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Nitrous oxide emission mitigation during low–carbon source wastewater treatment: effect of external carbon source supply strategy

  • Hongxiang ChaiEmail author
  • Siping Deng
  • Xiaoyuan Zhou
  • Chuanrong Su
  • Yu Xiang
  • Yan Yang
  • Zhiyu Shao
  • Li Gu
  • Xuan Xu
  • Fangying Ji
  • Qiang He
Research Article
  • 60 Downloads

Abstract

Nitrous oxide (N2O) generated during biological nitrogen removal in wastewater treatment processes has contributed an important proportion to the global warming effect. To evaluate the possibility of N2O emission mitigating by changing carbon source supply strategies, nitrogen transformation characteristics and N2O emissions with methanol one-time dosing and step dosing were investigated. Two sets of laboratory-scale sequencing batch biofilm reactors (SBBRs) were conducted to treat real domestic wastewater with low carbon source. The results revealed that reactors with methanol step dosing showed a lower N2O emission of 0.0402 ± 0.0016 mg/(L·h), together with a higher total nitrogen and ammonia nitrogen removal efficiencies of 83.30% ± 1.21 and 93.45% ± 1.20, respectively. While N2O emission from conventional one-time dosing reactors was 0.0741 ± 0.0025 mg/(L·h), total nitrogen and ammonia nitrogen removal efficiencies were 75.71% ± 0.54 and 88.45% ± 0.59, respectively. The N2O emission factor of SBBR was reduced from 6.26% ± 0.21 to 3.40% ± 0.14 with methanol step dosing. Moreover, nitrification rates in aerobic phases were reduced, while denitrification rates in anoxic phases were elevated. Hence, carbon source step dosing enhanced nitrogen removal and reduced N2O emission compared with one-time dosing, which is a simply achievable strategy for N2O emission reduction in highly automated systems like wastewater treatment plants.

Keywords

Wastewater treatment Nitrous oxide Carbon source Sequencing batch biofilm reactor Nitrogen removal Methanol 

Notes

Funding information

The work reported here was financially supported by the National Key Technology R&D Program of China (2011BAJ07B03).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2019_5516_MOESM1_ESM.docx (33 kb)
ESM 1 (DOCX 32 kb)

