cd) accumulation in post generations of hyperaccumulator or accumulator plants, a pot experiment was conducted to study the effects of self-rooted grafting on growth and Cd accumulation in the post generation of the accumulator plant Cosmos sulphureus. Four treatments were applied in the experiment with soil Cd concentration of 5 mg kg−1: ungrafted (UG), self-rooted grafting of the same C. sulphureus seedling (SG), self-rooted grafting of two C. sulphureus seedlings at the same growth stage (TG), and self-rooted grafting of two C. sulphureus seedlings at different developmental stages (DG). Compared with those of UG plants, the SG, TG, and DG treatments increased the root, stem, leaf, and shoot biomasses of plants in the post-grafting generation, consistent with the rank order DG > TG > SG > UG. The SG, TG, and DG treatments decreased the Cd contents in different organs of the post-grafting generation compared with those of UG plants. Only DG increased Cd extraction by the shoots in the post-grafting generation, which was increased by 6.28% compared with that of the UG treatment. In addition, SG, TG, and DG increased the photosynthetic pigment contents and enhanced antioxidant enzyme activities in the post-grafting generation compared with those of the UG treatment. Thus, self-rooted grafting promoted growth of C. sulphureus plants in the post generation. The DG treatment increased Cd extraction by C. sulphureus plants in the post-grafting generation, which may be exploited for phytoremediation of urban Cd-contaminated soil.


Self-rooted grafting Post-grafting generation Cadmium Cosmos sulphureus Accumulator 



We thank Robert McKenzie, PhD, from Liwen Bianji, Edanz Group China (, for editing the English text of a draft of this manuscript.


