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Effects of food-borne ZnO nanoparticles on intestinal microbiota of common carp (Cyprinus carpio L.)

  • Latifeh ChupaniEmail author
  • Jiri Barta
  • Eliska Zuskova
Research Article

Abstract

Ingestion of nanoparticles (NPs) with antimicrobial properties may disrupt the balance of intestinal microbiota. To investigate the effects of zinc oxide (ZnO) NPs on intestinal flora, common carp Cyprinus carpio were fed a commercial feed containing 500 mg kg−1 ZnO NPs for 6 weeks and compared to a control group receiving a similar feed-only regime. Sequencing data were analyzed both in individual fish and in pooled samples. Sequencing of 16S rRNA encoding gene of individual specimens revealed high variation in intestinal microbial composition. Assessment of pooled results can obscure high individual variation in data. ZnO NPs consumption was not associated with a significant difference in the intestinal microbial community compared to untreated controls. Our results indicated a high individual variation in the intestinal microbiome, which may further point out the importance of functional study over microbial composition to address nanomaterials-microbiome relationship.

Keywords

ZnO Nanoparticles Microbiome Common carp Intestine 

Notes

Acknowledgments

We thank Sarah M. Owens and Stephanie Moormann Greenwald, Biosciences Division, Argonne National Laboratory, USA, for assistance in sequencing analysis. Our appreciation is extended to the Lucidus Consultancy, for the English edition of the manuscript.

Funding information

This research was supported by the Ministry of Education, Youth, and Sports of the Czech Republic projects CENAKVA (LM2018099) and the project Sustainable production of healthy fish in various aquaculture systems; PROFISH (CZ.02.1.01/0.0/0.0/16 019 /0000869).

Supplementary material

11356_2019_5616_MOESM1_ESM.docx (624 kb)
Online Resource 1 Alpha diversity analysis. Rarefaction curves of (A) Chao 1 index, (B) Observed OTUs (DOCX 623 kb)
11356_2019_5616_MOESM2_ESM.xlsx (24 kb)
ESM 2 (XLSX 23 kb)
11356_2019_5616_MOESM3_ESM.xlsx (134 kb)
ESM 3 (XLSX 133 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of HydrocenosesUniversity of South Bohemia in České BudějoviceVodňanyCzech Republic
  2. 2.Department of Ecosystem Biology, Faculty of ScienceUniversity of South Bohemia in České BudějoviceČeské BudějoviceCzech Republic

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