References

  1. Adouani N, Lendormi T, Limousy L, Sire O (2010) Effect of the carbon source on N2O emissions during biological denitrification. Resour Conserv Recycl 54:299–302.  https://doi.org/10.1016/j.resconrec.2009.07.011 CrossRefGoogle Scholar
  2. Adouani N, Limousy L, Lendormi T, Sire O (2015) N2O and NO emissions during wastewater denitrification step: influence of temperature on the biological process. Comptes Rendus Chimie 18:15–22.  https://doi.org/10.1016/j.crci.2014.11.005 CrossRefGoogle Scholar
  3. Ahn JH, Kim S, Park H, Rahm B, Pagilla K, Chandran K (2010) N2O emissions from activated sludge processes, 2008-2009: results of a national monitoring survey in the United States. Environ Sci Technol 44:4505–4511.  https://doi.org/10.1021/es903845y CrossRefGoogle Scholar
  4. Antoniou P, Hamilton J, Koopman B, Jain R, Holloway B, Lyberatos G, Svoronos SA (1990) Effect of temperature and pH on the effective maximum specific growth-rate of nitrifying bacteria. Water Res 24:97–101.  https://doi.org/10.1016/0043-1354(90)90070-M CrossRefGoogle Scholar
  5. APHA (2005) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), WashingtonGoogle Scholar
  6. Chai H, Shen Y, Su C et al (2017) Nitrogen removal and nitrous oxide emission on sequencing batch biofilm reactor at different C/N ratio. Fresenius Environ Bull 26:6712–6719Google Scholar
  7. Chandran K, Stein LY, Klotz MG, van Loosdrecht MCM (2011) Nitrous oxide production by lithotrophic ammonia-oxidizing bacteria and implications for engineered nitrogen-removal systems. Biochem Soc Trans 39:1832–1837.  https://doi.org/10.1042/BST20110717 CrossRefGoogle Scholar
  8. Chiou RH, Yang YR (2008) An evaluation of the phosphorus storage capacity of an anaerobic/aerobic sequential batch biofilm reactor. Bioresour Technol 99:4408–4413.  https://doi.org/10.1016/j.biortech.2007.08.038 CrossRefGoogle Scholar
  9. Colliver BB, Stephenson T (2000) Production of nitrogen oxide and dinitrogen oxide by autotrophic nitrifiers. Biotechnol Adv 18:219–232.  https://doi.org/10.1016/s0734-9750(00)00035-5 CrossRefGoogle Scholar
  10. Daelman MRJ, van Voorthuizen EM, van Dongen LGJM, Volcke EIP, van Loosdrecht MCM (2013) Methane and nitrous oxide emissions from municipal wastewater treatment - results from a long-term study. Water Sci Technol 67:2350–2355.  https://doi.org/10.2166/wst.2013.109 CrossRefGoogle Scholar
  11. Desloover J, Vlaeminck SE, Clauwaert P, Verstraete W, Boon N (2012) Strategies to mitigate N2O emissions from biological nitrogen removal systems. Curr Opin Microbiol 23:474–482.  https://doi.org/10.1016/j.copbio.2011.12.030 Google Scholar
  12. Ding DH, Feng CP, Jin YX (2010) Effect of C/N ratio on nitrogen removal in a novel sequencing batch biofilm reactor. International Conference on Bioinformatics & Biomedical Engineering. IEEE. doi: 0.1109/ICBBE.2010.5517614Google Scholar
  13. Frijns J, Roorda J, Mulder M (2008) Op weg naar een klimaatneutrale waterketen. H2O 41(10): 36–37Google Scholar
  14. Frison N, Chiumenti A, Katsou E, Malamis S, Bolzonella D, Fatone F (2015) Mitigating off-gas emissions in the biological nitrogen removal via nitrite process treating anaerobic effluents. J Clean Prod 93:126–133.  https://doi.org/10.1016/j.jclepro.2015.01.017 CrossRefGoogle Scholar
  15. Grommen R, Verhaege M, Verstraete W (2006) Removal of nitrate in aquaria by means of electrochemically generated hydrogen gas as electron donor for biological denitrification. Aquac Eng 34:33–39.  https://doi.org/10.1016/j.aquaeng.2005.03.007 CrossRefGoogle Scholar
  16. Hamlin HJ, MichaelS JT, Beaulaton CM et al (2008) Comparing denitrification rates and carbon sources in commercial scale upflow denitrification biological filters in aquaculture. Aquac Eng 38:79–92.  https://doi.org/10.1016/j.aquaeng.2007.11.003 CrossRefGoogle Scholar
  17. Hu Z, Zhang J, Li S, Xie H (2013) Impact of carbon source on nitrous oxide emission from anoxic/oxic biological nitrogen removal process and identification of its emission sources. Environ Sci Pollut Res 20:1059–1069.  https://doi.org/10.1007/s11356-012-1018-6 CrossRefGoogle Scholar
