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Assessing interactions, predicting function, and increasing degradation potential of a PAH-degrading bacterial consortium by effect of an inoculant strain

  • Marianela Macchi
  • Sabrina Festa
  • Nelson E. Vega-Vela
  • Irma S. Morelli
  • Bibiana M. CoppotelliEmail author
Research Article

Abstract

A natural phenanthrene-degrading consortium CON was inoculated with an exogenous strain Sphingobium sp. (ex Sp. paucimobilis) 20006FA yielding the consortium called I-CON, in order to study ecological interactions into the bacterial community. DGGE and proteomic profiles and analyses by HTS (High-Throughput Sequencing) technologies demonstrated inoculant establishment and changes on CON composition. Inoculation increased degradation efficiency in I-CON and prevented intermediate HNA accumulation. This could be explained not only by the inoculation, but also by enrichment in Achromobacter genus at expense of a decrease in Klebsiella genus. After inoculation, cooperation between Sphingobium and Achromobacter genera were improved, thereby, some competition could have been generated, and as a consequence, species in minor proportion (cheaters), as Inquilinus sp. and Luteibacter sp., were not detected. Sequences of Sphingobium (corresponding to the inoculated strain) did not vary. PICRUSt predicted a network with bacterial phylotypes connected with enzymes, showing functional redundancy in the phenanthrene pathway, with exception of the first enzymes biphenyl-2,3-diol 1,2-dioxygenase and protocatechuate 4,5-dioxygenase that were only encoded in Sphingobium sp. This is the first report where a natural consortium that has been characterized by HTS technologies is inoculated with an exogenous strain in order to study competitiveness and interactions.

Keywords

Inoculation/invasion Bacterial consortia Degradation potential Invasion Predicted functions PICRUSt Proteome 

Notes

Funding

This research was partially supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT 2013–0103). Festa S. and Macchi M. have doctoral and postdoctoral fellowships supported by CONICET. Morelli I.S. is a research member of CIC-PBA. Coppotelli B.M. is a research member of CONICET.

Supplementary material

11356_2019_5760_MOESM1_ESM.docx (699 kb)
ESM 1 (DOCX 699 kb)

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Authors and Affiliations

  1. 1.Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP; CCT-La Plata, CONICET)La PlataArgentina
  2. 2.Pontificia Universidad JaverianaBogotáColombia
  3. 3.Universidad de Bogotá Jorge Tadeo LozanoBogotáColombia
  4. 4.Comisión de Investigaciones Científicas de la Provincia de Buenos AiresLa PlataArgentina

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