Environmental Science and Pollution Research

, Volume 26, Issue 26, pp 27063–27072 | Cite as

Multi-wavelength spectrophotometric determination of hydrogen peroxide in water by oxidative coloration of ABTS via Fenton reaction

  • Mengyun Wang
  • Daiyao Wang
  • Shiyi Qiu
  • Junyang Xiao
  • Huahua Cai
  • Jing ZouEmail author
Research Article


In this study, a sensitive and low-cost multi-wavelength spectrophotometric method for the determination of hydrogen peroxide (H2O2) in water was established. The method was based on the oxidative coloration of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) via Fenton reaction, which resulted in the formation of green radical (ABTS•+) with absorbance at four different wavelengths (i.e., 415 nm, 650 nm, 732 nm, and 820 nm). Under the optimized conditions (CABTS = 2.0 mM, CFe2+ = 1.0 mM, pH = 2.60 ± 0.02, and reaction time (t) = 1 min), the absorbance of the generated ABTS•+ at 415 nm, 650 nm, 732 nm, and 820 nm were well linear with H2O2 concentrations in the range of 0–40 μM (R2 > 0.999) and the sensitivities of the proposed Fenton-ABTS method were calculated as 4.19 × 104 M–1 cm–1,1.73 × 104 M–1 cm–1, 2.18 × 104 M–1 cm–1, and 1.96 × 104 M–1 cm–1, respectively. Meanwhile, the detection limits of the Fenton-ABTS method at 415 nm, 650 nm, 732 nm, and 820 nm were respectively calculated to be 0.18 μM, 0.12 μM, 0.10 μM, and 0.11 μM. The absorbance of the generated ABTS•+ in ultrapure water, underground water, and reservoir water was quite stable within 30 min. Moreover, the proposed Fenton-ABTS method could be used for monitoring the variations of H2O2 concentration during the oxidative decolorization of RhB in alkali-activated H2O2 system.


H2O2 Fe2+ ABTS Multi-wavelength Spectrophotometric method Fenton 


Funding information

This research was supported by the National Natural Science Foundation of China (No. 51708231), China Postdoctoral Science Foundation (No. 2017M612120), Natural Science Foundation of Fujian province (No. 14185013), and Promotion Program for Young and Middle-aged Teacher in Science and Technology Research of Huaqiao University (No. ZQN-YX506).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Municipal and Environmental Engineering, College of Civil EngineeringHuaqiao UniversityXiamenPeople’s Republic of China
  2. 2.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinPeople’s Republic of China

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