  18. IPCC (2014) Climate change 2014: mitigation of climate change. In: Contribution of working group iii contribution to the fifth assessment report of the intergovernmental panel on climate change. Cambridge University Press, CambridgeGoogle Scholar
  19. Jia W, Zhang J, Xie H, Yan Y, Wang J, Zhao Y, Xu X (2012) Effect of PHB and oxygen uptake rate on nitrous oxide emission during simultaneous nitrification denitrification process. Bioresour Technol 113:232–238.  https://doi.org/10.1016/j.biortech.2011.10.095 CrossRefGoogle Scholar
  20. Jin Y, Li X (2012) Nitrogen and phosphorus removal in synthetic domestic wastewater using SBBR technology. Appl Mech Mater 209-211:1906–1909.  https://doi.org/10.4028/www.scientific.net/AMM.209-211.1906 CrossRefGoogle Scholar
  21. Kampschreur MJ, Tan NCG, Kleerebezem R, Picioreanu C, Jetten MSM, MCM L (2008a) Effect of dynamic process conditions on nitrogen oxides emission from a nitrifying culture. Environ Sci Technol 42:429–435.  https://doi.org/10.1021/es071667p CrossRefGoogle Scholar
  22. Kampschreur MJ, van der Star WRL, Wielders HA, Mulder JW, Jetten MSM, van Loosdrecht MCM (2008b) Dynamics of nitric oxide and nitrous oxide emission during full-scale reject water treatment. Water Res 42:812–826.  https://doi.org/10.1016/j.watres.2007.08.022 CrossRefGoogle Scholar
  23. Kampschreur MJ, Temmink H, Kleerebezem R, Jetten MSM, van Loosdrecht MCM (2009) Nitrous oxide emission during wastewater treatment. Water Res 43:4093–4103.  https://doi.org/10.1016/j.watres.2009.03.001 CrossRefGoogle Scholar
  24. Khanitchaidecha W, Nakaruk A, Koshy P, Futaba K (2015) Comparison of simultaneous nitrification and denitrification for three different reactors. Biomed Res Int.  https://doi.org/10.1155/2015/901508
  25. Kim SW, Miyahara M, Fushinobu S, Wakagi T, Shoun H (2010) Nitrous oxide emission from nitrifying activated sludge dependent on denitrification by ammonia-oxidizing bacteria. Bioresour Technol 101:3958–3963.  https://doi.org/10.1016/j.biortech.2010.01.030 CrossRefGoogle Scholar
  26. Kishida N, Kim JH, Kimochi Y, Nishimura O, Sasaki H, Sudo R (2004) Effect of C/N ratio on nitrous oxide emission from swine wastewater treatment process. Water Sci Technol 49:359–365.  https://doi.org/10.2166/wst.2004.0775 CrossRefGoogle Scholar
  27. Kong HN, Kimochi Y, Mizuochi M et al (2002) Study of the characteristics of CH4 and N2O emission and methods of controlling their emission in the soil-trench wastewater treatment process. Sci Total Environ 290:59–67.  https://doi.org/10.1016/s0048-9697(01)01058-0 CrossRefGoogle Scholar
  28. Kong Q, Liang S, Zhang J, Xie H, Miao M, Tian L (2013) N2O emission in a partial nitrification system: dynamic emission characteristics and the ammonium-oxidizing bacteria community. Bioresour Technol 127:400–406.  https://doi.org/10.1016/j.biortech.2012.10.011 CrossRefGoogle Scholar
  29. Law Y, Lant P, Yuan Z (2011) The effect of pH on N2O production under aerobic conditions in a partial nitritation system. Water Res 45:5934–5944.  https://doi.org/10.1016/j.watres.2011.08.055 CrossRefGoogle Scholar
  30. Lemaire R, Meyer R, Taske A, Crocetti GR, Keller J, Yuan Z (2006) Identifying causes for N2O accumulation in a lab-scale sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal. J Biotechnol 122:62–72.  https://doi.org/10.1016/j.jbiotec.2005.08.024 CrossRefGoogle Scholar
  31. Lew B, Stief P, Beliavski M, Ashkenazi A, Svitlica O, Khan A, Tarre S, de Beer D, Green M (2012) Characterization of denitrifying granular sludge with and without the addition of external carbon source. Bioresour Technol 124:413–420.  https://doi.org/10.1016/j.biortech.2012.08.049 CrossRefGoogle Scholar
  32. Li P, Wang S, Peng Y, Liu Y, He J (2015) The synergistic effects of dissolved oxygen and pH on N2O production in biological domestic wastewater treatment under nitrifying conditions. Environ Technol 36:1623–1631.  https://doi.org/10.1080/09593330.2014.1002862 CrossRefGoogle Scholar
  33. Liang W, Yu C, Ren H, Geng J, Ding L, Xu K (2015) Minimization of nitrous oxide emission from CASS process treating low carbon source domestic wastewater: effect of feeding strategy and aeration rate. Bioresour Technol 198:172–180.  https://doi.org/10.1016/j.biortech.2015.08.075 CrossRefGoogle Scholar
