Contributions of egg production and egg hatching to the total toxicity of teflubenzuron in Yuukianura szeptyckii (Collembola) in soil toxicity test

  • Yun-Sik Lee
  • Jino Son
  • June Wee
  • Yongeun Kim
  • Du Yung Kim
  • Jung-Hwan Kwon
  • Kijong ChoEmail author
Research Article


In the standard ISO soil toxicity test using Collembola, adult survival and juvenile production are the only endpoints that can be attainable. The information on egg production and egg hatching cannot be investigated in the ISO test. To overcome this limitation, in this study, the effects of teflubenzuron on life history parameters of Yuukianura szeptyckii (Collembola) were investigated with a compressed soil test. Teflubenzuron is an insect growth regulator and has a negative effect on egg production, and egg hatching process of arthropods. LC50 decreased with increases in exposure period from 6.97 mg/kg in the third week to 3.60 mg/kg in the fourth week. The EC50 for egg and juvenile production was 0.57 mg/kg and 0.26 mg/kg, respectively. The hatching rate decreased significantly from 46 to 7% as the concentration increased from 0.25 to 1.00 mg/kg, respectively, and the molting frequency was significantly affected only at > 4 mg/kg. The toxic contribution rate (TCR) was defined as the ratio of juvenile production at an exposure concentration compared with the control, and a simple life history model was developed for TCR estimations. At the lower concentrations (< 0.3 mg/kg), the hatching rate reduction was a main contributor to the total toxicity, but the adult mortality and egg production reduction were the main contributors at the higher concentrations (> 2.0 mg/kg). The contribution of egg production reduction remained relatively constant. Since collembolan populations in the soil can be composed of various developmental stages, the differences in the sensitivity to chemicals depending on the developmental stages should be included in the assessment of the toxic impact on soil ecosystems.


IGRs ISO Compressed soil test Life history Toxic contribution rate 


Funding information

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (NRF-2016R1A2B4015651) and the Korea University Grant to K. Cho.

Supplementary material

11356_2019_5892_MOESM1_ESM.docx (308 kb)
ESM 1 (DOCX 308 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Ojeong Eco-Resilience InstituteKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Environmental Science and Ecological EngineeringKorea UniversitySeoulRepublic of Korea
  3. 3.Institute of Environment and Ecology, OJERIKorea UniversitySeoulRepublic of Korea

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