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Environmental Science and Pollution Research

, Volume 26, Issue 26, pp 26939–26946 | Cite as

Biogenic AgNPs synthesized via endophytic bacteria and its biological applications

  • M. Shariq Ahmed
  • Ranjani Soundhararajan
  • Tahira Akther
  • Mohammad Kashif
  • Jasim Khan
  • Mohammad Waseem
  • Hemalatha SrinivasanEmail author
Research Article
  • 77 Downloads

Abstract

In this present study, the endophytic bacteria were isolated from the drought-tolerant ornamental plant Pennisetum setaceum. The biomass of endophytic bacteria was utilized for the biogenic synthesis of silver nanoparticles (AgNPs). The synthesis of AgNPs was confirmed by UV-Visible and FTIR spectroscopy followed by SEM analysis. The antibacterial studies were performed through MIC, MBC, and biofilm assays. Efficacy of AgNPs against the human breast cancer (MCF-7) cells was also tested, and the IC50 was determined by MTT assay. In our study, we have observed that the synthesized AgNPs exhibited a dose-dependent cytotoxicity (1–100 μg/mL) against MCF-7 cells and morphological alterations of the cells were also visualized and the IC50 was observed at 50 μg/mL. The treatment of synthesized AgNPs altered the expression of apoptotic proteins including Bax, Bcl-2, and inflammatory marker COX-2 in MCF-7 cells. To the best of our knowledge, this is the first report that demonstrates the AgNPs from endophytic bacteria isolated from the plant Pennisetum setaceum can induce apoptosis in human breast cancer MCF-7 cells. Our results suggest that AgNPs used in this study can be utilized to control human pathogens and can also be utilized to induce apoptosis in breast cancer cells.

Keywords

Endophytic bacteria Apoptosis MCF-7 cells Bcl-2 Bax Silver nanoparticles 

Abbreviations

AgNPs

Silver nanoparticles

FTIR

Fourier transform infrared spectroscopy

SEM

Scanning electron microscopy

MIC

Minimum inhibitory concentration

MBC

Minimum bactericidal concentration

Notes

Acknowledgments

MSA is thankful to the B.S. Abdur Rahman Institute of Science & Technology, Chennai, for providing Junior Research Fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • M. Shariq Ahmed
    • 1
  • Ranjani Soundhararajan
    • 1
  • Tahira Akther
    • 1
  • Mohammad Kashif
    • 2
  • Jasim Khan
    • 2
  • Mohammad Waseem
    • 3
  • Hemalatha Srinivasan
    • 1
    Email author
  1. 1.School of Life SciencesBS Abdur Rahman Crescent Institute of Science & TechnologyChennaiIndia
  2. 2.Department of ToxicologyJamia HamdardNew DelhiIndia
  3. 3.School of MedicineUniversity of BirminghamBirminghamUSA

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