  34. Lim JW, Lim PE, Seng CE, Adnan R (2013) Evaluation of aeration strategy in moving bed sequencing batch reactor performing simultaneous 4-chlorophenol and nitrogen removal. Appl Biochem Biotechnol 170:831–840.  https://doi.org/10.1007/s12010-013-0245-8 CrossRefGoogle Scholar
  35. Liwarska-Bizukojc E, Chojnacki J, Klink M et al (2018) Effect of the type of the external carbon source on denitrification kinetics of wastewater. Desalin Water Treat 101:143–150.  https://doi.org/10.5004/dwt.2018.21758 CrossRefGoogle Scholar
  36. Louzeiro NR, Mavinic DS, Oldham WK, Meisen A, Gardner IS (2003) Process control and design considerations for methanol-induced denitrification in a sequencing batch reactor. Environ Technol 24:161–169.  https://doi.org/10.1080/09593330309385547 CrossRefGoogle Scholar
  37. Lu H, Chandran K (2010) Factors promoting emissions of nitrous oxide and nitric oxide from denitrifying sequencing batch reactors operated with methanol and ethanol as electron donors. Biotechnol Bioeng 106:390–398.  https://doi.org/10.1002/bit.22704 Google Scholar
  38. Mannina G, Morici C, Cosenza A, di Trapani D, Ødegaard H (2016) Greenhouse gases from sequential batch membrane bioreactors: a pilot plant case study. Biochem Eng J 112:114–122.  https://doi.org/10.1016/j.bej.2016.04.010 CrossRefGoogle Scholar
  39. Massara TM, Malamis S, Guisasola A, Baeza JA, Noutsopoulos C, Katsou E (2017) A review on nitrous oxide (N2O) emissions during biological nutrient removal from municipal wastewater and sludge reject water. Sci Total Environ 596:106–123.  https://doi.org/10.1016/j.scitotenv.2017.03.191 CrossRefGoogle Scholar
  40. Ni BJ, Ye L, Law Y, Byers C, Yuan Z (2013) Mathematical modeling of nitrous oxide (N2O) emissions from full-scale wastewater treatment plants. Environ Sci Technol 47:7795–7803.  https://doi.org/10.1021/es4005398 CrossRefGoogle Scholar
  41. Ni BJ, Yuan Z (2015) Recent advances in mathematical modeling of nitrous oxides emissions from wastewater treatment processes. Water Res 87:336–346.  https://doi.org/10.1016/j.watres.2015.09.049 CrossRefGoogle Scholar
  42. Pijuan M, Tora J, Rodriguez-Caballero A et al (2014) Effect of process parameters and operational mode on nitrous oxide emissions from a nitritation reactor treating reject wastewater. Water Res 49:23–33.  https://doi.org/10.1016/j.watres.2013.11.009 CrossRefGoogle Scholar
  43. Poh LS, Jiang X, Zhang Z, Liu Y, Ng WJ, Zhou Y (2015) N2O accumulation from denitrification under different temperatures. Appl Microbiol Biotechnol 99:9215–9226.  https://doi.org/10.1007/s00253-015-6742-7 CrossRefGoogle Scholar
  44. Poughon L, Dussap CG, Gros JB (2001) Energy model and metabolic flux analysis for autotrophic nitrifiers. Biotechnol Bioeng 72:416–433.  https://doi.org/10.1002/1097-0290(20000220)72:4<416::aid-bit1004>3.3.co;2-4 CrossRefGoogle Scholar
  45. Quan X, Zhang M, Lawlor PG, Yang Z, Zhan X (2012) Nitrous oxide emission and nutrient removal in aerobic granular sludge sequencing batch reactors. Water Res 46:4981–4990.  https://doi.org/10.1016/j.watres.2012.06.031 CrossRefGoogle Scholar
  46. Ravishankara AR, Daniel JS, Portmann RW (2009) Nitrous oxide (N2O): the dominant ozone-depleting substance emitted in the 21st century. Science 326:123–125.  https://doi.org/10.1126/science.1176985 CrossRefGoogle Scholar
  47. Rodriguez-Caballero A, Aymerich I, Marques R, Poch M, Pijuan M (2015) Minimizing N2O emissions and carbon footprint on a full-scale activated sludge sequencing batch reactor. Water Res 71:1–10.  https://doi.org/10.1016/j.watres.2014.12.032 CrossRefGoogle Scholar
  48. Shiskowski DM, Mavinic DS (2006) The influence of nitrite and pH (nitrous acid) on aerobic-phase, autotrophic N2O generation in a wastewater treatment bioreactor. J Environ Eng Sci 5:273–283.  https://doi.org/10.1139/S05-034 CrossRefGoogle Scholar
