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Environmental Science and Pollution Research

, Volume 26, Issue 25, pp 25844–25854 | Cite as

Ginkgo biloba mitigates silver nanoparticles-induced hepatotoxicity in Wistar rats via improvement of mitochondrial biogenesis and antioxidant status

  • Eman M. Abd El-Maksoud
  • Mohamed A. LebdaEmail author
  • Aml E. Hashem
  • Nabil M. Taha
  • Maher A. Kamel
Research Article
  • 194 Downloads

Abstract

Silver nanoparticles (AgNPs) are noble metal nanoparticles, due to their good physicochemical properties, which have been exploited in biological applications. Nanotechnological applications advance very quickly while few literatures assessed the effects of natural products on the risks of nanoparticles in vivo. Thirty male adult rats were enrolled equally into: control, AgNPs (50 mg/kg b.w i.p 3 times/week) and GBE (100 mg/kg b.w daily per os)+AgNPs. After 30 days, the assessment of liver function, antioxidative status, mitochondrial biogenesis, and histopathological analyses were performed. AgNP exposure enhanced the hepatic lipid peroxidation (+ 281.7%) along with a decline in the reduced glutathione (− 58.3%) levels. The apparent hepatic oxidative damage was associated with obvious hepatic dysfunction that was ascertained by alteration of serum liver enzymatic biomarkers, lipid profile, and pathological hepatic lesions. Following AgNP exposure, hepatic silver and calcium contents were increased without changes in the trace element concentrations. Finally, the mRNA transcripts of hepatic PGC-1α, mtTFA, and Nrf2 were downregulated after AgNP exposure. Interestingly, GBE has the ability to alleviate AgNP-induced hepatic damage assessed by augmentation of reduced glutathione level and mitochondrial biogenesis. This study explored the potential protective role of GBE on AgNPs-induced hepatotoxicity via attenuation of oxidative stress, substantial enhancement of cell viability with concomitant mitigating DNA damage, and mitochondrial dysfunction.

Keywords

Silver nanoparticles Ginkgo biloba Hepatotoxicity Necrosis Oxidative stress Mitochondrial biogenesis Glutathione 

Abbreviations

AgNPs

silver nanoparticles

Akt

protein kinase B pathway

ALT

alanine aminotransferase

ALP

alkaline phosphatase

ANOVA

analysis of variance

ARE

antioxidant response element

AST

aspartate aminotransferase

ATP

adenosine triphosphate

CCl4

carbon tetrachloride

ER

endoplasmic reticulum

GBE

Ginkgo biloba L. extract

GSH

reduced glutathione

GSSG

oxidized glutathione

HCl

hydrochloric acid

HDL-c

high-density lipoprotein cholesterol

HE

hematoxylin and eosin

HNO3

nitric acid

ICP-MS

inductively coupled plasma mass spectrometry

LDL-c

low-density lipoprotein cholesterol

MDA

malondialdehyde

mtTFA

mitochondrial transcription factor A

Nrf2

nuclear factor erythroid 2 like factor

PGC-1α

peroxisome proliferative activated receptor gamma coactivators 1 alpha

ROS

reactive oxygen species

SE

standard error

TAG

Triacylglycerol

TEM

transmission electron microscope

Notes

Compliance with ethical standards

The handling of rats were performed according to international ethical guidelines for the care and use of laboratory animals and the approval of experimental procedures were done by the Experimental Animal Use and Ethics Committee at the Faculty of Veterinary Medicine, Alexandria University, Egypt.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Eman M. Abd El-Maksoud
    • 1
  • Mohamed A. Lebda
    • 1
    Email author
  • Aml E. Hashem
    • 1
  • Nabil M. Taha
    • 1
  • Maher A. Kamel
    • 2
  1. 1.Department of Biochemistry, Faculty of Veterinary MedicineAlexandria UniversityAlexandriaEgypt
  2. 2.Department of Biochemistry, Institute of Medical ResearchAlexandria UniversityAlexandriaEgypt

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