  1. Datta R, Sarkar D (2004) Effective integration of soil chemistry and plant molecular biology in phytoremediation of metals: an overview. Environ Geosci 11(2):53–63CrossRefGoogle Scholar
  2. Hao ZB, Cang J, Xu Z (2004) Plant physiology experiment. Harbin Institute of Technology Press. Harbin, ChinaGoogle Scholar
  3. Hu YQ, Su Y, Han FY, Shu ML, Cui SM (2007) The research of anatomical structures and three activity of antioxidases change of grafted cucumber seedling. J Inner Mongolia Agr Univ (Nat Sci Edit) 28:224–230Google Scholar
  4. Ji WD, Shi GX, Zhang H, Xu QS, Xu Y, Du KH (2007) Physiological and ultrastructural responses of Potamogeton crispus to Hg2+ stress. Acta Ecol Sin 27(7):2856–2863Google Scholar
  5. Lin LJ, Luo L, Zhang X, Yang DY, Liao MA, Tang FY (2015) Effects of rape rootstock on cadmium accumulation characteristics of Capsella bursa-pastoris post-grafting generation. Acta Agric Boreali-Sinica 30(1):207–212Google Scholar
  6. Lin LJ, Ma QQ, Shi J, He J, Zhong CC, Huang JJ, Wen K, Liao MA (2016a) Study on cadmium accumulation characteristics of flower plant Cosmos sulphureus. J Soil Water Conserv 30(3):141–146Google Scholar
  7. Lin L, Yang D, Wang X, Liao M, Wang Z, Lv X, Tang F, Liang D, Xia H, Lai Y, Tang Y (2016b) Effects of grafting on the cadmium accumulation characteristics of the potential Cd-hyperaccumulator Solanum photeinocarpum. Environ Monit Assess 188(2):82CrossRefGoogle Scholar
  8. Liu L, Ma Q, Lin L, Tang Y, Wang J, Lv X, Liao M, Xia H, Chen S, Li J, Wang X, Lai Y, Liang D (2017) Effects of exogenous abscisic acid on cadmium accumulation in two ecotypes of hyperaccumulator Bidens pilosa. Environ Prog Sustain Energy 36(6):1643–1649CrossRefGoogle Scholar
  9. Liu YS (2001) The theoretical and practical significance of plant distant grafting in ancient and modern China. Stud Hist Nat Sci 20(4):352–361Google Scholar
  10. Liu YS, Wang QL, Li BY (2010) New insights into plant graft hybridization. Heredity 104(1):1–2CrossRefGoogle Scholar
  11. Liu Y, Lin L, Jin Q, Zhu X (2015) Cadmium accumulation and tolerance in the Cd-accumulator Capsella bursa-pastoris. Environ Prog Sustain Energy 34(3):663–668CrossRefGoogle Scholar
  12. Liu ZL, He XY, Chen W (2013) Lonicera japonica Thunb. — a newly discovered cadmium hyperaccumulator. Ecol Environ Sci 22(4):666–670Google Scholar
  13. Luo YM (1995) Phytoremediation of metal contaminated soil. Soils 31(5):261–265 280Google Scholar
  14. Nie F (2005) New comprehensions of hyperaccumulator. Ecol Environ 14:136–138Google Scholar
  15. Nie LC, Chen GL, Zhao LL (1999) Study on growth and development characteristics of watermelon grafted seedlings. China Watermelon and Muskmelon 12(1):7–10Google Scholar
  16. Pan XW, Sun XH, Zhang FY, Zhao C, Zhang XS, Du WG (2012) Optimization of distant grafting mutagenesis technology in soybean. Soybean Science 31(2):237–241Google Scholar
  17. Rastmanesh F, Moore F, Keshavarzi B (2010) Speciation and phytoavailability of heavy metals in contaminated soils in Sarcheshmeh area, Kerman Province, Iran. Bull Environ Contam Toxicol 85:515–519CrossRefGoogle Scholar
  18. Rouphael Y, Schwarz D, Krumbein A, Colla G (2010) Impact of grafting on product quality of fruit vegetables. Sci Hortic 127:172–179CrossRefGoogle Scholar
  19. Tessier A, Campbell PGC, Bisson M (1979) Sequential extraction procedure for the speciation of particulate trace metals. Anal Chem 51:844–851CrossRefGoogle Scholar
  20. Türkan I, Bor M, Özdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231CrossRefGoogle Scholar
  21. Wang H, Shi J, Lin L, Yang D, Huang K, Zhang X (2016a) Effects of mulching with tolerant plant straws on growth and cadmium accumulation of Capsella bursa-pastoris in cadmium contaminated soil. Bullf Soil Water Conserv 36(1):184–187Google Scholar
  22. Wang J, Lin LJ, Liu L, Liang D, Xia H, Lv XL, Liao MA, Wang ZH, Lai YS, Tang Y, Wang X, Ren W (2016b) Interspecies rootstocks affect cadmium accumulation in post grafting generation plants of potential cadmium-hyperaccumulator Solanum photeinocarpum. Environ Toxicol Chem 35(11):2845–2850CrossRefGoogle Scholar
  23. Wei SH, Zhou QX, Wang X, Zhang KS, Guo GL, Ma Lena QY (2005) A newly-discovered Cd-hyperaccumulator Solanum nigrum L. Chin Sci Bull 50(1):33–38CrossRefGoogle Scholar
  24. Wu R, Wang X, Lin Y, Ma Y, Liu G, Yu X, Zhong S, Liu B (2013) Inter-species grafting caused extensive and heritable alterations of DNA methylation in Solanaceae plants. PLoS One 8(4):e61995CrossRefGoogle Scholar
  25. Yi YL, Wang Y, Zhang DG, Guo LL, Qi X (2010) Analysis on distribution and migration characteristics of Pb and Cd in soil, plant and dust fall in urban region of Shenyang city. Chin J Soil Sci 41(6):1466–1470Google Scholar
  26. Zhang CL, Zhang YD, Lv LW, Zhang JC (1995) On the potassium uptake of the graft plants of different varieties of tomato. J Nanjing Agr Univ 18(3):72–80Google Scholar
  27. Zhang SR, Lin HC, Deng LJ, Gong GS, Jia YX, Xu XX, Li T, Li Y, Chen H (2013) Cadmium tolerance and accumulation characteristics of Siegesbeckia orientalis L. Ecol Eng 51:133–139CrossRefGoogle Scholar
  28. Zhang XF, Xia HP, Li ZA, Zhuang P, Gao B (2011) Identification of a new potential Cd-hyperaccumulator Solanum photeinocarpum by soil seed bank-metal concentration gradient method. J Hazard Mater 189:414–419CrossRefGoogle Scholar
  29. Zhang XL (2002) Grafting technology and plant improvement. J Xingtai Vocat and Tech Coll 19(2):71–71Google Scholar
  30. Zhao ZY (2013) Preliminary study on establishment of grafting system of different plants and grafting induced variation mechanism (thesis of master). Henan Institute of Science and Technology. Zhengzhou, ChinaGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Pomology and OlericultureSichuan Agricultural UniversityChengduChina
  2. 2.College of HorticultureSichuan Agricultural UniversityChengduChina
  3. 3.College of EconomicsSichuan Agricultural UniversityChengduChina
  4. 4.Maize Research InstituteNeijiang Academy of Agricultural SciencesNeijiangChina

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