  49. Tallec G, Garnier J, Billen G, Gousailles M (2006) Nitrous oxide emissions from secondary activated sludge in nitrifying conditions of urban wastewater treatment plants: effect of oxygenation level. Water Res 40:2972–2980.  https://doi.org/10.1016/j.watres.2006.05.037 CrossRefGoogle Scholar
  50. Tan TW, Ng HY (2008) Influence of mixed liquor recycle ratio and dissolved oxygen on performance of pre-denitrification submerged membrane bioreactors. Water Res 42:1122–1132.  https://doi.org/10.1016/j.watres.2007.08.028 CrossRefGoogle Scholar
  51. Thaler KM, Berger C, Leix C, Drewes J, Niessner R, Haisch C (2017) Photoacoustic spectroscopy for the quantification of N2O in the off gas of wastewater treatment plants. Anal Chem 89:3795–3801.  https://doi.org/10.1021/acs.analchem.7b00491 CrossRefGoogle Scholar
  52. Tumendelger A, Toyoda S, Yoshida N (2014) Isotopic analysis of N2O produced in a conventional wastewater treatment system operated under different aeration conditions. Rapid Commun Mass Spectrom 28:1883–1892.  https://doi.org/10.1002/rcm.6973 CrossRefGoogle Scholar
  53. Waki M, Yasuda T, Yokoyama H, Hanajima D, Ogino A, Suzuki K, Yamagishi T, Suwa Y, Tanaka Y (2009) Nitrogen removal by co-occurring methane oxidation, denitrification, aerobic ammonium oxidation, and anammox. Appl Microbiol Biotechnol 84:977–985.  https://doi.org/10.1007/s00253-009-2112-7 CrossRefGoogle Scholar
  54. Wuebbles DJ (2009) Nitrous oxide: no laughing matter. Science 326:56–57.  https://doi.org/10.1126/science.1179571 CrossRefGoogle Scholar
  55. Wunderlin P, Mohn J, Joss A, Emmenegger L, Siegrist H (2012) Mechanisms of N2O production in biological wastewater treatment under nitrifying and denitrifying conditions. Water Res 46:1027–1037.  https://doi.org/10.1016/j.watres.2011.11.080 CrossRefGoogle Scholar
  56. Wunderlin P, Lehmann MF, Siegrist H, Tuzson B, Joss A, Emmenegger L, Mohn J (2013) Isotope signatures of N2O in a mixed microbial population system: constraints on N2O producing pathways in wastewater treatment. Environ Sci Technol 47:1339–1348.  https://doi.org/10.1021/es303174x CrossRefGoogle Scholar
  57. Xiang Y, Shao Z, Kang W, Zou B, Chai H (2016) Effect of biofilm density on nitrous oxide emissions and treatment efficiency on sequencing batch biofilm reactor. Water Air Soil Pollut 227.  https://doi.org/10.1007/s11270-016-3009-6
  58. Yu R, Kampschreur MJ, van Loosdrecht MCM et al (2010) Mechanisms and specific directionality of autotrophic nitrous oxide and nitric oxide generation during transient anoxia. Environ Sci Technol 44:1313–1319.  https://doi.org/10.1021/es902794a CrossRefGoogle Scholar
  59. Zhao Y, Miao J, Ren X, Wu G (2018) Effect of organic carbon on the production of biofuel nitrous oxide during the denitrification process. Int J Environ Sci Technol 15:461–470.  https://doi.org/10.1007/s13762-017-1397-9 CrossRefGoogle Scholar
  60. Zhou Y, Pijuan M, Zeng RJ, Yuan Z (2008) Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge. Environ Sci Technol 42:8260–8265.  https://doi.org/10.1021/es800650j CrossRefGoogle Scholar
  61. Zhou Y, Lim M, Harjono S, Ng WJ (2012) Nitrous oxide emission by denitrifying phosphorus removal culture using polyhydroxyalkanoates as carbon source. J Environ Sci-China 24:1616–1623.  https://doi.org/10.1016/s1001-0742(11)60996-0 CrossRefGoogle Scholar
  62. Zhu S, Zheng M, Li C, Gui M, Chen Q, Ni J (2015) Special role of corn flour as an ideal carbon source for aerobic denitrification with minimized nitrous oxide emission. Bioresour Technol 186:44–51.  https://doi.org/10.1016/j.biortech.2015.03.046 CrossRefGoogle Scholar
  63. Zhu X, Chen Y (2011) Reduction of N2O and NO generation in anaerobic-aerobic (low dissolved oxygen) biological wastewater treatment process by using sludge alkaline fermentation liquid. Environ Sci Technol 45:2137–2143.  https://doi.org/10.1021/es102900h CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry of EducationChongqing UniversityChongqingPeople’s Republic of China
  2. 2.National Centre for International Research of Low-carbon and Green BuildingsChongqing UniversityChongqingPeople’s Republic of China
  3. 3.College of PhysicsChongqing UniversityChongqingPeople’s Republic of China

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